Mutual arrangement of histone H1 molecules in extended chromatin: Chymotryptic digestion of cross-linked H1 histone dimers
Mutual arrangement of histone H1 molecules in chromatin extended in low salt—EDTA buffer and additionally in the presence of urea was studied by means of reversible cross-linking combined with chymotryptic digestion. In the chromatins tested, the chymotryptic halves of H1 were cross-linked in all po...
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| Veröffentlicht in: | FEBS letters 1983-10, Vol.163 (1), p.66-68 |
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| container_end_page | 68 |
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| container_issue | 1 |
| container_start_page | 66 |
| container_title | FEBS letters |
| container_volume | 163 |
| creator | Nikolaev, Lev G. Glotov, Boris O. Dashkevich, Viktor K. Barbashov, Sergei F. Severin, Eugenii S. |
| description | Mutual arrangement of histone H1 molecules in chromatin extended in low salt—EDTA buffer and additionally in the presence of urea was studied by means of reversible cross-linking combined with chymotryptic digestion. In the chromatins tested, the chymotryptic halves of H1 were cross-linked in all possible combinations; i.e., C—C, C—N and N—N. The results imply that the mutual arrangement of H1 histones is determined by the structure of extended nucleosomal chain, rather than chromatin superstructure. |
| doi_str_mv | 10.1016/0014-5793(83)81164-8 |
| format | Article |
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Psychology</subject><subject>Histone H1 arrangement</subject><subject>Histones - analysis</subject><subject>Macromolecular Substances</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Weight</subject><subject>Protein cross-linking</subject><subject>Thymus Gland - analysis</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-LFDEQxYMo6-zoN1DIQWQ9tCadP5PsQZBBXWHFi55DOqneiXanxyQtjp_etDPOUSGQFPXqR-U9hJ5Q8pISKl8RQnkjNppdKfZCUSp5o-6hFVUb1jAu1X20Okseosucv5JaK6ov0IWUrZKarNCvj3OZ7YBtSjbewQix4KnHu5DLFAHfUDxOA7h5gIxDxPCzQPTgsdulabQlxGu83R3GqaTDvgSHfbiDXMIUF4pLU87NEOK3OlFRf6k-jJDyI_Sgt0OGx6d7jb68e_t5e9Pcfnr_YfvmtnFMytLoDhQnUrjWEUG86jvGeMeBKmCWcSZ0p4hoBehOeNpq6Sxpue9pZ3VnFWVr9PzI3afp-1y3M2PIDobBRpjmbBTZECGl-K-Qsg3fcCGrkB-Ffz6YoDf7FEabDoYSs2RjFuPNYrxR9SzZ1McaPT3x524Efx46hVH7z059m50d-pqIC_ks05xzXZFr9Poog2rajwDJZBcgOvAhgSvGT-Hfe_wGX0KrnQ</recordid><startdate>19831031</startdate><enddate>19831031</enddate><creator>Nikolaev, Lev G.</creator><creator>Glotov, Boris O.</creator><creator>Dashkevich, Viktor K.</creator><creator>Barbashov, Sergei F.</creator><creator>Severin, Eugenii S.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19831031</creationdate><title>Mutual arrangement of histone H1 molecules in extended chromatin: Chymotryptic digestion of cross-linked H1 histone dimers</title><author>Nikolaev, Lev G. ; Glotov, Boris O. ; Dashkevich, Viktor K. ; Barbashov, Sergei F. ; Severin, Eugenii S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c366t-9be84065c2c050d8fb334b4e18e3a34359b80525e9b5d1296ca024df1ba9ba813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Cell Nucleus - ultrastructure</topic><topic>Chromatin - ultrastructure</topic><topic>Chromatin structure</topic><topic>Chromatin. 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| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1983-10, Vol.163 (1), p.66-68 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80705665 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Biological and medical sciences Cattle Cell Nucleus - ultrastructure Chromatin - ultrastructure Chromatin structure Chromatin. Chromosome Chymotrypsin Chymotryptic digestion Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Histone H1 arrangement Histones - analysis Macromolecular Substances Molecular and cellular biology Molecular genetics Molecular Weight Protein cross-linking Thymus Gland - analysis |
| title | Mutual arrangement of histone H1 molecules in extended chromatin: Chymotryptic digestion of cross-linked H1 histone dimers |
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