Genome-wide functional analysis of pathogenicity genes in the rice blast fungus
Rapid translation of genome sequences into meaningful biological information hinges on the integration of multiple experimental and informatics methods into a cohesive platform. Despite the explosion in the number of genome sequences available 1 , such a platform does not exist for filamentous fungi...
Gespeichert in:
| Veröffentlicht in: | Nature genetics 2007-04, Vol.39 (4), p.561-565 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 565 |
|---|---|
| container_issue | 4 |
| container_start_page | 561 |
| container_title | Nature genetics |
| container_volume | 39 |
| creator | Jeon, Junhyun Park, Sook-Young Chi, Myoung-Hwan Choi, Jaehyuk Park, Jongsun Rho, Hee-Sool Kim, Soonok Goh, Jaeduk Yoo, Sungyong Choi, Jinhee Park, Ju-Young Yi, Mihwa Yang, Seonyoung Kwon, Min-Jung Han, Seong-Sook Kim, Byeong Ryun Khang, Chang Hyun Park, Bongsoo Lim, Se-Eun Jung, Kyongyong Kong, Sunghyung Karunakaran, Maruthachalam Oh, Hong-Sik Kim, Hyojeong Kim, Seryun Park, Jaejin Kang, Soyoung Choi, Woo-Bong Kang, Seogchan Lee, Yong-Hwan |
| description | Rapid translation of genome sequences into meaningful biological information hinges on the integration of multiple experimental and informatics methods into a cohesive platform. Despite the explosion in the number of genome sequences available
1
, such a platform does not exist for filamentous fungi. Here we present the development and application of a functional genomics and informatics platform for a model plant pathogenic fungus,
Magnaporthe oryzae
2
. In total, we produced 21,070 mutants through large-scale insertional mutagenesis using
Agrobacterium tumefaciens
–mediated transformation
3
. We used a high-throughput phenotype screening pipeline to detect disruption of seven phenotypes encompassing the fungal life cycle and identified the mutated gene and the nature of mutation for each mutant. Comparative analysis of phenotypes and genotypes of the mutants uncovered 202 new pathogenicity loci. Our findings demonstrate the effectiveness of our platform and provide new insights on the molecular basis of fungal pathogenesis. Our approach promises comprehensive functional genomics in filamentous fungi and beyond. |
| doi_str_mv | 10.1038/ng2002 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_807266516</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A183393569</galeid><sourcerecordid>A183393569</sourcerecordid><originalsourceid>FETCH-LOGICAL-c630t-9ad35cc564cc90b73761e448b53484b5d62299afdb6a1e0fef2388660c16ca73</originalsourceid><addsrcrecordid>eNqN0v9r1DAUAPAiiptT_wQpDhV_6My3vqY_jqFzMDjQ4a8lTV-6jF5yJil6_705rnDcEJFAEpLPS8jLK4rXlFxQwuUnNzJC2JPilNYCKtpQ-TTPCdBKEA4nxYsYHwihQhD5vDihDa-5bMVpsbpG59dY_bIDlmZ2Olnv1FSq3G2jjaU35Ualez-is9qmbZknGEvrynSPZbAay35SMe2Cxzm-LJ4ZNUV8tYxnxd2Xz3dXX6vb1fXN1eVtpYGTVLVq4LXWNQitW9I3vAGKQsi-5kKKvh6AsbZVZuhBUSQGDeNSAhBNQauGnxUf9sdugv85Y0zd2kaN06Qc-jl2kjQMoKaQ5ft_SkbkLi8kw7eP4IOfQ05DNoyBAAkyo_M9GtWEnXXGp6D07sTukkrOW15Dm9XFX1RuA66t9g6NzetHAR-PArJJ-DuNao6xu_n-7f_t6sexXR6vg48xoOk2wa5V2HaUdLu66fZ1k-Gb5fFzv8bhwJZCyeDdAlTUajJBOW3jwUloOKPt4WNi3nIjhkMWH135B8rq0jg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>222646868</pqid></control><display><type>article</type><title>Genome-wide functional analysis of pathogenicity genes in the rice blast fungus</title><source>MEDLINE</source><source>SpringerLink Journals</source><source>Nature Journals Online</source><creator>Jeon, Junhyun ; Park, Sook-Young ; Chi, Myoung-Hwan ; Choi, Jaehyuk ; Park, Jongsun ; Rho, Hee-Sool ; Kim, Soonok ; Goh, Jaeduk ; Yoo, Sungyong ; Choi, Jinhee ; Park, Ju-Young ; Yi, Mihwa ; Yang, Seonyoung ; Kwon, Min-Jung ; Han, Seong-Sook ; Kim, Byeong Ryun ; Khang, Chang Hyun ; Park, Bongsoo ; Lim, Se-Eun ; Jung, Kyongyong ; Kong, Sunghyung ; Karunakaran, Maruthachalam ; Oh, Hong-Sik ; Kim, Hyojeong ; Kim, Seryun ; Park, Jaejin ; Kang, Soyoung ; Choi, Woo-Bong ; Kang, Seogchan ; Lee, Yong-Hwan</creator><creatorcontrib>Jeon, Junhyun ; Park, Sook-Young ; Chi, Myoung-Hwan ; Choi, Jaehyuk ; Park, Jongsun ; Rho, Hee-Sool ; Kim, Soonok ; Goh, Jaeduk ; Yoo, Sungyong ; Choi, Jinhee ; Park, Ju-Young ; Yi, Mihwa ; Yang, Seonyoung ; Kwon, Min-Jung ; Han, Seong-Sook ; Kim, Byeong Ryun ; Khang, Chang Hyun ; Park, Bongsoo ; Lim, Se-Eun ; Jung, Kyongyong ; Kong, Sunghyung ; Karunakaran, Maruthachalam ; Oh, Hong-Sik ; Kim, Hyojeong ; Kim, Seryun ; Park, Jaejin ; Kang, Soyoung ; Choi, Woo-Bong ; Kang, Seogchan ; Lee, Yong-Hwan</creatorcontrib><description>Rapid translation of genome sequences into meaningful biological information hinges on the integration of multiple experimental and informatics methods into a cohesive platform. Despite the explosion in the number of genome sequences available
1
, such a platform does not exist for filamentous fungi. Here we present the development and application of a functional genomics and informatics platform for a model plant pathogenic fungus,
Magnaporthe oryzae
2
. In total, we produced 21,070 mutants through large-scale insertional mutagenesis using
Agrobacterium tumefaciens
–mediated transformation
3
. We used a high-throughput phenotype screening pipeline to detect disruption of seven phenotypes encompassing the fungal life cycle and identified the mutated gene and the nature of mutation for each mutant. Comparative analysis of phenotypes and genotypes of the mutants uncovered 202 new pathogenicity loci. Our findings demonstrate the effectiveness of our platform and provide new insights on the molecular basis of fungal pathogenesis. Our approach promises comprehensive functional genomics in filamentous fungi and beyond.</description><identifier>ISSN: 1061-4036</identifier><identifier>EISSN: 1546-1718</identifier><identifier>DOI: 10.1038/ng2002</identifier><identifier>PMID: 17353894</identifier><identifier>CODEN: NGENEC</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>Agriculture ; Agrobacterium ; Agrobacterium tumefaciens - genetics ; Animal Genetics and Genomics ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Biotechnology ; Cancer Research ; Causes of ; Chromosome Mapping ; Chromosomes, Fungal ; Crop science ; DNA sequencing ; Fundamental and applied biological sciences. Psychology ; Fungi ; Fungi, Phytopathogenic ; Gene Function ; Genes, Fungal - physiology ; Genetic aspects ; Genetics of eukaryotes. Biological and molecular evolution ; Genome, Fungal ; Genomics ; Genotype ; Genotype & phenotype ; Genotypes ; Human Genetics ; letter ; Life cycles ; Magnaporthe - genetics ; Magnaporthe oryzae ; Methods ; Models, Biological ; Mutants ; Mutation ; Nucleotide sequencing ; Organisms, Genetically Modified ; Oryza sativa ; Pathogens ; Phenotype ; Physiological aspects ; Rice blast disease ; Virulence Factors - genetics ; Virulence Factors - isolation & purification ; Virulence Factors - physiology</subject><ispartof>Nature genetics, 2007-04, Vol.39 (4), p.561-565</ispartof><rights>Springer Nature America, Inc. 2007</rights><rights>2007 INIST-CNRS</rights><rights>COPYRIGHT 2007 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Apr 