DNA discontinuities in the domain of amplified human MYC oncogenes
COLO320DM and COLO320HSR are cell lines derived from a human colon carcinoma, Both lines have an amplification of the MYC oncogene: COLO320DM in the form of double minute chromosomes, COLO320HSR as a large marker chromosome with homogeneously staining regions. We have used pulsed field gel electroph...
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| Veröffentlicht in: | Genes chromosomes & cancer 1991-03, Vol.3 (2), p.136-141 |
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| creator | Bianchi, Néstor O. Bianchi, Martha S. Kere, Juha |
| description | COLO320DM and COLO320HSR are cell lines derived from a human colon carcinoma, Both lines have an amplification of the MYC oncogene: COLO320DM in the form of double minute chromosomes, COLO320HSR as a large marker chromosome with homogeneously staining regions. We have used pulsed field gel electrophoresis (PFGE) to analyze undigested DNA from both COLO320 cell lines. Upon blotting and hybridization with a MYC probe, the autoradiograms showed the existence of discontinuities in the amplified DNA domains. Additional evidence indicating a preferential concentration of DNA breaks in the region containing the MYC amplicons was obtained with the alkaline unwinding technique. We propose that amplicons are connected by hairpin formation or Hoogsteen pairing between free DNA ends. |
| doi_str_mv | 10.1002/gcc.2870030209 |
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We have used pulsed field gel electrophoresis (PFGE) to analyze undigested DNA from both COLO320 cell lines. Upon blotting and hybridization with a MYC probe, the autoradiograms showed the existence of discontinuities in the amplified DNA domains. Additional evidence indicating a preferential concentration of DNA breaks in the region containing the MYC amplicons was obtained with the alkaline unwinding technique. We propose that amplicons are connected by hairpin formation or Hoogsteen pairing between free DNA ends.</description><identifier>ISSN: 1045-2257</identifier><identifier>EISSN: 1098-2264</identifier><identifier>DOI: 10.1002/gcc.2870030209</identifier><identifier>PMID: 2069911</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adenocarcinoma - genetics ; Adenocarcinoma - pathology ; Colonic Neoplasms - genetics ; Colonic Neoplasms - pathology ; DNA, Neoplasm - genetics ; Electrophoresis, Agar Gel ; Gene Amplification ; Genes, myc ; Genetic Markers ; Humans ; Tumor Cells, Cultured - ultrastructure</subject><ispartof>Genes chromosomes & cancer, 1991-03, Vol.3 (2), p.136-141</ispartof><rights>Copyright © 1991 Wiley‐Liss, Inc., A Wiley Company</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3789-114c94f732451d205a0197462ae7a69d9fb7bd922ba796efc708e8bfd33547cc3</citedby><cites>FETCH-LOGICAL-c3789-114c94f732451d205a0197462ae7a69d9fb7bd922ba796efc708e8bfd33547cc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fgcc.2870030209$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fgcc.2870030209$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2069911$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bianchi, Néstor O.</creatorcontrib><creatorcontrib>Bianchi, Martha S.</creatorcontrib><creatorcontrib>Kere, Juha</creatorcontrib><title>DNA discontinuities in the domain of amplified human MYC oncogenes</title><title>Genes chromosomes & cancer</title><addtitle>Genes Chromosom. Cancer</addtitle><description>COLO320DM and COLO320HSR are cell lines derived from a human colon carcinoma, Both lines have an amplification of the MYC oncogene: COLO320DM in the form of double minute chromosomes, COLO320HSR as a large marker chromosome with homogeneously staining regions. We have used pulsed field gel electrophoresis (PFGE) to analyze undigested DNA from both COLO320 cell lines. Upon blotting and hybridization with a MYC probe, the autoradiograms showed the existence of discontinuities in the amplified DNA domains. Additional evidence indicating a preferential concentration of DNA breaks in the region containing the MYC amplicons was obtained with the alkaline unwinding technique. We propose that amplicons are connected by hairpin formation or Hoogsteen pairing between free DNA ends.