Application of Hepatitis B Virus (HBV) DNA Sequence Polymorphisms to the Study of HBV Transmission
Short sequencesin hypervariableregions of the hepatitis Bvirus (HBV)genome can beused to identify different strains, providing a novel approach to the study of HBV transmission. The nucleotide sequence in positions 2551–2650 (1: EcoRI site) was determined for serum HBV DNA from 96 Chinese children l...
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| Veröffentlicht in: | The Journal of infectious diseases 1991-08, Vol.164 (2), p.284-288 |
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| container_title | The Journal of infectious diseases |
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| creator | Lin, Hsiang Ju Lai, Ching-lung Lauder, I. J. Wu, Pui-chee Lau, Theodore K. Fong, Ming-wei |
| description | Short sequencesin hypervariableregions of the hepatitis Bvirus (HBV)genome can beused to identify different strains, providing a novel approach to the study of HBV transmission. The nucleotide sequence in positions 2551–2650 (1: EcoRI site) was determined for serum HBV DNA from 96 Chinese children living in Hong Kong and from 38 of their parents. HBV DNA was extracted and sequenced after amplification with the polymerase chain reaction, using as primers oligonucleotides corresponding to two conserved sequences. Among 82 unrelated children, 32 HBV DNA variants were present. One sequence was present in 33 children and 31 variants were found among the other 49. Siblings within each of nine families had the same variant; in three families siblings had different variants. Six of the eight fathers and 28 of the 30 mothers had HBV DNA sequences identical to those of their offspring. A total of 34 variants were found among the 134 individuals. The hypothesis of random assortment of sequences in parents and children was rejected (P < .00005). Thus, this newapproach proves the occurrence of intrafamilial transmission of HBV among Chinese. |
| doi_str_mv | 10.1093/infdis/164.2.284 |
| format | Article |
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J. ; Wu, Pui-chee ; Lau, Theodore K. ; Fong, Ming-wei</creator><creatorcontrib>Lin, Hsiang Ju ; Lai, Ching-lung ; Lauder, I. J. ; Wu, Pui-chee ; Lau, Theodore K. ; Fong, Ming-wei</creatorcontrib><description>Short sequencesin hypervariableregions of the hepatitis Bvirus (HBV)genome can beused to identify different strains, providing a novel approach to the study of HBV transmission. The nucleotide sequence in positions 2551–2650 (1: EcoRI site) was determined for serum HBV DNA from 96 Chinese children living in Hong Kong and from 38 of their parents. HBV DNA was extracted and sequenced after amplification with the polymerase chain reaction, using as primers oligonucleotides corresponding to two conserved sequences. Among 82 unrelated children, 32 HBV DNA variants were present. One sequence was present in 33 children and 31 variants were found among the other 49. Siblings within each of nine families had the same variant; in three families siblings had different variants. Six of the eight fathers and 28 of the 30 mothers had HBV DNA sequences identical to those of their offspring. A total of 34 variants were found among the 134 individuals. The hypothesis of random assortment of sequences in parents and children was rejected (P < .00005). Thus, this newapproach proves the occurrence of intrafamilial transmission of HBV among Chinese.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1093/infdis/164.2.284</identifier><identifier>PMID: 1856476</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Adult ; Base Sequence ; Biological and medical sciences ; Child ; Children ; Disease transmission ; DNA ; DNA, Viral - chemistry ; Family Health ; Female ; Genetic Variation ; Genomes ; Hepatitis antigens ; Hepatitis B - microbiology ; Hepatitis B - transmission ; Hepatitis B Antibodies - blood ; Hepatitis B e Antigens - blood ; Hepatitis B Surface Antigens - blood ; Hepatitis B virus ; Hepatitis B virus - genetics ; Human viral diseases ; Humans ; Infections ; Infectious diseases ; Major Articles ; Male ; Medical sciences ; Molecular Sequence Data ; Mothers ; Nucleotide sequences ; Parents ; Polymorphism, Genetic ; Probability ; Sequence Homology, Nucleic Acid ; Tropical medicine ; Viral diseases ; Viral