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c630t-9ad35cc564cc90b73761e448b53484b5d62299afdb6a1e0fef2388660c16ca73</citedby><cites>FETCH-LOGICAL-c630t-9ad35cc564cc90b73761e448b53484b5d62299afdb6a1e0fef2388660c16ca73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/ng2002$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/ng2002$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18673219$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17353894$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jeon, Junhyun</creatorcontrib><creatorcontrib>Park, Sook-Young</creatorcontrib><creatorcontrib>Chi, Myoung-Hwan</creatorcontrib><creatorcontrib>Choi, Jaehyuk</creatorcontrib><creatorcontrib>Park, Jongsun</creatorcontrib><creatorcontrib>Rho, Hee-Sool</creatorcontrib><creatorcontrib>Kim, Soonok</creatorcontrib><creatorcontrib>Goh, Jaeduk</creatorcontrib><creatorcontrib>Yoo, Sungyong</creatorcontrib><creatorcontrib>Choi, Jinhee</creatorcontrib><creatorcontrib>Park, Ju-Young</creatorcontrib><creatorcontrib>Yi, Mihwa</creatorcontrib><creatorcontrib>Yang, Seonyoung</creatorcontrib><creatorcontrib>Kwon, Min-Jung</creatorcontrib><creatorcontrib>Han, Seong-Sook</creatorcontrib><creatorcontrib>Kim, Byeong Ryun</creatorcontrib><creatorcontrib>Khang, Chang Hyun</creatorcontrib><creatorcontrib>Park, Bongsoo</creatorcontrib><creatorcontrib>Lim, Se-Eun</creatorcontrib><creatorcontrib>Jung, Kyongyong</creatorcontrib><creatorcontrib>Kong, Sunghyung</creatorcontrib><creatorcontrib>Karunakaran, Maruthachalam</creatorcontrib><creatorcontrib>Oh, Hong-Sik</creatorcontrib><creatorcontrib>Kim, Hyojeong</creatorcontrib><creatorcontrib>Kim, Seryun</creatorcontrib><creatorcontrib>Park, Jaejin</creatorcontrib><creatorcontrib>Kang, Soyoung</creatorcontrib><creatorcontrib>Choi, Woo-Bong</creatorcontrib><creatorcontrib>Kang, Seogchan</creatorcontrib><creatorcontrib>Lee, Yong-Hwan</creatorcontrib><title>Genome-wide functional analysis of pathogenicity genes in the rice blast fungus</title><title>Nature genetics</title><addtitle>Nat Genet</addtitle><addtitle>Nat Genet</addtitle><description>Rapid translation of genome sequences into meaningful biological information hinges on the integration of multiple experimental and informatics methods into a cohesive platform. Despite the explosion in the number of genome sequences available
1
, such a platform does not exist for filamentous fungi. Here we present the development and application of a functional genomics and informatics platform for a model plant pathogenic fungus,
Magnaporthe oryzae
2
. In total, we produced 21,070 mutants through large-scale insertional mutagenesis using
Agrobacterium tumefaciens
–mediated transformation
3
. We used a high-throughput phenotype screening pipeline to detect disruption of seven phenotypes encompassing the fungal life cycle and identified the mutated gene and the nature of mutation for each mutant. Comparative analysis of phenotypes and genotypes of the mutants uncovered 202 new pathogenicity loci. Our findings demonstrate the effectiveness of our platform and provide new insights on the molecular basis of fungal pathogenesis. Our approach promises comprehensive functional genomics in filamentous fungi and beyond.