Detection ofpap-,sfa- andafa-specific DNA sequences inEscherichia coli strains isolated from extraintestinal material
P-fimbriae, S-fimbriae and AFA-adhesins are virulence factors responsible for adherence ofEscherichia coli strains to extraintestinal host-cell surface. Detection ofpap-,sfa- andafa-specific sequences performed by PCR revealed 74%pap+, 65%sfa+, and 8.3%afa+ strains in a group of 84 extraintestialE....
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| Veröffentlicht in: | Folia microbiologica 2002-12, Vol.47 (6), p.723-726 |
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| container_title | Folia microbiologica |
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| creator | Bogyiová, E. Kmeťová, M. Biroš, E. Siegfried, L. |
| description | P-fimbriae, S-fimbriae and AFA-adhesins are virulence factors responsible for adherence ofEscherichia coli strains to extraintestinal host-cell surface. Detection ofpap-,sfa- andafa-specific sequences performed by PCR revealed 74%pap+, 65%sfa+, and 8.3%afa+ strains in a group of 84 extraintestialE. coli isolates. Detection in a group of fecal strains showed 29%pap+, 21%sfa+ and 4%afa+ strains.pap together withsfa were found as the most frequent combination (56%) among extraintestinal isolates probably due to localization ofpap-andsfa-operons on a common pathogenicity island. The occurrence ofafa-specific sequence among 56 urine strains was 11%, although noafa+ strain was detected among 28 gynecological isolates. No strains with detected adhesin operons were found among twenty (24%) extraintestinalE. coli strains. |
| doi_str_mv | 10.1007/BF02818678 |
| format | Article |
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Detection ofpap-,sfa- andafa-specific sequences performed by PCR revealed 74%pap+, 65%sfa+, and 8.3%afa+ strains in a group of 84 extraintestialE. coli isolates. Detection in a group of fecal strains showed 29%pap+, 21%sfa+ and 4%afa+ strains.pap together withsfa were found as the most frequent combination (56%) among extraintestinal isolates probably due to localization ofpap-andsfa-operons on a common pathogenicity island. The occurrence ofafa-specific sequence among 56 urine strains was 11%, although noafa+ strain was detected among 28 gynecological isolates. 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| subjects | Adhesins Bacteria Bacteriology Cell surface Gene sequencing Localization Nucleotide sequence Operons Pathogenicity Pathogens Pili Virulence factors |
| title | Detection ofpap-,sfa- andafa-specific DNA sequences inEscherichia coli strains isolated from extraintestinal material |
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