hepatitis</subject><ispartof>The Journal of infectious diseases, 1991-08, Vol.164 (2), p.284-288</ispartof><rights>Copyright 1991 The University of Chicago</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-f4df76c9b2d4791901ddd57907efa9b73db49b859fe0e2e048a3e0bf6e833fcc3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30111900$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30111900$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>315,782,786,805,27933,27934,58026,58259</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4971267$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1856476$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, Hsiang Ju</creatorcontrib><creatorcontrib>Lai, Ching-lung</creatorcontrib><creatorcontrib>Lauder, I. J.</creatorcontrib><creatorcontrib>Wu, Pui-chee</creatorcontrib><creatorcontrib>Lau, Theodore K.</creatorcontrib><creatorcontrib>Fong, Ming-wei</creatorcontrib><title>Application of Hepatitis B Virus (HBV) DNA Sequence Polymorphisms to the Study of HBV Transmission</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>Short sequencesin hypervariableregions of the hepatitis Bvirus (HBV)genome can beused to identify different strains, providing a novel approach to the study of HBV transmission. The nucleotide sequence in positions 2551–2650 (1: EcoRI site) was determined for serum HBV DNA from 96 Chinese children living in Hong Kong and from 38 of their parents. HBV DNA was extracted and sequenced after amplification with the polymerase chain reaction, using as primers oligonucleotides corresponding to two conserved sequences. Among 82 unrelated children, 32 HBV DNA variants were present. One sequence was present in 33 children and 31 variants were found among the other 49. Siblings within each of nine families had the same variant; in three families siblings had different variants. Six of the eight fathers and 28 of the 30 mothers had HBV DNA sequences identical to those of their offspring. A total of 34 variants were found among the 134 individuals. The hypothesis of random assortment of sequences in parents and children was rejected (P < .00005). Thus, this newapproach proves the occurrence of intrafamilial transmission of HBV among Chinese.</description><subject>Adult</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Children</subject><subject>Disease transmission</subject><subject>DNA</subject><subject>DNA, Viral - chemistry</subject><subject>Family Health</subject><subject>Female</subject><subject>Genetic Variation</subject><subject>Genomes</subject><subject>Hepatitis antigens</subject><subject>Hepatitis B - microbiology</subject><subject>Hepatitis B - transmission</subject><subject>Hepatitis B Antibodies - blood</subject><subject>Hepatitis B e Antigens - blood</subject><subject>Hepatitis B Surface Antigens - blood</subject><subject>Hepatitis B virus</subject><subject>Hepatitis B virus - genetics</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Major Articles</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Mothers</subject><subject>Nucleotide sequences</subject><subject>Parents</subject><subject>Polymorphism, Genetic</subject><subject>Probability</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Tropical medicine</subject><subject>Viral diseases</subject><subject>Viral hepatitis</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkElvEzEAhS0EKqFw54LkA6rgMKm38XJMSmmQIhY1RIiL5fHYqsts2DNS8-9xmdAeOdnSe--T_QHwGqMlRoqeh87XIZ1jzpZkSSR7Aha4pKLgHNOnYIEQIQWWSj0HL1K6RQgxysUJOMGy5EzwBahWw9AEa8bQd7D3cOOGfB9Dgmu4D3FK8N1mvX8PP3xewWv3e3KddfBr3xzaPg43IbUJjj0cbxy8Hqf68Bex3sNdNF1qQ0oZ-xI886ZJ7tXxPAXfP17uLjbF9svVp4vVtrCs5GPhWe0Ft6oiNRMKK4Trui6FQsJ5oypB64qpSpbKO-SIQ0wa6lDluZOUemvpKTibuUPs80PTqPMDrGsa07l-SloigbCU6L9FzAlDlPBcRHPRxj6l6LweYmhNPGiM9L1_PfvPC6aJzv7z5M2RPVWtqx8Hs_Ccvz3mJlnT-OzJZsC_GlMCEy4eMbdp7ONDTBHG2cz9F4o5D2l0dw-5ib90XotSb3781KXaXa3ZVupv9A_Ndac5</recordid><startdate>19910801</startdate><enddate>19910801</enddate><creator>Lin, Hsiang Ju</creator><creator>Lai, Ching-lung</creator><creator>Lauder, I. J.</creator><creator>Wu, Pui-chee</creator><creator>Lau, Theodore K.