Pulsed field gel electrophoresis as a new epidemiological tool for monitoring methicillin-resistant Staphylococcus aureus in an intensive care unit
Pulsed field gel electrophoresis (PFGE) of bacterial DNA was used in a 1-month epidemiological study of methicillin-resistant Staphylococcus aureus (MRSA) in a 15-bed Intensive Care Unit (ICU). Patient and hospital staff carriage as well as distribution of MRSA in the ICU environment were investigat...
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| Veröffentlicht in: | The Journal of hospital infection 1991, Vol.17 (4), p.255-269 |
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| container_title | The Journal of hospital infection |
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| creator | Prévost, G. Pottecher, B. Dahlet, M. Bientz, M. Mantz, J.M. Piémont, Y. |
| description | Pulsed field gel electrophoresis (PFGE) of bacterial DNA was used in a 1-month epidemiological study of methicillin-resistant
Staphylococcus aureus (MRSA) in a 15-bed Intensive Care Unit (ICU). Patient and hospital staff carriage as well as distribution of MRSA in the ICU environment were investigated, and a total of 3802 samples produced 175 isolates. The stability and the reproducibility of the PFGE method were satisfactory. Moreover, the plasmid content of the strains so far examined had no influence on the PFGE profiles of the MRSA strains. The polymorphic profiles observed also account for the use of this method as an epidemiological tool for investigating MRSA. Among 30 patients who stayed more than 4 days in the unit, PFGE analysis showed 11 episodes of colonization in nine patients, whereas lysotyping and plasmid DNA analysis demonstrated only eight and seven such episodes in the same patients, respectively. The combination of PFGE with lysotyping and plasmid analysis may provide a greater discriminatory capacity between MRSA isolates. |
| doi_str_mv | 10.1016/0195-6701(91)90270-I |
| format | Article |
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Staphylococcus aureus (MRSA) in a 15-bed Intensive Care Unit (ICU). Patient and hospital staff carriage as well as distribution of MRSA in the ICU environment were investigated, and a total of 3802 samples produced 175 isolates. The stability and the reproducibility of the PFGE method were satisfactory. Moreover, the plasmid content of the strains so far examined had no influence on the PFGE profiles of the MRSA strains. The polymorphic profiles observed also account for the use of this method as an epidemiological tool for investigating MRSA. Among 30 patients who stayed more than 4 days in the unit, PFGE analysis showed 11 episodes of colonization in nine patients, whereas lysotyping and plasmid DNA analysis demonstrated only eight and seven such episodes in the same patients, respectively. The combination of PFGE with lysotyping and plasmid analysis may provide a greater discriminatory capacity between MRSA isolates.</description><identifier>ISSN: 0195-6701</identifier><identifier>EISSN: 1532-2939</identifier><identifier>DOI: 10.1016/0195-6701(91)90270-I</identifier><identifier>PMID: 1677651</identifier><language>eng</language><publisher>Kent: Elsevier Ltd</publisher><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Bacteriophage Typing ; Biological and medical sciences ; DNA, Bacterial - analysis ; Electrophoresis, Agar Gel - methods ; Emergency and intensive care: infection, septic shock ; epidemiological study ; Humans ; Intensive care medicine ; intensive care unit ; Intensive Care Units ; lysotyping ; Medical sciences ; Methicillin Resistance ; Methicillin-resistant Staphylococcus aureus ; plasmid DNA ; Plasmids - genetics ; pulsed field gel electrophoresis ; Reproducibility of Results ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - genetics</subject><ispartof>The Journal of hospital infection, 1991, Vol.17 (4), p.255-269</ispartof><rights>1991</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c397t-e4d9b8d0cb90f7f5954b84937c115f2b027499ad4a79cd9b7330c138658d9e753</citedby><cites>FETCH-LOGICAL-c397t-e4d9b8d0cb90f7f5954b84937c115f2b027499ad4a79cd9b7330c138658d9e753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/019567019190270I$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19725024$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1677651$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Prévost, G.