Evaluation of some Staphylococcus aureus iron-regulated proteins as vaccine targets
Staphylococcus aureus is an important pathogen that is responsible for a wide range of infections, including bovine mastitis. Previously, 54 genes from S. aureus that were up-regulated in an iron-restricted medium and in mice were identified. Seven of those genes were selected from five iron-acquisi...
Gespeichert in:
| Veröffentlicht in: | Veterinary immunology and immunopathology 2010-08, Vol.136 (3), p.311-318 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 318 |
|---|---|
| container_issue | 3 |
| container_start_page | 311 |
| container_title | Veterinary immunology and immunopathology |
| container_volume | 136 |
| creator | Ster, Céline Beaudoin, Frédéric Diarra, Moussa S. Jacques, Mario Malouin, François Lacasse, Pierre |
| description | Staphylococcus aureus is an important pathogen that is responsible for a wide range of infections, including bovine mastitis. Previously, 54 genes from
S. aureus that were up-regulated in an iron-restricted medium and in mice were identified. Seven of those genes were selected from five iron-acquisition systems (isd, feo, sir, sst, and fhu), and the proteins were evaluated as potential vaccine targets to prevent bovine mastitis. The antigenicity of the recombinant proteins obtained with each studied gene was evaluated in rabbits and/or cattle. Immune sera were used to test the bacterial accessibility of the native proteins. All the proteins were immunogenic in rabbits or cattle. IsdH, IsdB, FeoB and SstD were expressed on the bacterial surface, with IsdB and IsdH more expressed in an iron-restricted environment. The capacity of antibodies to prevent infection was measured in a mouse mastitis model. Preincubation of
S. aureus with serum against IsdH or with the pool of sera against IsdB, SstD and FeoB led to decreased colonization of the mouse mammary glands. Lastly, cattle immunization with IsdH induced a strong and long-lasting immune response with IgG2 production. The protein IsdH appears to be a good vaccine candidate to prevent
S. aureus bovine mastitis. |
| doi_str_mv | 10.1016/j.vetimm.2010.03.010 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_807253075</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0165242710000826</els_id><sourcerecordid>807253075</sourcerecordid><originalsourceid>FETCH-LOGICAL-c483t-7e17ef09a0776aecb7dd17be6689084b77ad7aebdb07fb83588ed2655ab95ce03</originalsourceid><addsrcrecordid>eNqFkM1O3DAURq2qqAy0b1BBdl1luI7j2NlUqhAFJCQWA2vLsW-mHiXxYDsj8fYYBViW1Sf5nvvjQ8hPCmsKtLnYrQ-Y3DiuK8hPwNY5vpAVlYKVFWf1V7LKGC-ruhLH5CTGHQDwVspv5LiCmjYtZyuyuTroYdbJ-anwfRH9iMUm6f2_58Ebb8wcCz0HzOGCn8qA23nQCW2xDz6hm3I5FgdtjJuwSDpsMcXv5KjXQ8Qfb3lKHv9ePVzelHf317eXf-5KU0uWSoFUYA-tBiEajaYT1lLRYdPIFmTdCaGt0NjZDkTfScalRFs1nOuu5QaBnZJfy9x8y9OMManRRYPDoCf0c1QSRBYBgn9KCsZaSTkXmawX0gQfY8Be7YMbdXhWFNSrd7VTi3f16l0BUzly29nbgrkb0X40vYvOwPkC9NorvQ0uqsdNnsCAyvxhqDLxeyEwKzs4DCoah5NB6wKapKx3_7_hBbacoHY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>733981557</pqid></control><display><type>article</type><title>Evaluation of some Staphylococcus aureus iron-regulated proteins as vaccine targets</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Ster, Céline ; Beaudoin, Frédéric ; Diarra, Moussa S. ; Jacques, Mario ; Malouin, François ; Lacasse, Pierre</creator><creatorcontrib>Ster, Céline ; Beaudoin, Frédéric ; Diarra, Moussa S. ; Jacques, Mario ; Malouin, François ; Lacasse, Pierre</creatorcontrib><description>Staphylococcus aureus is an important pathogen that is responsible for a wide range of infections, including bovine mastitis. Previously, 54 genes from
S. aureus that were up-regulated in an iron-restricted medium and in mice were identified. Seven of those genes were selected from five iron-acquisition systems (isd, feo, sir, sst, and fhu), and the proteins were evaluated as potential vaccine targets to prevent bovine mastitis. The antigenicity of the recombinant proteins obtained with each studied gene was evaluated in rabbits and/or cattle. Immune sera were used to test the bacterial accessibility of the native proteins. All the proteins were immunogenic in rabbits or cattle. IsdH, IsdB, FeoB and SstD were expressed on the bacterial surface, with IsdB and IsdH more expressed in an iron-restricted environment. The capacity of antibodies to prevent infection was measured in a mouse mastitis model. Preincubation of
