Amplifications of multiple regions of the HTLV-I genome from DNA of an Italian spastic paraparesis patient but not from DNA of multiple sclerosis patients
We searched for evidence of infection by the human T-cell lymphoma/leukemia virus type I (HTLV-I) in patients with multiple sclerosis (40 cases); brainstem encephalitis (1 case); Friedreich's ataxia (1 case) spactic paraparesis of unknown etiology (1 case). All patients were from the region of...
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| Veröffentlicht in: | Journal of the neurological sciences 1991-05, Vol.103 (1), p.82-89 |
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| creator | French, D. Mammarella, S. Curia, M.C. Porrini, A.M. Giampietro, A. Macor, S. Lombardi, V. Agliano', A.M. Manzari, V. Lugaresi, A. Battista, P. Frati, L. Gambi, D. Mariani-Costantini, R. |
| description | We searched for evidence of infection by the human T-cell lymphoma/leukemia virus type I (HTLV-I) in patients with multiple sclerosis (40 cases); brainstem encephalitis (1 case); Friedreich's ataxia (1 case) spactic paraparesis of unknown etiology (1 case). All patients were from the region of Abruzzo, Italy. Sera were all negative for anti-HTLV-I reactivity by the Western blotting (WB) analysis. DNAs from peripheral blood mononuclear cells were amplified using the polymerase chain reaction (PCR) technique with primers specific for the HTLV-I
gag, pol, and
env proviral regions. HTLV-I sequences were amplified only in the patient with spastic paraparesis of unknown etiology. In this case, HTLV-I infection might have been related to blood transfusions received 2 years prior to the onset of the neurologic symptoms. Members of the patient's family were negative y for HTLV-I by PCR and WB. These data indicate that HTLV-I associated myelopathy is present also in Italy, but fail to substantiate an association of HTLV-I with multiple sclerosis. |
| doi_str_mv | 10.1016/0022-510X(91)90288-I |
| format | Article |
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gag, pol, and
env proviral regions. HTLV-I sequences were amplified only in the patient with spastic paraparesis of unknown etiology. In this case, HTLV-I infection might have been related to blood transfusions received 2 years prior to the onset of the neurologic symptoms. Members of the patient's family were negative y for HTLV-I by PCR and WB. These data indicate that HTLV-I associated myelopathy is present also in Italy, but fail to substantiate an association of HTLV-I with multiple sclerosis.</description><identifier>ISSN: 0022-510X</identifier><identifier>EISSN: 1878-5883</identifier><identifier>DOI: 10.1016/0022-510X(91)90288-I</identifier><identifier>PMID: 1865236</identifier><identifier>CODEN: JNSCAG</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Adolescent ; Adult ; AIDS/HIV ; Base Sequence ; Biological and medical sciences ; Blotting, Western ; DNA, Viral - blood ; Female ; HTLV-I ; Human T-lymphotropic virus 1 - isolation & purification ; Humans ; Male ; Medical sciences ; Middle Aged ; Molecular Sequence Data ; Multiple sclerosis ; Multiple Sclerosis - microbiology ; Myelopathy ; Neurology ; Oligonucleotide Probes ; Paraparesis, Tropical Spastic - microbiology ; Polymerase Chain Reaction ; Retrovirus ; Tropical spastic paraparesis</subject><ispartof>Journal of the neurological sciences, 1991-05, Vol.103 (1), p.82-89</ispartof><rights>1991</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-ea63ef8f24e6700674816dff951b2541cc384bc8db21a9cdda0b155f4535b3403</citedby><cites>FETCH-LOGICAL-c386t-ea63ef8f24e6700674816dff951b2541cc384bc8db21a9cdda0b155f4535b3403</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0022-510X(91)90288-I$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4504863$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1865236$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>French, D.</creatorcontrib><creatorcontrib>Mammarella, S.</creatorcontrib><creatorcontrib>Curia, M.C.</creatorcontrib><creatorcontrib>Porrini, A.M.</creatorcontrib><creatorcontrib>Giampietro, A.</creatorcontrib><creatorcontrib>Macor, S.</creatorcontrib><creatorcontrib>Lombardi, V.