Detection of human papillomavirus infection in tissue blocks by in situ hybridization as compared with a polymerase chain reaction procedure
Tissue blocks from 25 cases of condyloma and/or dysplasia were used for human papillomavirus typing with DNA in situ hybridization, compared with the very sensitive polymerase chain reaction. Only one of these cases was negative with both methods; a case of vaginal “koilocytosis”. Polymerase chain r...
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| Veröffentlicht in: | Human pathology 1991-06, Vol.22 (6), p.578-582 |
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| container_title | Human pathology |
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| creator | Skyldberg, Barbro Kalantari, Mina Kärkl, Marja Johansson, Bo Hagmar, Björn Walaas, Lisa |
| description | Tissue blocks from 25 cases of condyloma and/or dysplasia were used for human papillomavirus typing with DNA in situ hybridization, compared with the very sensitive polymerase chain reaction. Only one of these cases was negative with both methods; a case of vaginal “koilocytosis”. Polymerase chain reaction, as expected, was the more sensitive method, positive in 24 cases, with seven double infections. In situ hybridization was positive in 18 cases, with only two detected double infections. There was excellent agreement between the two methods in typing results. In all cases in situ hybridization showed a positive reaction in areas of koilocytosis and/or dysplasia. |
| doi_str_mv | 10.1016/0046-8177(91)90235-H |
| format | Article |
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Only one of these cases was negative with both methods; a case of vaginal “koilocytosis”. Polymerase chain reaction, as expected, was the more sensitive method, positive in 24 cases, with seven double infections. In situ hybridization was positive in 18 cases, with only two detected double infections. There was excellent agreement between the two methods in typing results. 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Only one of these cases was negative with both methods; a case of vaginal “koilocytosis”. Polymerase chain reaction, as expected, was the more sensitive method, positive in 24 cases, with seven double infections. In situ hybridization was positive in 18 cases, with only two detected double infections. There was excellent agreement between the two methods in typing results. In all cases in situ hybridization showed a positive reaction in areas of koilocytosis and/or dysplasia.</description><subject>Biological and medical sciences</subject><subject>Biopsy</subject><subject>Condylomata Acuminata - microbiology</subject><subject>Condylomata Acuminata - pathology</subject><subject>DNA, Viral - analysis</subject><subject>DNA, Viral - genetics</subject><subject>Female</subject><subject>Genital Diseases, Female - microbiology</subject><subject>Genital Diseases, Female - pathology</subject><subject>Genital Neoplasms, Female - microbiology</subject><subject>Genital Neoplasms, Female - pathology</subject><subject>human papillomavirus</subject><subject>Humans</subject><subject>in situ hybridization</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Medical sciences</subject><subject>Nucleic Acid Hybridization</subject><subject>Papillomaviridae - classification</subject><subject>Papillomaviridae - isolation & purification</subject><subject>paraffin sections</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Polymerase Chain Reaction</subject><subject>Tumor Virus Infections - diagnosis</subject><issn>0046-8177</issn><issn>1532-8392</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU-L1TAUxYMo43P0GyhkIaKLatI0fclGkPHPEwbc6DrcJDe8aNvUpB15fgY_tO30Me5cBXJ_59zLOYQ85ew1Z7x9w1jTVorv9y81f6VZLWR1uEd2XIq6UkLX98nuDnlIHpXynTHOZSMvyAVvJdtLuSN_3uOEboppoCnQ49zDQEcYY9elHm5inguNQzgTcaBTLGVGarvkfhRqT-tfidNMjyebo4-_4ZaEQl3qR8jo6a84HSnQMXWnHjMUpO4IiywjbLZjTg79nPExeRCgK_jk_F6Sbx8_fL06VNdfPn2-enddOcH4VHmAFmpWI3KunBIOteXWKRVE0NzagEG3XjMvWS0VclW32kvRQMMtKCHFJXmx-S6bf85YJtPH4rDrYMA0F6PYvm6VUgvYbKDLqZSMwYw59pBPhjOzlmDWhM2asNHc3JZgDovs2dl_tj36f6It9WX-_DyH4qALGQYXyx0mhZYNW7e_3TBcsriJmE1xEYclq5iXRoxP8f93_AXMGaam</recordid><startdate>199106</startdate><enddate>199106</enddate><creator>Skyldberg, Barbro</creator><creator>Kalantari, Mina</creator><creator>Kärkl, Marja</creator><creator>Johansson, Bo</creator><creator>Hagmar, Björn</creator><creator>Walaas, Lisa</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199106</creationdate><title>Detection