S. aureus with serum against IsdH or with the pool of sera against IsdB, SstD and FeoB led to decreased colonization of the mouse mammary glands. Lastly, cattle immunization with IsdH induced a strong and long-lasting immune response with IgG2 production. The protein IsdH appears to be a good vaccine candidate to prevent
S. aureus bovine mastitis.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>DOI: 10.1016/j.vetimm.2010.03.010</identifier><identifier>PMID: 20416953</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; antibody formation ; antigen-antibody reactions ; antigenic variation ; bacterial colonization ; bacterial infections ; bovine mastitis ; Cattle ; culture media ; dairy cows ; Disease Models, Animal ; disease prevention ; Female ; Flow Cytometry ; gene expression ; Immune response ; immunization ; Immunization - methods ; Immunization - veterinary ; Immunoglobulin G - immunology ; Immunoglobulin Isotypes - immunology ; in vitro studies ; iron ; Iron-Regulatory Proteins - immunology ; IsdH ; Male ; mammary glands ; Mastitis ; Mastitis, Bovine - immunology ; Mastitis, Bovine - microbiology ; Mastitis, Bovine - prevention & control ; Rabbits ; recombinant proteins ; Recombinant Proteins - immunology ; Staphylococcal Infections - immunology ; Staphylococcal Infections - microbiology ; Staphylococcal Infections - veterinary ; Staphylococcus aureus ; Staphylococcus aureus - immunology ; Vaccine ; vaccine development ; vaccines ; viral antigens</subject><ispartof>Veterinary immunology and immunopathology, 2010-08, Vol.136 (3), p.311-318</ispartof><rights>2010</rights><rights>Crown Copyright 2010. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-7e17ef09a0776aecb7dd17be6689084b77ad7aebdb07fb83588ed2655ab95ce03</citedby><cites>FETCH-LOGICAL-c483t-7e17ef09a0776aecb7dd17be6689084b77ad7aebdb07fb83588ed2655ab95ce03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0165242710000826$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20416953$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ster, Céline</creatorcontrib><creatorcontrib>Beaudoin, Frédéric</creatorcontrib><creatorcontrib>Diarra, Moussa S.</creatorcontrib><creatorcontrib>Jacques, Mario</creatorcontrib><creatorcontrib>Malouin, François</creatorcontrib><creatorcontrib>Lacasse, Pierre</creatorcontrib><title>Evaluation of some Staphylococcus aureus iron-regulated proteins as vaccine targets</title><title>Veterinary immunology and immunopathology</title><addtitle>Vet Immunol Immunopathol</addtitle><description>Staphylococcus aureus is an important pathogen that is responsible for a wide range of infections, including bovine mastitis. Previously, 54 genes from
S. aureus that were up-regulated in an iron-restricted medium and in mice were identified. Seven of those genes were selected from five iron-acquisition systems (isd, feo, sir, sst, and fhu), and the proteins were evaluated as potential vaccine targets to prevent bovine mastitis. The antigenicity of the recombinant proteins obtained with each studied gene was evaluated in rabbits and/or cattle. Immune sera were used to test the bacterial accessibility of the native proteins. All the proteins were immunogenic in rabbits or cattle. IsdH, IsdB, FeoB and SstD were expressed on the bacterial surface, with IsdB and IsdH more expressed in an iron-restricted environment. The capacity of antibodies to prevent infection was measured in a mouse mastitis model. Preincubation of
S. aureus with serum against IsdH or with the pool of sera against IsdB, SstD and FeoB led to decreased colonization of the mouse mammary glands. Lastly, cattle immunization with IsdH induced a strong and long-lasting immune response with IgG2 production. The protein IsdH appears to be a good vaccine candidate to prevent