</creatorcontrib><creatorcontrib>Agliano', A.M.</creatorcontrib><creatorcontrib>Manzari, V.</creatorcontrib><creatorcontrib>Lugaresi, A.</creatorcontrib><creatorcontrib>Battista, P.</creatorcontrib><creatorcontrib>Frati, L.</creatorcontrib><creatorcontrib>Gambi, D.</creatorcontrib><creatorcontrib>Mariani-Costantini, R.</creatorcontrib><title>Amplifications of multiple regions of the HTLV-I genome from DNA of an Italian spastic paraparesis patient but not from DNA of multiple sclerosis patients</title><title>Journal of the neurological sciences</title><addtitle>J Neurol Sci</addtitle><description>We searched for evidence of infection by the human T-cell lymphoma/leukemia virus type I (HTLV-I) in patients with multiple sclerosis (40 cases); brainstem encephalitis (1 case); Friedreich's ataxia (1 case) spactic paraparesis of unknown etiology (1 case). All patients were from the region of Abruzzo, Italy. Sera were all negative for anti-HTLV-I reactivity by the Western blotting (WB) analysis. DNAs from peripheral blood mononuclear cells were amplified using the polymerase chain reaction (PCR) technique with primers specific for the HTLV-I
gag, pol, and
env proviral regions. HTLV-I sequences were amplified only in the patient with spastic paraparesis of unknown etiology. In this case, HTLV-I infection might have been related to blood transfusions received 2 years prior to the onset of the neurologic symptoms. Members of the patient's family were negative y for HTLV-I by PCR and WB. These data indicate that HTLV-I associated myelopathy is present also in Italy, but fail to substantiate an association of HTLV-I with multiple sclerosis.</description><subject>Adolescent</subject><subject>Adult</subject><subject>AIDS/HIV</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>DNA, Viral - blood</subject><subject>Female</subject><subject>HTLV-I</subject><subject>Human T-lymphotropic virus 1 - isolation & purification</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>Multiple sclerosis</subject><subject>Multiple Sclerosis - microbiology</subject><subject>Myelopathy</subject><subject>Neurology</subject><subject>Oligonucleotide Probes</subject><subject>Paraparesis, Tropical Spastic - microbiology</subject><subject>Polymerase Chain Reaction</subject><subject>Retrovirus</subject><subject>Tropical spastic paraparesis</subject><issn>0022-510X</issn><issn>1878-5883</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc-O1SAUxonRjHdG30ATFsboogotULoxuRlHp8mNbkbjjlB6GDG0VKAmvopPK9d7verGBTmE73f-8B2EHlHyghIqXhJS1xWn5NOzjj7vSC1l1d9BGypbWXEpm7toc0Luo_OUvhBChJTdGTqjUvC6ERv0Yzst3llndHZhTjhYPK0-u8UDjnD7-y1_Bnx9s_tY9fgW5jABtjFM-PW77V7VM-6z9q7EtOiUncGLjrocSC6Ve3YwZzysGc8h_5N6apaMhxj-wtMDdM9qn-DhMV6gD2-ubi6vq937t_3ldleZRopcgRYNWGlrBqItH2yZpGK0tuN0qDmjpmBsMHIcaqo7M46aDJRzy3jDh4aR5gI9PdRdYvi6QspqcsmA93qGsCYlSVtz0vICsgNoyqApglVLdJOO3xUlar8Stfdb7f1WHVW_VqL6kvb4WH8dJhj_JB12UPQnR10no72NejYunTDGCZOiKdirAwbFi28Ookqm-GRgdBFMVmNw_5_jJwRAqcI</recordid><startdate>19910501</startdate><enddate>19910501</enddate><creator>French, D.</creator><creator>Mammarella, S.</creator><creator>Curia, M.C.</creator><creator>Porrini, A.M.</creator><creator>Giampietro, A.</creator><creator>Macor, S.</creator><creator>Lombardi, V.</creator><creator>Agliano', A.M.</creator><creator>Manzari, V.</creator><creator>Lugaresi, A.</creator><creator>Battista, P.</creator><creator>Frati, L.</creator><creator>Gambi, D.</creator><creator>Mariani-Costantini, R.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19910501</creationdate><title>Amplifications of multiple regions of the HTLV-I genome from DNA of an Italian spastic paraparesis patient but not from DNA of multiple sclerosis patients</title><author>French, D. ; Mammarella, S. ; Curia, M.C. ; Porrini, A.M. ; Giampietro, A. ; Macor, S. ; Lombardi, V. ; Agliano', A.M. ; Manzari, V. ; Lugaresi, A. ; Battista, P. ; Frati, L. ; Gambi, D. ; Mariani-Costantini, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-ea63ef8f24e6700674816dff951b2541cc384bc8db21a9cdda0b155f4535b3403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>AIDS/HIV</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>DNA, Viral - blood</topic><topic>Female</topic><topic>HTLV-I</topic><topic>Human T-lymphotropic virus 1 - isolation & purification</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Molecular Sequence Data</topic><topic>Multiple sclerosis</topic><topic>Multiple Sclerosis - microbiology</topic><topic>Myelopathy</topic><topic>Neurology</topic><topic>Oligonucleotide Probes</topic><topic>Paraparesis, Tropical Spastic - microbiology</topic><topic>Polymerase Chain Reaction</topic><topic>Retrovirus</topic><topic>Tropical spastic paraparesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>French, D.