</description><subject>Agriculture</subject><subject>Agrobacterium</subject><subject>Agrobacterium tumefaciens - genetics</subject><subject>Animal Genetics and Genomics</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Cancer Research</subject><subject>Causes of</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Fungal</subject><subject>Crop science</subject><subject>DNA sequencing</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungi</subject><subject>Fungi, Phytopathogenic</subject><subject>Gene Function</subject><subject>Genes, Fungal - physiology</subject><subject>Genetic aspects</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Genome, Fungal</subject><subject>Genomics</subject><subject>Genotype</subject><subject>Genotype & phenotype</subject><subject>Genotypes</subject><subject>Human Genetics</subject><subject>letter</subject><subject>Life cycles</subject><subject>Magnaporthe - genetics</subject><subject>Magnaporthe oryzae</subject><subject>Methods</subject><subject>Models, Biological</subject><subject>Mutants</subject><subject>Mutation</subject><subject>Nucleotide sequencing</subject><subject>Organisms, Genetically Modified</subject><subject>Oryza sativa</subject><subject>Pathogens</subject><subject>Phenotype</subject><subject>Physiological aspects</subject><subject>Rice blast disease</subject><subject>Virulence Factors - genetics</subject><subject>Virulence Factors - isolation & purification</subject><subject>Virulence Factors - physiology</subject><issn>1061-4036</issn><issn>1546-1718</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqN0v9r1DAUAPAiiptT_wQpDhV_6My3vqY_jqFzMDjQ4a8lTV-6jF5yJil6_705rnDcEJFAEpLPS8jLK4rXlFxQwuUnNzJC2JPilNYCKtpQ-TTPCdBKEA4nxYsYHwihQhD5vDihDa-5bMVpsbpG59dY_bIDlmZ2Olnv1FSq3G2jjaU35Ualez-is9qmbZknGEvrynSPZbAay35SMe2Cxzm-LJ4ZNUV8tYxnxd2Xz3dXX6vb1fXN1eVtpYGTVLVq4LXWNQitW9I3vAGKQsi-5kKKvh6AsbZVZuhBUSQGDeNSAhBNQauGnxUf9sdugv85Y0zd2kaN06Qc-jl2kjQMoKaQ5ft_SkbkLi8kw7eP4IOfQ05DNoyBAAkyo_M9GtWEnXXGp6D07sTukkrOW15Dm9XFX1RuA66t9g6NzetHAR-PArJJ-DuNao6xu_n-7f_t6sexXR6vg48xoOk2wa5V2HaUdLu66fZ1k-Gb5fFzv8bhwJZCyeDdAlTUajJBOW3jwUloOKPt4WNi3nIjhkMWH135B8rq0jg</recordid><startdate>20070401</startdate><enddate>20070401</enddate><creator>Jeon, Junhyun</creator><creator>Park, Sook-Young</creator><creator>Chi, Myoung-Hwan</creator><creator>Choi, Jaehyuk</creator><creator>Park, Jongsun</creator><creator>Rho, Hee-Sool</creator><creator>Kim, Soonok</creator><creator>Goh, Jaeduk</creator><creator>Yoo, Sungyong</creator><creator>Choi, Jinhee</creator><creator>Park, Ju-Young</creator><creator>Yi, Mihwa</creator><creator>Yang, Seonyoung</creator><creator>Kwon, Min-Jung</creator><creator>Han, Seong-Sook</creator><creator>Kim, Byeong Ryun</creator><creator>Khang, Chang Hyun</creator><creator>Park, Bongsoo</creator><creator>Lim, Se-Eun</creator><creator>Jung, Kyongyong</creator><creator>Kong, Sunghyung</creator><creator>Karunakaran, Maruthachalam</creator><creator>Oh, Hong-Sik</creator><creator>Kim, Hyojeong</creator><creator>Kim, Seryun</creator><creator>Park, Jaejin</creator><creator>Kang, Soyoung</creator><creator>Choi, Woo-Bong</creator><creator>Kang, Seogchan</creator><creator>Lee, Yong-Hwan</creator><general>Nature Publishing Group US</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SS</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope></search><sort><creationdate>20070401</creationdate><title>Genome-wide functional analysis of pathogenicity genes in the rice blast fungus</title><author>Jeon, Junhyun ; Park, Sook-Young ; Chi, Myoung-Hwan ; Choi, Jaehyuk ; Park, Jongsun ; Rho, Hee-Sool ; Kim, Soonok ; Goh, Jaeduk ; Yoo, Sungyong ; Choi, Jinhee ; Park, Ju-Young ; Yi, Mihwa ; Yang, Seonyoung ; Kwon, Min-Jung ; Han, Seong-Sook ; Kim, Byeong Ryun ; Khang, Chang Hyun ; Park, Bongsoo ; Lim, Se-Eun ; Jung, Kyongyong ; Kong, Sunghyung ; Karunakaran, Maruthachalam ; Oh, Hong-Sik ; Kim, Hyojeong ; Kim, Seryun ; Park, Jaejin ; Kang, Soyoung ; Choi, Woo-Bong ; Kang, Seogchan ; Lee, Yong-Hwan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c630t-9ad35cc564cc90b73761e448b53484b5d62299afdb6a1e0fef2388660c16ca73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Agriculture</topic><topic>Agrobacterium</topic><topic>Agrobacterium tumefaciens - genetics</topic><topic>Animal Genetics and Genomics</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Cancer Research</topic><topic>Causes of</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Fungal</topic><topic>Crop science</topic><topic>DNA sequencing</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungi</topic><topic>Fungi, Phytopathogenic</topic><topic>Gene Function</topic><topic>Genes, Fungal - physiology</topic><topic>Genetic aspects</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Genome, Fungal</topic><topic>Genomics</topic><topic>Genotype</topic><topic>Genotype & phenotype</topic><topic>Genotypes</topic><topic>Human Genetics</topic><topic>letter</topic><topic>Life cycles</topic><topic>Magnaporthe - genetics</topic><topic>Magnaporthe oryzae</topic><topic>Methods</topic><topic>Models, Biological</topic><topic>Mutants</topic><topic>Mutation</topic><topic>Nucleotide sequencing</topic><topic>Organisms, Genetically Modified</topic><topic>Oryza sativa</topic><topic>Pathogens</topic><topic>Phenotype</topic><topic>Physiological aspects</topic><topic>Rice blast disease</topic><topic>Virulence Factors - genetics</topic><topic>Virulence Factors - isolation & purification</topic><topic>Virulence Factors - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jeon, Junhyun</creatorcontrib><creatorcontrib>Park, Sook-Young</creatorcontrib><creatorcontrib>Chi, Myoung-Hwan</creatorcontrib><creatorcontrib>Choi, Jaehyuk</creatorcontrib><creatorcontrib>Park, Jongsun</creatorcontrib><creatorcontrib>Rho, Hee-Sool</creatorcontrib><creatorcontrib>Kim, Soonok</creatorcontrib><creatorcontrib>Goh, Jaeduk</creatorcontrib><creatorcontrib>Yoo, Sungyong</creatorcontrib><creatorcontrib>Choi, Jinhee</creatorcontrib><creatorcontrib>Park, Ju-Young</creatorcontrib><creatorcontrib>Yi, Mihwa</creatorcontrib><creatorcontrib>Yang, Seonyoung</creatorcontrib><creatorcontrib>Kwon, Min-Jung</creatorcontrib><creatorcontrib>Han, Seong-Sook</creatorcontrib><creatorcontrib>Kim, Byeong Ryun</creatorcontrib><creatorcontrib>Khang, Chang Hyun</creatorcontrib><creatorcontrib>Park, Bongsoo</creatorcontrib><creatorcontrib>Lim, Se-Eun</creatorcontrib><creatorcontrib>Jung, Kyongyong</creatorcontrib><creatorcontrib>Kong, Sunghyung</creatorcontrib><creatorcontrib>Karunakaran, Maruthachalam</creatorcontrib><creatorcontrib>Oh, Hong-Sik</creatorcontrib><creatorcontrib>Kim, Hyojeong</creatorcontrib><creatorcontrib>Kim, Seryun</creatorcontrib><creatorcontrib>Park, Jaejin</creatorcontrib><creatorcontrib>Kang, Soyoung</creatorcontrib><creatorcontrib>Choi, Woo-Bong</creatorcontrib><creatorcontrib>Kang, Seogchan</creatorcontrib><creatorcontrib>Lee, Yong-Hwan</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><jtitle>Nature genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jeon, Junhyun</au><au>Park, Sook-Young</au><au>Chi, Myoung-Hwan</au><au>Choi, Jaehyuk</au><au>Park, Jongsun</au><au>Rho, Hee-Sool</au><au>Kim, Soonok</au><au>Goh, Jaeduk</au><au>Yoo, Sungyong</au><au>Choi, Jinhee</au><au>Park, Ju-Young</au><au>Yi, Mihwa</au><au>Yang, Seonyoung</au><au>Kwon, Min-Jung</au><au>Han, Seong-Sook</au><au>Kim, Byeong Ryun</au><au>Khang, Chang Hyun</au><au>Park, Bongsoo</au><au>Lim, Se-Eun</au><au>Jung, Kyongyong</au><au>Kong, Sunghyung</au><au>Karunakaran, Maruthachalam</au><au>Oh, Hong-Sik</au><au>Kim, Hyojeong</au><au>Kim, Seryun</au><au>Park, Jaejin</au><au>Kang, Soyoung</au><au>Choi, Woo-Bong</au><au>Kang, Seogchan</au><au>Lee, Yong-Hwan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome-wide