Detection of mercuric ions in water by ELISA with a mercury-specific antibody
An immunoassay that detects mercuric ions in water at concentrations of 0.5 ppb and above is described. The assay utilizes a monoclonal antibody that binds specifically to mercuric ions immobilized in wells of microtiter plates. Within the range of 0.5–10 ppb mercury, the absorbance in the enzyme-li...
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| Veröffentlicht in: | Analytical biochemistry 1991-05, Vol.194 (2), p.381-387 |
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| container_title | Analytical biochemistry |
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| creator | Wylie, Dwane E. Carlson, Larry D. Carlson, Randy Wagner, Fred W. Schuster, Sheldon M. |
| description | An immunoassay that detects mercuric ions in water at concentrations of 0.5 ppb and above is described. The assay utilizes a monoclonal antibody that binds specifically to mercuric ions immobilized in wells of microtiter plates. Within the range of 0.5–10 ppb mercury, the absorbance in the enzyme-linked immunosorbent assay (ELISA) is linear to the log of the mercuric ion concentration. The quantitation of mercury by ELISA correlates closely with results from cold-vapor atomic absorption. Other divalent metal cations do not interfere with the assay, although there is interference in the presence of 1 m
m chloride ions. The optimum pH for mercury detection is 7.0, although 2 ppb mercury can be detected over a wide pH range. The assay is as sensitive as cold-vapor atomic absorption for mercury detection and can be performed with only 100 μl of sample. |
| doi_str_mv | 10.1016/0003-2697(91)90245-O |
| format | Article |
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m chloride ions. The optimum pH for mercury detection is 7.0, although 2 ppb mercury can be detected over a wide pH range. The assay is as sensitive as cold-vapor atomic absorption for mercury detection and can be performed with only 100 μl of sample.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - biosynthesis</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Chlorides</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Mercury - analysis</subject><subject>Mercury - immunology</subject><subject>Methods. Procedures. Technologies</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Others</subject><subject>Spectrophotometry, Atomic</subject><subject>Various methods and equipments</subject><subject>Water - chemistry</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFKAzEQhoMotVbfQCEXRQ-ryW4221yEUqsWKj2o55CdTDDS7tZka-nbu7XF3jwNw3z_zPARcs7ZLWdc3jHGsiSVqrhW_EaxVOTJ9IB0OVMyYRlTh6T7hxyTkxg_GeNc5LJDOrwvUyVYl7w8YIPQ-LqitaNzDLAMHmjbR-orujINBlqu6Wgyfh3QlW8-qNlh6yQuELxrcVM1vqzt-pQcOTOLeLarPfL-OHobPieT6dN4OJgkINKsScC5LANuiwKsyoTMrbJpiVLloi-hRAvWOsgNCG5KxguRc-tyIUpRMikgy3rkart3EeqvJcZGz30EnM1MhfUy6j4ruBJKtqDYghDqGAM6vQh-bsJac6Y3FvVGkd4o0orrX4t62sYudvuX5RztPrTV1s4vd3MTwcxcMBX4uMdUkfZVujl_v-WwlfHtMegIHitA60NrXdva___ID6p8jh0</recordid><startdate>19910501</startdate><enddate>19910501</enddate><creator>Wylie, Dwane E.</creator><creator>Carlson, Larry D.</creator><creator>Carlson, Randy</creator><creator>Wagner, Fred W.</creator><creator>Schuster, Sheldon M.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19910501</creationdate><title>Detection of mercuric ions in water by ELISA with a mercury-specific antibody</title><author>Wylie, Dwane E. ; Carlson, Larry D. ; Carlson, Randy ; Wagner, Fred W. ; Schuster, Sheldon M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-cff33c1d77cd93465d9d2be695486cbedcddfc5ac41ab017451df544b4b064c33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - biosynthesis</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Chlorides</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Mercury - analysis</topic><topic>Mercury - immunology</topic><topic>Methods. Procedures. Technologies</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Others</topic><topic>Spectrophotometry, Atomic</topic><topic>Various methods and equipments</topic><topic>Water - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wylie, Dwane E.</creatorcontrib><creatorcontrib>Carlson, Larry D.</creatorcontrib><creatorcontrib>Carlson, Randy</creatorcontrib><creatorcontrib>Wagner, Fred W.</creatorcontrib><creatorcontrib>Schuster, Sheldon M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wylie, Dwane E.</au><au>Carlson, Larry D.</au><au>Carlson, Randy</au><au>Wagner, Fred W.</au><au>Schuster, Sheldon M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of mercuric ions in water by ELISA with a mercury-specific antibody</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1991-05-01</date><risdate>1991</risdate><volume>194</volume><issue>2</issue><spage>381</spage><epage>387</epage><pages>381-387</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><coden>ANBCA2</coden><abstract>An immunoassay that detects mercuric ions in water at concentrations of 0.5 ppb and above is described. 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m chloride ions. The optimum pH for mercury detection is 7.0, although 2 ppb mercury can be detected over a wide pH range. The assay is as sensitive as cold-vapor atomic absorption for mercury detection and can be performed with only 100 μl of sample.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1862940</pmid><doi>10.1016/0003-2697(91)90245-O</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Analytical biochemistry, 1991-05, Vol.194 (2), p.381-387 |
| issn | 0003-2697 1096-0309 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - immunology Biological and medical sciences Biotechnology Chlorides Enzyme-Linked Immunosorbent Assay - methods Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Mercury - analysis Mercury - immunology Methods. Procedures. Technologies Mice Mice, Inbred BALB C Others Spectrophotometry, Atomic Various methods and equipments Water - chemistry |
| title | Detection of mercuric ions in water by ELISA with a mercury-specific antibody |
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