Purification of human tumor cell autocrine motility factor and molecular cloning of its receptor

Tumor autocrine motility factor (AMF) has been detected in and purified from serum-free conditioned medium of human HT-1080 fibrosarcoma cells. Under nonreducing conditions, AMF migrates in sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a single band of 55 kDa but under reducing condit...

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Veröffentlicht in:	The Journal of biological chemistry 1991-07, Vol.266 (20), p.13442-13448
Hauptverfasser:	WATANABE, H, CARMI, P, HOGAN, V, RAZ, T, SILLETTI, S, NABI, I. R, RAZ, A
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analysis of the immune response. Humoral and cellular immunity Antibodies, Monoclonal Base Sequence Biological and medical sciences Biomarkers, Tumor - isolation & purification Cell Line Cell Movement - drug effects Cloning, Molecular DNA, Neoplasm - genetics DNA, Neoplasm - isolation & purification Fibrosarcoma Fundamental and applied biological sciences. Psychology Fundamental immunology Gene Library genes Glucose-6-Phosphate Isomerase HeLa Cells - physiology Humans Immunobiology Miscellaneous Molecular Sequence Data Neoplasm Proteins - isolation & purification Neoplasm Proteins - metabolism Neoplasm Proteins - pharmacology p53 protein Receptors, Autocrine Motility Factor Receptors, Cell Surface - genetics Receptors, Cytokine Regulatory factors and their cellular receptors RNA, Neoplasm - genetics RNA, Neoplasm - isolation & purification Sequence Homology, Nucleic Acid Tumor Suppressor Protein p53 - genetics Ubiquitin-Protein Ligases
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