Effect of glibenclamide on extracellular potassium accumulation and the electrophysiological changes during myocardial ischaemia in the arterially perfused interventricular septum of rabbit
Study objective – The aim was to study the effects of glibenclamide on the rate of rise of extracellular potassium concentration ([K+]0) and the electrophysiological changes that occur during myocardial ischaemia. Design – The study was performed in isolated, arterially perfused interventricular sep...
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| Veröffentlicht in: | Cardiovascular research 1991-05, Vol.25 (5), p.407-413 |
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| creator | Hicks, Martin N Cobbe, Stuart M |
| description | Study objective – The aim was to study the effects of glibenclamide on the rate of rise of extracellular potassium concentration ([K+]0) and the electrophysiological changes that occur during myocardial ischaemia. Design – The study was performed in isolated, arterially perfused interventricular septa from the rabbit. Six septa were treated with glibenclamide 10−6 mol·litre−1 and there were six untreated controls (vehicle only). [K+]o and electrophysiological variables were compared before and during a 30 min period of global zero flow ischaemia. Measurements and main results – Prior to ischaemia, the extracellular potassium concentrations measured using potassium sensitive valino-mycin electrodes were similar in the control and glibenclamide groups being 4.0 (SEM 0.1) and 4.0 (0.1) mmol·litre−1 respectively. [K+]0 rose during ischaemia in both groups, and at 30 min was 13.3 (0.7) mmol·litre−1 in the control group. The increase in the glibenclamide group was less marked, reaching 9.2 (0.5) mmol·litre−1 (p |
| doi_str_mv | 10.1093/cvr/25.5.407 |
| format | Article |
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Design – The study was performed in isolated, arterially perfused interventricular septa from the rabbit. Six septa were treated with glibenclamide 10−6 mol·litre−1 and there were six untreated controls (vehicle only). [K+]o and electrophysiological variables were compared before and during a 30 min period of global zero flow ischaemia. Measurements and main results – Prior to ischaemia, the extracellular potassium concentrations measured using potassium sensitive valino-mycin electrodes were similar in the control and glibenclamide groups being 4.0 (SEM 0.1) and 4.0 (0.1) mmol·litre−1 respectively. [K+]0 rose during ischaemia in both groups, and at 30 min was 13.3 (0.7) mmol·litre−1 in the control group. The increase in the glibenclamide group was less marked, reaching 9.2 (0.5) mmol·litre−1 (p<0.0005; unpaired t test). Glibenclamide had no electrophysiological effects prior to ischaemia. However, during ischaemia the decrease in action potential amplitude, action potential duration (APD), maximum upstroke velocity of the action potentials (dV/dtmax), and the extent of resting membrane potential (EM) depolarisation were less in the glibenclamide group than in the controls. The effective refractory period (ERP) progressively shortened over the 30 min of ischaemia in both groups, to a similar extent. When taken in conjunction with the relative changes in action potential duration the degree of post-repolarisation refractoriness (ERP - APD) that developed was less in the glibenclamide group than in the controls. Conclusions – Glibenclamide attenuated the ischaemic rise in [K+]0, with preservation of both membrane potential and action potential amplitude, duration, and upstroke velocity together with less post-repolarisation refractoriness. These effects could be potentially antiarrhythmic in acute myocardial ischaemia.</description><identifier>ISSN: 0008-6363</identifier><identifier>EISSN: 1755-3245</identifier><identifier>DOI: 10.1093/cvr/25.5.407</identifier><identifier>PMID: 1906781</identifier><identifier>CODEN: CVREAU</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Action Potentials - drug effects ; Animals ; Biological and medical sciences ; Coronary Disease - metabolism ; Electrophysiology ; extracellular potassium ; General and cellular metabolism. Vitamins ; glibenclamide ; Glyburide - pharmacology ; Heart Septum - drug effects ; Heart Septum - metabolism ; ischaemia ; Medical sciences ; Membrane Potentials - drug effects ; Organ Culture Techniques ; Perfusion ; Pharmacology. Drug treatments ; Potassium - metabolism ; Rabbits ; Time Factors</subject><ispartof>Cardiovascular research, 1991-05, Vol.25 (5), p.407-413</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3777-cda5d065d11441bb4aff6c28e598b6d03fd7fece594b8e87d5038ee8b36781c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19842940$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1906781$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hicks, Martin N</creatorcontrib><creatorcontrib>Cobbe, Stuart M</creatorcontrib><title>Effect of glibenclamide on extracellular potassium accumulation and the electrophysiological changes during myocardial ischaemia in the arterially perfused interventricular septum of rabbit</title><title>Cardiovascular research</title><addtitle>Cardiovasc Res</addtitle><description>Study objective – The aim was to study the effects of glibenclamide on the rate of rise of extracellular potassium concentration ([K+]0) and the electrophysiological changes that occur during myocardial ischaemia. Design – The study was performed in isolated, arterially perfused interventricular septa from the rabbit. Six septa were treated with glibenclamide 10−6 mol·litre−1 and there were six untreated controls (vehicle only). [K+]o and electrophysiological variables were compared before and during a 30 min period of global zero flow ischaemia. Measurements and main results – Prior to ischaemia, the extracellular potassium concentrations measured using potassium sensitive valino-mycin electrodes were similar in the control and glibenclamide groups being 4.0 (SEM 0.1) and 4.0 (0.1) mmol·litre−1 respectively. [K+]0 rose during ischaemia in both groups, and at 30 min was 13.3 (0.7) mmol·litre−1 in the control group. The increase in the glibenclamide group was less marked, reaching 9.2 (0.5) mmol·litre−1 (p<0.0005; unpaired t test). Glibenclamide had no electrophysiological effects prior to ischaemia. However, during ischaemia the decrease in action potential amplitude, action potential duration (APD), maximum upstroke velocity of the action potentials (dV/dtmax), and the extent of resting membrane potential (EM) depolarisation were less in the glibenclamide group than in the controls. The effective refractory period (ERP) progressively shortened over the 30 min of ischaemia in both groups, to a similar extent. When taken in conjunction with the relative changes in action potential duration the degree of post-repolarisation refractoriness (ERP - APD) that developed was less in the glibenclamide group than in the controls. Conclusions – Glibenclamide attenuated the ischaemic rise in [K+]0, with preservation of both membrane potential and action potential amplitude, duration, and upstroke velocity together with less post-repolarisation refractoriness. These effects could be potentially antiarrhythmic in acute myocardial ischaemia.</description><subject>Action Potentials - drug effects</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Coronary Disease - metabolism</subject><subject>Electrophysiology</subject><subject>extracellular potassium</subject><subject>General and cellular metabolism. Vitamins</subject><subject>glibenclamide</subject><subject>Glyburide - pharmacology</subject><subject>Heart Septum - drug effects</subject><subject>Heart Septum - metabolism</subject><subject>ischaemia</subject><subject>Medical sciences</subject><subject>Membrane Potentials - drug effects</subject><subject>Organ Culture Techniques</subject><subject>Perfusion</subject><subject>Pharmacology. Drug treatments</subject><subject>Potassium - metabolism</subject><subject>Rabbits</subject><subject>Time Factors</subject><issn>0008-6363</issn><issn>1755-3245</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkU9v1DAQxSMEKkvhxhXJFziRrRPHsXNEVUupVkJIFap6sRx7vGtw_mA7VffD8d2YdlfAyZp5P4-e3iuKtxVdV7RjZ-Y-ntV8zdcNFc-KVSU4L1nd8OfFilIqy5a17GXxKqUfOHIumpPipOpoK2S1Kn5fOAcmk8mRbfA9jCbowVsg00jgIUdtIIQl6EjmKeuU_DIQbcwy4C57hPRoSd4BgYBn4jTv9slPYdp6owMxOz1uIRG7RD9uybCfjI7Wo-ITajB4Tfz49F_HDBGVsCczRLcksCjh7h7GHL158pBgzmgAzUbd9z6_Ll44HRK8Ob6nxc3lxc35Vbn5-vnL-adNaZgQojRWc0tbbquqaaq-b7Rzrakl8E72raXMWYEp4Nj0EqSwnDIJIHv2GJJhp8WHw9k5Tr8WSFkN6B-D0SNMS1KStl3XSongxwNo4pRSBKfm6Acd96qi6rEshWWpmiuusCzE3x3vLv0A9h98aAf190ddJ4zTRT0an_7DZFN3DUWuPHA-ZXj4q-v4U7WCCa6ubu_U7ffL6zt-vVHf2B9Q9LPT</recordid><startdate>199105</startdate><enddate>199105</enddate><creator>Hicks, Martin N</creator><creator>Cobbe, Stuart M</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199105</creationdate><title>Effect of glibenclamide on extracellular potassium accumulation and the electrophysiological changes during myocardial ischaemia in the arterially perfused interventricular septum of rabbit</title><author>Hicks, Martin N ; Cobbe, Stuart