</creatorcontrib><creatorcontrib>Mammarella, S.</creatorcontrib><creatorcontrib>Curia, M.C.</creatorcontrib><creatorcontrib>Porrini, A.M.</creatorcontrib><creatorcontrib>Giampietro, A.</creatorcontrib><creatorcontrib>Macor, S.</creatorcontrib><creatorcontrib>Lombardi, V.</creatorcontrib><creatorcontrib>Agliano', A.M.</creatorcontrib><creatorcontrib>Manzari, V.</creatorcontrib><creatorcontrib>Lugaresi, A.</creatorcontrib><creatorcontrib>Battista, P.</creatorcontrib><creatorcontrib>Frati, L.</creatorcontrib><creatorcontrib>Gambi, D.</creatorcontrib><creatorcontrib>Mariani-Costantini, R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the neurological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>French, D.</au><au>Mammarella, S.</au><au>Curia, M.C.</au><au>Porrini, A.M.</au><au>Giampietro, A.</au><au>Macor, S.</au><au>Lombardi, V.</au><au>Agliano', A.M.</au><au>Manzari, V.</au><au>Lugaresi, A.</au><au>Battista, P.</au><au>Frati, L.</au><au>Gambi, D.</au><au>Mariani-Costantini, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amplifications of multiple regions of the HTLV-I genome from DNA of an Italian spastic paraparesis patient but not from DNA of multiple sclerosis patients</atitle><jtitle>Journal of the neurological sciences</jtitle><addtitle>J Neurol Sci</addtitle><date>1991-05-01</date><risdate>1991</risdate><volume>103</volume><issue>1</issue><spage>82</spage><epage>89</epage><pages>82-89</pages><issn>0022-510X</issn><eissn>1878-5883</eissn><coden>JNSCAG</coden><abstract>We searched for evidence of infection by the human T-cell lymphoma/leukemia virus type I (HTLV-I) in patients with multiple sclerosis (40 cases); brainstem encephalitis (1 case); Friedreich's ataxia (1 case) spactic paraparesis of unknown etiology (1 case). All patients were from the region of Abruzzo, Italy. Sera were all negative for anti-HTLV-I reactivity by the Western blotting (WB) analysis. DNAs from peripheral blood mononuclear cells were amplified using the polymerase chain reaction (PCR) technique with primers specific for the HTLV-I
gag, pol, and
env proviral regions. HTLV-I sequences were amplified only in the patient with spastic paraparesis of unknown etiology. In this case, HTLV-I infection might have been related to blood transfusions received 2 years prior to the onset of the neurologic symptoms. Members of the patient's family were negative y for HTLV-I by PCR and WB. These data indicate that HTLV-I associated myelopathy is present also in Italy, but fail to substantiate an association of HTLV-I with multiple sclerosis.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>1865236</pmid><doi>10.1016/0022-510X(91)90288-I</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-510X |
| ispartof | Journal of the neurological sciences, 1991-05, Vol.103 (1), p.82-89 |
| issn | 0022-510X 1878-5883 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adolescent Adult AIDS/HIV Base Sequence Biological and medical sciences Blotting, Western DNA, Viral - blood Female HTLV-I Human T-lymphotropic virus 1 - isolation & purification Humans Male Medical sciences Middle Aged Molecular Sequence Data Multiple sclerosis Multiple Sclerosis - microbiology Myelopathy Neurology Oligonucleotide Probes Paraparesis, Tropical Spastic - microbiology Polymerase Chain Reaction Retrovirus Tropical spastic paraparesis |
| title | Amplifications of multiple regions of the HTLV-I genome from DNA of an Italian spastic paraparesis patient but not from DNA of multiple sclerosis patients |
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