</creatorcontrib><creatorcontrib>Pottecher, B.</creatorcontrib><creatorcontrib>Dahlet, M.</creatorcontrib><creatorcontrib>Bientz, M.</creatorcontrib><creatorcontrib>Mantz, J.M.</creatorcontrib><creatorcontrib>Piémont, Y.</creatorcontrib><title>Pulsed field gel electrophoresis as a new epidemiological tool for monitoring methicillin-resistant Staphylococcus aureus in an intensive care unit</title><title>The Journal of hospital infection</title><addtitle>J Hosp Infect</addtitle><description>Pulsed field gel electrophoresis (PFGE) of bacterial DNA was used in a 1-month epidemiological study of methicillin-resistant
Staphylococcus aureus (MRSA) in a 15-bed Intensive Care Unit (ICU). Patient and hospital staff carriage as well as distribution of MRSA in the ICU environment were investigated, and a total of 3802 samples produced 175 isolates. The stability and the reproducibility of the PFGE method were satisfactory. Moreover, the plasmid content of the strains so far examined had no influence on the PFGE profiles of the MRSA strains. The polymorphic profiles observed also account for the use of this method as an epidemiological tool for investigating MRSA. Among 30 patients who stayed more than 4 days in the unit, PFGE analysis showed 11 episodes of colonization in nine patients, whereas lysotyping and plasmid DNA analysis demonstrated only eight and seven such episodes in the same patients, respectively. The combination of PFGE with lysotyping and plasmid analysis may provide a greater discriminatory capacity between MRSA isolates.</description><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Bacteriophage Typing</subject><subject>Biological and medical sciences</subject><subject>DNA, Bacterial - analysis</subject><subject>Electrophoresis, Agar Gel - methods</subject><subject>Emergency and intensive care: infection, septic shock</subject><subject>epidemiological study</subject><subject>Humans</subject><subject>Intensive care medicine</subject><subject>intensive care unit</subject><subject>Intensive Care Units</subject><subject>lysotyping</subject><subject>Medical sciences</subject><subject>Methicillin Resistance</subject><subject>Methicillin-resistant Staphylococcus aureus</subject><subject>plasmid DNA</subject><subject>Plasmids - genetics</subject><subject>pulsed field gel electrophoresis</subject><subject>Reproducibility of Results</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - genetics</subject><issn>0195-6701</issn><issn>1532-2939</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kd2KFDEQhYMo67j6Bgq5UfSiNenudCY3giz-DCwoqNchnVTPlKSTNkmv7HP4wmacwb0TihSkvjoU5xDylLPXnPHhDeNKNINk_KXirxRrJWt298iGi65tWtWp-2TzD3lIHuX8gzFW_8UFueCDlIPgG_L7y-ozODoheEf34Cl4sCXF5RATZMzU1KIBflFY0MGM0cc9WuNpidHTKSY6x4AlJgx7OkM5oEXvMTR_14sJhX4tZjnc-mijtWuVWxPUhoGaUN8CIeMNUGsS0LVKPSYPJlOvenLul-T7h_ffrj41158_7q7eXTe2U7I00Ds1bh2zo2KTnIQS_bjtVSct52Jqx-pIr5RxvZHKVlR2HbO82w5i6xRI0V2SFyfdJcWfK-SiZ8wWvDcB4pr1lkmmmJAV7E-gTTHnBJNeEs4m3WrO9DELfTRaH43WqtYxC72ra8_O-us4g7tbOplf58_Pc5OroVMywWK-w5RsBWv7yr09cVDNuEFIOluEYMFhqllpF_H_h_wBZJCo-Q</recordid><startdate>1991</startdate><enddate>1991</enddate><creator>Prévost, G.</creator><creator>Pottecher, B.</creator><creator>Dahlet, M.</creator><creator>Bientz, M.</creator><creator>Mantz, J.M.</creator><creator>Piémont, Y.