A multiplex PCR procedure for the detection of six major virulence genes in Escherichia coli O157:H7

A multiplex PCR procedure that detects six major virulence genes, fliC, stx1, stx2, eae, rfbE, and hlyA, in Escherichia coli O157:H7 was developed. Analyses of the available sequences of the six major virulence genes and the published primers allowed us to develop the six-gene, multiplex PCR protoco...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of microbiological methods 2010-07, Vol.82 (1), p.85-89
Hauptverfasser:	Bai, Jianfa, Shi, Xiaorong, Nagaraja, T.G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cattle Cattle Diseases - microbiology Colony-forming cells E. coli O157:H7 Escherichia coli Escherichia coli Infections - microbiology Escherichia coli Infections - veterinary Escherichia coli O157 - classification Escherichia coli O157 - genetics Escherichia coli O157 - isolation & purification Escherichia coli O157 - pathogenicity Escherichia coli Proteins - genetics Humans Multiplex PCR Polymerase Chain Reaction - methods Shiga toxigenic E. coli Virulence Factors - genetics Virulence genes
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!