M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3777-cda5d065d11441bb4aff6c28e598b6d03fd7fece594b8e87d5038ee8b36781c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Action Potentials - drug effects</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Coronary Disease - metabolism</topic><topic>Electrophysiology</topic><topic>extracellular potassium</topic><topic>General and cellular metabolism. Vitamins</topic><topic>glibenclamide</topic><topic>Glyburide - pharmacology</topic><topic>Heart Septum - drug effects</topic><topic>Heart Septum - metabolism</topic><topic>ischaemia</topic><topic>Medical sciences</topic><topic>Membrane Potentials - drug effects</topic><topic>Organ Culture Techniques</topic><topic>Perfusion</topic><topic>Pharmacology. Drug treatments</topic><topic>Potassium - metabolism</topic><topic>Rabbits</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hicks, Martin N</creatorcontrib><creatorcontrib>Cobbe, Stuart M</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cardiovascular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hicks, Martin N</au><au>Cobbe, Stuart M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of glibenclamide on extracellular potassium accumulation and the electrophysiological changes during myocardial ischaemia in the arterially perfused interventricular septum of rabbit</atitle><jtitle>Cardiovascular research</jtitle><addtitle>Cardiovasc Res</addtitle><date>1991-05</date><risdate>1991</risdate><volume>25</volume><issue>5</issue><spage>407</spage><epage>413</epage><pages>407-413</pages><issn>0008-6363</issn><eissn>1755-3245</eissn><coden>CVREAU</coden><abstract>Study objective – The aim was to study the effects of glibenclamide on the rate of rise of extracellular potassium concentration ([K+]0) and the electrophysiological changes that occur during myocardial ischaemia. Design – The study was performed in isolated, arterially perfused interventricular septa from the rabbit. Six septa were treated with glibenclamide 10−6 mol·litre−1 and there were six untreated controls (vehicle only). [K+]o and electrophysiological variables were compared before and during a 30 min period of global zero flow ischaemia. Measurements and main results – Prior to ischaemia, the extracellular potassium concentrations measured using potassium sensitive valino-mycin electrodes were similar in the control and glibenclamide groups being 4.0 (SEM 0.1) and 4.0 (0.1) mmol·litre−1 respectively. [K+]0 rose during ischaemia in both groups, and at 30 min was 13.3 (0.7) mmol·litre−1 in the control group. The increase in the glibenclamide group was less marked, reaching 9.2 (0.5) mmol·litre−1 (p<0.0005; unpaired t test). Glibenclamide had no electrophysiological effects prior to ischaemia. However, during ischaemia the decrease in action potential amplitude, action potential duration (APD), maximum upstroke velocity of the action potentials (dV/dtmax), and the extent of resting membrane potential (EM) depolarisation were less in the glibenclamide group than in the controls. The effective refractory period (ERP) progressively shortened over the 30 min of ischaemia in both groups, to a similar extent. When taken in conjunction with the relative changes in action potential duration the degree of post-repolarisation refractoriness (ERP - APD) that developed was less in the glibenclamide group than in the controls. Conclusions – Glibenclamide attenuated the ischaemic rise in [K+]0, with preservation of both membrane potential and action potential amplitude, duration, and upstroke velocity together with less post-repolarisation refractoriness. These effects could be potentially antiarrhythmic in acute myocardial ischaemia.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>1906781</pmid><doi>10.1093/cvr/25.5.407</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Cardiovascular research, 1991-05, Vol.25 (5), p.407-413 |
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| subjects | Action Potentials - drug effects Animals Biological and medical sciences Coronary Disease - metabolism Electrophysiology extracellular potassium General and cellular metabolism. Vitamins glibenclamide Glyburide - pharmacology Heart Septum - drug effects Heart Septum - metabolism ischaemia Medical sciences Membrane Potentials - drug effects Organ Culture Techniques Perfusion Pharmacology. Drug treatments Potassium - metabolism Rabbits Time Factors |
| title | Effect of glibenclamide on extracellular potassium accumulation and the electrophysiological changes during myocardial ischaemia in the arterially perfused interventricular septum of rabbit |
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