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1991</creationdate><title>Pulsed field gel electrophoresis as a new epidemiological tool for monitoring methicillin-resistant Staphylococcus aureus in an intensive care unit</title><author>Prévost, G. ; Pottecher, B. ; Dahlet, M. ; Bientz, M. ; Mantz, J.M. ; Piémont, Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-e4d9b8d0cb90f7f5954b84937c115f2b027499ad4a79cd9b7330c138658d9e753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Bacteriophage Typing</topic><topic>Biological and medical sciences</topic><topic>DNA, Bacterial - analysis</topic><topic>Electrophoresis, Agar Gel - methods</topic><topic>Emergency and intensive care: infection, septic shock</topic><topic>epidemiological study</topic><topic>Humans</topic><topic>Intensive care medicine</topic><topic>intensive care unit</topic><topic>Intensive Care Units</topic><topic>lysotyping</topic><topic>Medical sciences</topic><topic>Methicillin Resistance</topic><topic>Methicillin-resistant Staphylococcus aureus</topic><topic>plasmid DNA</topic><topic>Plasmids - genetics</topic><topic>pulsed field gel electrophoresis</topic><topic>Reproducibility of Results</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Prévost, G.</creatorcontrib><creatorcontrib>Pottecher, B.</creatorcontrib><creatorcontrib>Dahlet, M.</creatorcontrib><creatorcontrib>Bientz, M.</creatorcontrib><creatorcontrib>Mantz, J.M.</creatorcontrib><creatorcontrib>Piémont, Y.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of hospital infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Prévost, G.</au><au>Pottecher, B.</au><au>Dahlet, M.</au><au>Bientz, M.</au><au>Mantz, J.M.</au><au>Piémont, Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pulsed field gel electrophoresis as a new epidemiological tool for monitoring methicillin-resistant Staphylococcus aureus in an intensive care unit</atitle><jtitle>The Journal of hospital infection</jtitle><addtitle>J Hosp Infect</addtitle><date>1991</date><risdate>1991</risdate><volume>17</volume><issue>4</issue><spage>255</spage><epage>269</epage><pages>255-269</pages><issn>0195-6701</issn><eissn>1532-2939</eissn><abstract>Pulsed field gel electrophoresis (PFGE) of bacterial DNA was used in a 1-month epidemiological study of methicillin-resistant
Staphylococcus aureus (MRSA) in a 15-bed Intensive Care Unit (ICU). Patient and hospital staff carriage as well as distribution of MRSA in the ICU environment were investigated, and a total of 3802 samples produced 175 isolates. The stability and the reproducibility of the PFGE method were satisfactory. Moreover, the plasmid content of the strains so far examined had no influence on the PFGE profiles of the MRSA strains. The polymorphic profiles observed also account for the use of this method as an epidemiological tool for investigating MRSA. Among 30 patients who stayed more than 4 days in the unit, PFGE analysis showed 11 episodes of colonization in nine patients, whereas lysotyping and plasmid DNA analysis demonstrated only eight and seven such episodes in the same patients, respectively. The combination of PFGE with lysotyping and plasmid analysis may provide a greater discriminatory capacity between MRSA isolates.</abstract><cop>Kent</cop><pub>Elsevier Ltd</pub><pmid>1677651</pmid><doi>10.1016/0195-6701(91)90270-I</doi><tpages>15</tpages></addata></record> |
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| ispartof | The Journal of hospital infection, 1991, Vol.17 (4), p.255-269 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Bacteriophage Typing Biological and medical sciences DNA, Bacterial - analysis Electrophoresis, Agar Gel - methods Emergency and intensive care: infection, septic shock epidemiological study Humans Intensive care medicine intensive care unit Intensive Care Units lysotyping Medical sciences Methicillin Resistance Methicillin-resistant Staphylococcus aureus plasmid DNA Plasmids - genetics pulsed field gel electrophoresis Reproducibility of Results Staphylococcus aureus - drug effects Staphylococcus aureus - genetics |
| title | Pulsed field gel electrophoresis as a new epidemiological tool for monitoring methicillin-resistant Staphylococcus aureus in an intensive care unit |
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