</description><subject>Adenocarcinoma - genetics</subject><subject>Adenocarcinoma - pathology</subject><subject>Colonic Neoplasms - genetics</subject><subject>Colonic Neoplasms - pathology</subject><subject>DNA, Neoplasm - genetics</subject><subject>Electrophoresis, Agar Gel</subject><subject>Gene Amplification</subject><subject>Genes, myc</subject><subject>Genetic Markers</subject><subject>Humans</subject><subject>Tumor Cells, Cultured - ultrastructure</subject><issn>1045-2257</issn><issn>1098-2264</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDFPwzAUhC0EKqWwsiFlYkt5tpM4HkuAgtSWBYRgsRzHaQ1JXOJE0H9PqlRFTEzvpLv79HQInWMYYwBytVRqTGIGQIEAP0BDDDz2CYmCw60Owk6H7BidOPcOABHl4QANCEScYzxE1zeLiZcZp2zVmKo1jdHOM5XXrLSX2VJ20uaeLNeFyY3OvFVbysqbvyaerZRd6kq7U3SUy8Lps90doee726fk3p89Th-SycxXlMXcxzhQPMgZJUGIMwKhBMxZEBGpmYx4xvOUpRknJJWMRzpXDGIdp3lGaRgwpegIXfbcdW0_W-0aUXZ_66KQlbatEzFEMYRAuuC4D6raOlfrXKxrU8p6IzCI7WiiG038jtYVLnbkNi11to_vVup83vtfptCbf2himiR_2H7fNa7R3_uurD9ExCgLxctiKhLGkzc-TwTQH-XGhak</recordid><startdate>199103</startdate><enddate>199103</enddate><creator>Bianchi, Néstor O.</creator><creator>Bianchi, Martha S.</creator><creator>Kere, Juha</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199103</creationdate><title>DNA discontinuities in the domain of amplified human MYC oncogenes</title><author>Bianchi, Néstor O. ; Bianchi, Martha S. ; Kere, Juha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3789-114c94f732451d205a0197462ae7a69d9fb7bd922ba796efc708e8bfd33547cc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Adenocarcinoma - genetics</topic><topic>Adenocarcinoma - pathology</topic><topic>Colonic Neoplasms - genetics</topic><topic>Colonic Neoplasms - pathology</topic><topic>DNA, Neoplasm - genetics</topic><topic>Electrophoresis, Agar Gel</topic><topic>Gene Amplification</topic><topic>Genes, myc</topic><topic>Genetic Markers</topic><topic>Humans</topic><topic>Tumor Cells, Cultured - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bianchi, Néstor O.</creatorcontrib><creatorcontrib>Bianchi, Martha S.</creatorcontrib><creatorcontrib>Kere, Juha</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Genes chromosomes & cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bianchi, Néstor O.</au><au>Bianchi, Martha S.</au><au>Kere, Juha</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA discontinuities in the domain of amplified human MYC oncogenes</atitle><jtitle>Genes chromosomes & cancer</jtitle><addtitle>Genes Chromosom. Cancer</addtitle><date>1991-03</date><risdate>1991</risdate><volume>3</volume><issue>2</issue><spage>136</spage><epage>141</epage><pages>136-141</pages><issn>1045-2257</issn><eissn>1098-2264</eissn><abstract>COLO320DM and COLO320HSR are cell lines derived from a human colon carcinoma, Both lines have an amplification of the MYC oncogene: COLO320DM in the form of double minute chromosomes, COLO320HSR as a large marker chromosome with homogeneously staining regions. We have used pulsed field gel electrophoresis (PFGE) to analyze undigested DNA from both COLO320 cell lines. Upon blotting and hybridization with a MYC probe, the autoradiograms showed the existence of discontinuities in the amplified DNA domains. Additional evidence indicating a preferential concentration of DNA breaks in the region containing the MYC amplicons was obtained with the alkaline unwinding technique. 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| ispartof | Genes chromosomes & cancer, 1991-03, Vol.3 (2), p.136-141 |
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| subjects | Adenocarcinoma - genetics Adenocarcinoma - pathology Colonic Neoplasms - genetics Colonic Neoplasms - pathology DNA, Neoplasm - genetics Electrophoresis, Agar Gel Gene Amplification Genes, myc Genetic Markers Humans Tumor Cells, Cultured - ultrastructure |
| title | DNA discontinuities in the domain of amplified human MYC oncogenes |
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