Influence of ELISA conditions on detection of serological relationships among luteoviruses
Three variations in the ELISA procedure were used in an attempt to understand the basis for serological relationships among three isolates of barley yellow dwarf virus (BYDV-RPV-I from Illinois, BYDV-RPV-N from New York and BYDV-PAV from Illinois), beet western yellows virus (BWYV) and soybean dwarf...
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| Veröffentlicht in: | Journal of virological methods 1991-02, Vol.31 (2), p.263-271 |
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| container_title | Journal of virological methods |
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| creator | Murphy, John F. D'Arcy, Cleora J. |
| description | Three variations in the ELISA procedure were used in an attempt to understand the basis for serological relationships among three isolates of barley yellow dwarf virus (BYDV-RPV-I from Illinois, BYDV-RPV-N from New York and BYDV-PAV from Illinois), beet western yellows virus (BWYV) and soybean dwarf virus (SDV). Detection of serological relationships was dependent on the state of the virus particle (e.g. dissociated or intact) and the method of detection (e.g. direct or indirect). In indirect ELISA, where virus particles were dissociated due to incubation in a high pH buffer, all five virus isolates were serologically related. In double antibody sandwich (DAS) ELISA, identification of serological relationships was based on detection of epitopes associated with intact virus particles, which resulted the detection of fewer serological relationships. Direct ELISA showed, depending on the Ig, that the state of the virus particle and/or the method of detection did effect the ability to detect some serological relationships. |
| doi_str_mv | 10.1016/0166-0934(91)90164-U |
| format | Article |
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Detection of serological relationships was dependent on the state of the virus particle (e.g. dissociated or intact) and the method of detection (e.g. direct or indirect). In indirect ELISA, where virus particles were dissociated due to incubation in a high pH buffer, all five virus isolates were serologically related. In double antibody sandwich (DAS) ELISA, identification of serological relationships was based on detection of epitopes associated with intact virus particles, which resulted the detection of fewer serological relationships. Direct ELISA showed, depending on the Ig, that the state of the virus particle and/or the method of detection did effect the ability to detect some serological relationships.</description><identifier>ISSN: 0166-0934</identifier><identifier>EISSN: 1879-0984</identifier><identifier>DOI: 10.1016/0166-0934(91)90164-U</identifier><identifier>PMID: 1713915</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; barley yellow dwarf luteovirus ; Beet western yellows virus ; direct elisa ; double antibody sandwich elisa ; ELISA ; Enzyme-Linked Immunosorbent Assay ; Epitopes ; immunoglobulins ; indirect elisa ; Luteovirus ; particles ; Plant Viruses - isolation & purification ; Rabbits ; serological relationships ; Soybean dwarf virus ; Virion - immunology ; Virion - isolation & purification</subject><ispartof>Journal of virological methods, 1991-02, Vol.31 (2), p.263-271</ispartof><rights>1991</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-5adef4c1377761553739c30d014b51bc77ef9cb1c19a0125318ebf2a4edaa2a03</citedby><cites>FETCH-LOGICAL-c412t-5adef4c1377761553739c30d014b51bc77ef9cb1c19a0125318ebf2a4edaa2a03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0166-0934(91)90164-U$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1713915$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Murphy, John F.</creatorcontrib><creatorcontrib>D'Arcy, Cleora J.</creatorcontrib><title>Influence of ELISA conditions on detection of serological relationships among luteoviruses</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>Three variations in the ELISA procedure were used in an attempt to understand the basis for serological relationships among three isolates of barley yellow dwarf virus (BYDV-RPV-I from Illinois, BYDV-RPV-N from New York and BYDV-PAV from Illinois), beet western yellows virus (BWYV) and soybean dwarf virus (SDV). Detection of serological relationships was dependent on the state of the virus particle (e.g. dissociated or intact) and the method of detection (e.g. direct or indirect). In indirect ELISA, where virus particles were dissociated due to incubation in a high pH buffer, all five virus isolates were serologically related. In double antibody sandwich (DAS) ELISA, identification of serological relationships was based on detection of epitopes associated with intact virus particles, which resulted the detection of fewer serological relationships. Direct ELISA showed, depending on the Ig, that the state of the virus particle and/or the method of detection did effect the ability to detect some serological relationships.