</creator><creator>Fong, Ming-wei</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19910801</creationdate><title>Application of Hepatitis B Virus (HBV) DNA Sequence Polymorphisms to the Study of HBV Transmission</title><author>Lin, Hsiang Ju ; Lai, Ching-lung ; Lauder, I. J. ; Wu, Pui-chee ; Lau, Theodore K. ; Fong, Ming-wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-f4df76c9b2d4791901ddd57907efa9b73db49b859fe0e2e048a3e0bf6e833fcc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Adult</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Children</topic><topic>Disease transmission</topic><topic>DNA</topic><topic>DNA, Viral - chemistry</topic><topic>Family Health</topic><topic>Female</topic><topic>Genetic Variation</topic><topic>Genomes</topic><topic>Hepatitis antigens</topic><topic>Hepatitis B - microbiology</topic><topic>Hepatitis B - transmission</topic><topic>Hepatitis B Antibodies - blood</topic><topic>Hepatitis B e Antigens - blood</topic><topic>Hepatitis B Surface Antigens - blood</topic><topic>Hepatitis B virus</topic><topic>Hepatitis B virus - genetics</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Major Articles</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>Mothers</topic><topic>Nucleotide sequences</topic><topic>Parents</topic><topic>Polymorphism, Genetic</topic><topic>Probability</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Tropical medicine</topic><topic>Viral diseases</topic><topic>Viral hepatitis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, Hsiang Ju</creatorcontrib><creatorcontrib>Lai, Ching-lung</creatorcontrib><creatorcontrib>Lauder, I. J.</creatorcontrib><creatorcontrib>Wu, Pui-chee</creatorcontrib><creatorcontrib>Lau, Theodore K.</creatorcontrib><creatorcontrib>Fong, Ming-wei</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, Hsiang Ju</au><au>Lai, Ching-lung</au><au>Lauder, I. J.</au><au>Wu, Pui-chee</au><au>Lau, Theodore K.</au><au>Fong, Ming-wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of Hepatitis B Virus (HBV) DNA Sequence Polymorphisms to the Study of HBV Transmission</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>1991-08-01</date><risdate>1991</risdate><volume>164</volume><issue>2</issue><spage>284</spage><epage>288</epage><pages>284-288</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>Short sequencesin hypervariableregions of the hepatitis Bvirus (HBV)genome can beused to identify different strains, providing a novel approach to the study of HBV transmission. The nucleotide sequence in positions 2551–2650 (1: EcoRI site) was determined for serum HBV DNA from 96 Chinese children living in Hong Kong and from 38 of their parents. HBV DNA was extracted and sequenced after amplification with the polymerase chain reaction, using as primers oligonucleotides corresponding to two conserved sequences. Among 82 unrelated children, 32 HBV DNA variants were present. One sequence was present in 33 children and 31 variants were found among the other 49. Siblings within each of nine families had the same variant; in three families siblings had different variants. Six of the eight fathers and 28 of the 30 mothers had HBV DNA sequences identical to those of their offspring. A total of 34 variants were found among the 134 individuals. The hypothesis of random assortment of sequences in parents and children was rejected (P < .00005). Thus, this newapproach proves the occurrence of intrafamilial transmission of HBV among Chinese.</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>1856476</pmid><doi>10.1093/infdis/164.2.284</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of infectious diseases, 1991-08, Vol.164 (2), p.284-288 |
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| subjects | Adult Base Sequence Biological and medical sciences Child Children Disease transmission DNA DNA, Viral - chemistry Family Health Female Genetic Variation Genomes Hepatitis antigens Hepatitis B - microbiology Hepatitis B - transmission Hepatitis B Antibodies - blood Hepatitis B e Antigens - blood Hepatitis B Surface Antigens - blood Hepatitis B virus Hepatitis B virus - genetics Human viral diseases Humans Infections Infectious diseases Major Articles Male Medical sciences Molecular Sequence Data Mothers Nucleotide sequences Parents Polymorphism, Genetic Probability Sequence Homology, Nucleic Acid Tropical medicine Viral diseases Viral hepatitis |
| title | Application of Hepatitis B Virus (HBV) DNA Sequence Polymorphisms to the Study of HBV Transmission |
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