S. aureus bovine mastitis.</description><subject>Animals</subject><subject>antibody formation</subject><subject>antigen-antibody reactions</subject><subject>antigenic variation</subject><subject>bacterial colonization</subject><subject>bacterial infections</subject><subject>bovine mastitis</subject><subject>Cattle</subject><subject>culture media</subject><subject>dairy cows</subject><subject>Disease Models, Animal</subject><subject>disease prevention</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>gene expression</subject><subject>Immune response</subject><subject>immunization</subject><subject>Immunization - methods</subject><subject>Immunization - veterinary</subject><subject>Immunoglobulin G - immunology</subject><subject>Immunoglobulin Isotypes - immunology</subject><subject>in vitro studies</subject><subject>iron</subject><subject>Iron-Regulatory Proteins - immunology</subject><subject>IsdH</subject><subject>Male</subject><subject>mammary glands</subject><subject>Mastitis</subject><subject>Mastitis, Bovine - immunology</subject><subject>Mastitis, Bovine - microbiology</subject><subject>Mastitis, Bovine - prevention & control</subject><subject>Rabbits</subject><subject>recombinant proteins</subject><subject>Recombinant Proteins - immunology</subject><subject>Staphylococcal Infections - immunology</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcal Infections - veterinary</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - immunology</subject><subject>Vaccine</subject><subject>vaccine development</subject><subject>vaccines</subject><subject>viral antigens</subject><issn>0165-2427</issn><issn>1873-2534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1O3DAURq2qqAy0b1BBdl1luI7j2NlUqhAFJCQWA2vLsW-mHiXxYDsj8fYYBViW1Sf5nvvjQ8hPCmsKtLnYrQ-Y3DiuK8hPwNY5vpAVlYKVFWf1V7LKGC-ruhLH5CTGHQDwVspv5LiCmjYtZyuyuTroYdbJ-anwfRH9iMUm6f2_58Ebb8wcCz0HzOGCn8qA23nQCW2xDz6hm3I5FgdtjJuwSDpsMcXv5KjXQ8Qfb3lKHv9ePVzelHf317eXf-5KU0uWSoFUYA-tBiEajaYT1lLRYdPIFmTdCaGt0NjZDkTfScalRFs1nOuu5QaBnZJfy9x8y9OMManRRYPDoCf0c1QSRBYBgn9KCsZaSTkXmawX0gQfY8Be7YMbdXhWFNSrd7VTi3f16l0BUzly29nbgrkb0X40vYvOwPkC9NorvQ0uqsdNnsCAyvxhqDLxeyEwKzs4DCoah5NB6wKapKx3_7_hBbacoHY</recordid><startdate>20100815</startdate><enddate>20100815</enddate><creator>Ster, Céline</creator><creator>Beaudoin, Frédéric</creator><creator>Diarra, Moussa S.</creator><creator>Jacques, Mario</creator><creator>Malouin, François</creator><creator>Lacasse, Pierre</creator><general>Elsevier B.V</general><general>Amsterdam: Elsevier</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>20100815</creationdate><title>Evaluation of some Staphylococcus aureus iron-regulated proteins as vaccine targets</title><author>Ster, Céline ; Beaudoin, Frédéric ; Diarra, Moussa S. ; Jacques, Mario ; Malouin, François ; Lacasse, Pierre</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-7e17ef09a0776aecb7dd17be6689084b77ad7aebdb07fb83588ed2655ab95ce03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>antibody formation</topic><topic>antigen-antibody reactions</topic><topic>antigenic variation</topic><topic>bacterial colonization</topic><topic>bacterial infections</topic><topic>bovine mastitis</topic><topic>Cattle</topic><topic>culture media</topic><topic>dairy cows</topic><topic>Disease Models, Animal</topic><topic>disease prevention</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>gene expression</topic><topic>Immune response</topic><topic>immunization</topic><topic>Immunization - methods</topic><topic>Immunization - veterinary</topic><topic>Immunoglobulin G - immunology</topic><topic>Immunoglobulin Isotypes - immunology</topic><topic>in vitro studies</topic><topic>iron</topic><topic>Iron-Regulatory Proteins - immunology</topic><topic>IsdH</topic><topic>Male</topic><topic>mammary glands</topic><topic>Mastitis</topic><topic>Mastitis, Bovine - immunology</topic><topic>Mastitis, Bovine - microbiology</topic><topic>Mastitis, Bovine - prevention & control</topic><topic>Rabbits</topic><topic>recombinant proteins</topic><topic>Recombinant Proteins - immunology</topic><topic>Staphylococcal Infections - immunology</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococcal Infections - veterinary</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - immunology</topic><topic>Vaccine</topic><topic>vaccine development</topic><topic>vaccines</topic><topic>viral antigens</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ster, Céline</creatorcontrib><creatorcontrib>Beaudoin, Frédéric</creatorcontrib><creatorcontrib>Diarra, Moussa S.