Identification and characterization of important allergens from mugwort pollen by IEF, SDS-PAGE and immunoblotting

Mugwort ( Artemisia vulgaris L.) pollen allergens, separated by SDS-PAGE or IEF, were identified after transfer to NCM by incubation with a panel of sera from 16 patients with clinical mugwort pollen allergy, followed by [ 125I]anti-IgE and autoradiography. Of the at least 23 components separated by...

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Veröffentlicht in:	Molecular immunology 1991-07, Vol.28 (7), p.733-742
Hauptverfasser:	Nilsen, B.M., Grimsøen, A., Paulsen, B.Smestad
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Electrophoresis, Polyacrylamide Gel Humans Hypersensitivity - immunology Immunoblotting Immunoglobulin E - immunology Isoelectric Focusing Isoelectric Point Molecular Weight Pollen - analysis Pollen - immunology Rabbits
Online-Zugang:	Volltext
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