functional analysis of pathogenicity genes in the rice blast fungus</atitle><jtitle>Nature genetics</jtitle><stitle>Nat Genet</stitle><addtitle>Nat Genet</addtitle><date>2007-04-01</date><risdate>2007</risdate><volume>39</volume><issue>4</issue><spage>561</spage><epage>565</epage><pages>561-565</pages><issn>1061-4036</issn><eissn>1546-1718</eissn><coden>NGENEC</coden><abstract>Rapid translation of genome sequences into meaningful biological information hinges on the integration of multiple experimental and informatics methods into a cohesive platform. Despite the explosion in the number of genome sequences available
1
, such a platform does not exist for filamentous fungi. Here we present the development and application of a functional genomics and informatics platform for a model plant pathogenic fungus,
Magnaporthe oryzae
2
. In total, we produced 21,070 mutants through large-scale insertional mutagenesis using
Agrobacterium tumefaciens
–mediated transformation
3
. We used a high-throughput phenotype screening pipeline to detect disruption of seven phenotypes encompassing the fungal life cycle and identified the mutated gene and the nature of mutation for each mutant. Comparative analysis of phenotypes and genotypes of the mutants uncovered 202 new pathogenicity loci. Our findings demonstrate the effectiveness of our platform and provide new insights on the molecular basis of fungal pathogenesis. Our approach promises comprehensive functional genomics in filamentous fungi and beyond.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>17353894</pmid><doi>10.1038/ng2002</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1061-4036 |
| ispartof | Nature genetics, 2007-04, Vol.39 (4), p.561-565 |
| issn | 1061-4036 1546-1718 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_807266516 |
| source | MEDLINE; SpringerLink Journals; Nature Journals Online |
| subjects | Agriculture Agrobacterium Agrobacterium tumefaciens - genetics Animal Genetics and Genomics Biological and medical sciences Biomedical and Life Sciences Biomedicine Biotechnology Cancer Research Causes of Chromosome Mapping Chromosomes, Fungal Crop science DNA sequencing Fundamental and applied biological sciences. Psychology Fungi Fungi, Phytopathogenic Gene Function Genes, Fungal - physiology Genetic aspects Genetics of eukaryotes. Biological and molecular evolution Genome, Fungal Genomics Genotype Genotype & phenotype Genotypes Human Genetics letter Life cycles Magnaporthe - genetics Magnaporthe oryzae Methods Models, Biological Mutants Mutation Nucleotide sequencing Organisms, Genetically Modified Oryza sativa Pathogens Phenotype Physiological aspects Rice blast disease Virulence Factors - genetics Virulence Factors - isolation & purification Virulence Factors - physiology |
| title | Genome-wide functional analysis of pathogenicity genes in the rice blast fungus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T14%3A40%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genome-wide%20functional%20analysis%20of%20pathogenicity%20genes%20in%20the%20rice%20blast%20fungus&rft.jtitle=Nature%20genetics&rft.au=Jeon,%20Junhyun&rft.date=2007-04-01&rft.volume=39&rft.issue=4&rft.spage=561&rft.epage=565&rft.pages=561-565&rft.issn=1061-4036&rft.eissn=1546-1718&rft.coden=NGENEC&rft_id=info:doi/10.1038/ng2002&rft_dat=%3Cgale_proqu%3EA183393569%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=222646868&rft_id=info:pmid/17353894&rft_galeid=A183393569&rfr_iscdi=true |