</description><subject>Animals</subject><subject>barley yellow dwarf luteovirus</subject><subject>Beet western yellows virus</subject><subject>direct elisa</subject><subject>double antibody sandwich elisa</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epitopes</subject><subject>immunoglobulins</subject><subject>indirect elisa</subject><subject>Luteovirus</subject><subject>particles</subject><subject>Plant Viruses - isolation & purification</subject><subject>Rabbits</subject><subject>serological relationships</subject><subject>Soybean dwarf virus</subject><subject>Virion - immunology</subject><subject>Virion - isolation & purification</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFq3DAQQEVo2G43-YOW-hTag1ONJK-sS2AJm2RhIYd0L7kIWR5vVbzWVrID-fvIcWhvzUGImXkzDG8I-Qz0Eigsf6S3zKni4puC7ypFIt-dkDmUUqV0KT6Q-V_kI_kU429KaSE5n5EZSOAKijl53HRNO2BnMfNNtt5uHlaZ9V3teue7mPkuq7FHO0YjEDH41u-dNW0WsDWv1C93jJk5-G6ftUOP_smFIWI8I6eNaSOev_0LsrtZ_7y-y7f3t5vr1Ta3AlifF6bGRljgUsolFAWXXFlOawqiKqCyUmKjbAUWlKHACg4lVg0zAmtjmKF8QS6mucfg_wwYe31w0WLbmg79EHVJJWOKle-CUJQqbQAJFBNog48xYKOPwR1MeNZA9ehej2L1KFYr0K_u9S61fXmbP1QHrP81TbJT_etUb4zXZh9c1LsHRoFTkEIwKhNxNRGYfD05DDpaNx6ndiEdQdfe_X-FF0uonRQ</recordid><startdate>19910201</startdate><enddate>19910201</enddate><creator>Murphy, John F.</creator><creator>D'Arcy, Cleora J.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19910201</creationdate><title>Influence of ELISA conditions on detection of serological relationships among luteoviruses</title><author>Murphy, John F. ; D'Arcy, Cleora J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-5adef4c1377761553739c30d014b51bc77ef9cb1c19a0125318ebf2a4edaa2a03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>barley yellow dwarf luteovirus</topic><topic>Beet western yellows virus</topic><topic>direct elisa</topic><topic>double antibody sandwich elisa</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epitopes</topic><topic>immunoglobulins</topic><topic>indirect elisa</topic><topic>Luteovirus</topic><topic>particles</topic><topic>Plant Viruses - isolation & purification</topic><topic>Rabbits</topic><topic>serological relationships</topic><topic>Soybean dwarf virus</topic><topic>Virion - immunology</topic><topic>Virion - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Murphy, John F.</creatorcontrib><creatorcontrib>D'Arcy, Cleora J.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Murphy, John F.</au><au>D'Arcy, Cleora J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of ELISA conditions on detection of serological relationships among luteoviruses</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>1991-02-01</date><risdate>1991</risdate><volume>31</volume><issue>2</issue><spage>263</spage><epage>271</epage><pages>263-271</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><abstract>Three variations in the ELISA procedure were used in an attempt to understand the basis for serological relationships among three isolates of barley yellow dwarf virus (BYDV-RPV-I from Illinois, BYDV-RPV-N from New York and BYDV-PAV from Illinois), beet western yellows virus (BWYV) and soybean dwarf virus (SDV). 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| fulltext | fulltext |
| identifier | ISSN: 0166-0934 |
| ispartof | Journal of virological methods, 1991-02, Vol.31 (2), p.263-271 |
| issn | 0166-0934 1879-0984 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80722928 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals barley yellow dwarf luteovirus Beet western yellows virus direct elisa double antibody sandwich elisa ELISA Enzyme-Linked Immunosorbent Assay Epitopes immunoglobulins indirect elisa Luteovirus particles Plant Viruses - isolation & purification Rabbits serological relationships Soybean dwarf virus Virion - immunology Virion - isolation & purification |
| title | Influence of ELISA conditions on detection of serological relationships among luteoviruses |
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