</creatorcontrib><creatorcontrib>Jacques, Mario</creatorcontrib><creatorcontrib>Malouin, François</creatorcontrib><creatorcontrib>Lacasse, Pierre</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Veterinary immunology and immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ster, Céline</au><au>Beaudoin, Frédéric</au><au>Diarra, Moussa S.</au><au>Jacques, Mario</au><au>Malouin, François</au><au>Lacasse, Pierre</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of some Staphylococcus aureus iron-regulated proteins as vaccine targets</atitle><jtitle>Veterinary immunology and immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>2010-08-15</date><risdate>2010</risdate><volume>136</volume><issue>3</issue><spage>311</spage><epage>318</epage><pages>311-318</pages><issn>0165-2427</issn><eissn>1873-2534</eissn><abstract>Staphylococcus aureus is an important pathogen that is responsible for a wide range of infections, including bovine mastitis. Previously, 54 genes from
S. aureus that were up-regulated in an iron-restricted medium and in mice were identified. Seven of those genes were selected from five iron-acquisition systems (isd, feo, sir, sst, and fhu), and the proteins were evaluated as potential vaccine targets to prevent bovine mastitis. The antigenicity of the recombinant proteins obtained with each studied gene was evaluated in rabbits and/or cattle. Immune sera were used to test the bacterial accessibility of the native proteins. All the proteins were immunogenic in rabbits or cattle. IsdH, IsdB, FeoB and SstD were expressed on the bacterial surface, with IsdB and IsdH more expressed in an iron-restricted environment. The capacity of antibodies to prevent infection was measured in a mouse mastitis model. Preincubation of
S. aureus with serum against IsdH or with the pool of sera against IsdB, SstD and FeoB led to decreased colonization of the mouse mammary glands. Lastly, cattle immunization with IsdH induced a strong and long-lasting immune response with IgG2 production. The protein IsdH appears to be a good vaccine candidate to prevent
S. aureus bovine mastitis.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>20416953</pmid><doi>10.1016/j.vetimm.2010.03.010</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-2427 |
| ispartof | Veterinary immunology and immunopathology, 2010-08, Vol.136 (3), p.311-318 |
| issn | 0165-2427 1873-2534 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_807253075 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals antibody formation antigen-antibody reactions antigenic variation bacterial colonization bacterial infections bovine mastitis Cattle culture media dairy cows Disease Models, Animal disease prevention Female Flow Cytometry gene expression Immune response immunization Immunization - methods Immunization - veterinary Immunoglobulin G - immunology Immunoglobulin Isotypes - immunology in vitro studies iron Iron-Regulatory Proteins - immunology IsdH Male mammary glands Mastitis Mastitis, Bovine - immunology Mastitis, Bovine - microbiology Mastitis, Bovine - prevention & control Rabbits recombinant proteins Recombinant Proteins - immunology Staphylococcal Infections - immunology Staphylococcal Infections - microbiology Staphylococcal Infections - veterinary Staphylococcus aureus Staphylococcus aureus - immunology Vaccine vaccine development vaccines viral antigens |
| title | Evaluation of some Staphylococcus aureus iron-regulated proteins as vaccine targets |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T18%3A10%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20some%20Staphylococcus%20aureus%20iron-regulated%20proteins%20as%20vaccine%20targets&rft.jtitle=Veterinary%20immunology%20and%20immunopathology&rft.au=Ster,%20C%C3%A9line&rft.date=2010-08-15&rft.volume=136&rft.issue=3&rft.spage=311&rft.epage=318&rft.pages=311-318&rft.issn=0165-2427&rft.eissn=1873-2534&rft_id=info:doi/10.1016/j.vetimm.2010.03.010&rft_dat=%3Cproquest_cross%3E807253075%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=733981557&rft_id=info:pmid/20416953&rft_els_id=S0165242710000826&rfr_iscdi=true |