of human papillomavirus infection in tissue blocks by in situ hybridization as compared with a polymerase chain reaction procedure</title><author>Skyldberg, Barbro ; Kalantari, Mina ; Kärkl, Marja ; Johansson, Bo ; Hagmar, Björn ; Walaas, Lisa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c301t-daa6a202ee118c83ce9b1bc88f3f91bbfef96d90d50258e18269d534a41ba8353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Biological and medical sciences</topic><topic>Biopsy</topic><topic>Condylomata Acuminata - microbiology</topic><topic>Condylomata Acuminata - pathology</topic><topic>DNA, Viral - analysis</topic><topic>DNA, Viral - genetics</topic><topic>Female</topic><topic>Genital Diseases, Female - microbiology</topic><topic>Genital Diseases, Female - pathology</topic><topic>Genital Neoplasms, Female - microbiology</topic><topic>Genital Neoplasms, Female - pathology</topic><topic>human papillomavirus</topic><topic>Humans</topic><topic>in situ hybridization</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Medical sciences</topic><topic>Nucleic Acid Hybridization</topic><topic>Papillomaviridae - classification</topic><topic>Papillomaviridae - isolation & purification</topic><topic>paraffin sections</topic><topic>Pathology. 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Miscellaneous investigative techniques</topic><topic>Polymerase Chain Reaction</topic><topic>Tumor Virus Infections - diagnosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Skyldberg, Barbro</creatorcontrib><creatorcontrib>Kalantari, Mina</creatorcontrib><creatorcontrib>Kärkl, Marja</creatorcontrib><creatorcontrib>Johansson, Bo</creatorcontrib><creatorcontrib>Hagmar, Björn</creatorcontrib><creatorcontrib>Walaas, Lisa</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Human pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Skyldberg, Barbro</au><au>Kalantari, Mina</au><au>Kärkl, Marja</au><au>Johansson, Bo</au><au>Hagmar, Björn</au><au>Walaas, Lisa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of human papillomavirus infection in tissue blocks by in situ hybridization as compared with a polymerase chain reaction procedure</atitle><jtitle>Human pathology</jtitle><addtitle>Hum Pathol</addtitle><date>1991-06</date><risdate>1991</risdate><volume>22</volume><issue>6</issue><spage>578</spage><epage>582</epage><pages>578-582</pages><issn>0046-8177</issn><eissn>1532-8392</eissn><coden>HPCQA4</coden><abstract>Tissue blocks from 25 cases of condyloma and/or dysplasia were used for human papillomavirus typing with DNA in situ hybridization, compared with the very sensitive polymerase chain reaction. Only one of these cases was negative with both methods; a case of vaginal “koilocytosis”. Polymerase chain reaction, as expected, was the more sensitive method, positive in 24 cases, with seven double infections. In situ hybridization was positive in 18 cases, with only two detected double infections. There was excellent agreement between the two methods in typing results. In all cases in situ hybridization showed a positive reaction in areas of koilocytosis and/or dysplasia.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>1650755</pmid><doi>10.1016/0046-8177(91)90235-H</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biological and medical sciences Biopsy Condylomata Acuminata - microbiology Condylomata Acuminata - pathology DNA, Viral - analysis DNA, Viral - genetics Female Genital Diseases, Female - microbiology Genital Diseases, Female - pathology Genital Neoplasms, Female - microbiology Genital Neoplasms, Female - pathology human papillomavirus Humans in situ hybridization Investigative techniques, diagnostic techniques (general aspects) Medical sciences Nucleic Acid Hybridization Papillomaviridae - classification Papillomaviridae - isolation & purification paraffin sections Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Polymerase Chain Reaction Tumor Virus Infections - diagnosis |
| title | Detection of human papillomavirus infection in tissue blocks by in situ hybridization as compared with a polymerase chain reaction procedure |
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