Long-term culture of murine erythropoietic progenitor cells in the absence of an adherent layer
Replication of multipotential stem cells in long-term murine bone marrow cell culture is known to depend on the development of an adherent stromal cell layer. In these conditions, restricted haematopoietic progenitor cells have also been generated for up to several months 1–3 . However, maturation i...
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| Veröffentlicht in: | Nature (London) 1983-10, Vol.305 (5935), p.625-627 |
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| creator | Wendling, Françoise Shreeve, Mona M McLeod, David L Axelrad, Arthur A |
| description | Replication of multipotential stem cells in long-term murine bone marrow cell culture is known to depend on the development of an adherent stromal cell layer. In these conditions, restricted haematopoietic progenitor cells have also been generated for up to several months
1–3
. However, maturation is observed only in the granulocyte/macrophage and megakaryocyte lineages; erythropoiesis appears to be blocked at the earliest burst-forming unit (BFU-E) stage. Addition of exogenous erythropoietin (Epo) or anaemic mouse serum results in full erythropoietic maturation, but it is transient
4,5
. We describe here a culture system in which production of erythropoietic progenitor cells can be maintained for over 6 months in the absence of an adherent stromal layer and in the absence of added Epo, but in the presence of pokeweed mitogen-stimulated spleen cell conditioned medium (PWSCM). The data indicate that restricted erythroid progenitor cells exist which are capable of extensive self-renewal. |
| doi_str_mv | 10.1038/305625a0 |
| format | Article |
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1–3
. However, maturation is observed only in the granulocyte/macrophage and megakaryocyte lineages; erythropoiesis appears to be blocked at the earliest burst-forming unit (BFU-E) stage. Addition of exogenous erythropoietin (Epo) or anaemic mouse serum results in full erythropoietic maturation, but it is transient
4,5
. We describe here a culture system in which production of erythropoietic progenitor cells can be maintained for over 6 months in the absence of an adherent stromal layer and in the absence of added Epo, but in the presence of pokeweed mitogen-stimulated spleen cell conditioned medium (PWSCM). The data indicate that restricted erythroid progenitor cells exist which are capable of extensive self-renewal.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/305625a0</identifier><identifier>PMID: 6621710</identifier><identifier>CODEN: NATUAS</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Animal cells ; Animals ; Biological and medical sciences ; Biotechnology ; Bone Marrow - physiology ; Cell Adhesion ; Cell cultures. Hybridization. Fusion ; Cell Division ; Cells, Cultured ; Culture Media ; Erythropoiesis ; Establishment of new cell lines, improvement of cultural methods, mass cultures ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; Hematopoietic Stem Cells - physiology ; Humanities and Social Sciences ; Kinetics ; letter ; Methods. Procedures. Technologies ; Mice ; Mice, Inbred C57BL ; Molecular and cellular biology ; multidisciplinary ; Science ; Science (multidisciplinary) ; Time Factors</subject><ispartof>Nature (London), 1983-10, Vol.305 (5935), p.625-627</ispartof><rights>Springer Nature Limited 1983</rights><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c364t-e9ac8e5d07f7d95ba48a08f6fa4c857135c02f182ca1a45bf2f64fb508f1c5b53</citedby><cites>FETCH-LOGICAL-c364t-e9ac8e5d07f7d95ba48a08f6fa4c857135c02f182ca1a45bf2f64fb508f1c5b53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2727,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9344692$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6621710$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wendling, Françoise</creatorcontrib><creatorcontrib>Shreeve, Mona M</creatorcontrib><creatorcontrib>McLeod, David L</creatorcontrib><creatorcontrib>Axelrad, Arthur A</creatorcontrib><title>Long-term culture of murine erythropoietic progenitor cells in the absence of an adherent layer</title><title>Nature (London)</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>Replication of multipotential stem cells in long-term murine bone marrow cell culture is known to depend on the development of an adherent stromal cell layer. In these conditions, restricted haematopoietic progenitor cells have also been generated for up to several months
1–3
. However, maturation is observed only in the granulocyte/macrophage and megakaryocyte lineages; erythropoiesis appears to be blocked at the earliest burst-forming unit (BFU-E) stage. Addition of exogenous erythropoietin (Epo) or anaemic mouse serum results in full erythropoietic maturation, but it is transient
4,5
. We describe here a culture system in which production of erythropoietic progenitor cells can be maintained for over 6 months in the absence of an adherent stromal layer and in the absence of added Epo, but in the presence of pokeweed mitogen-stimulated spleen cell conditioned medium (PWSCM). The data indicate that restricted erythroid progenitor cells exist which are capable of extensive self-renewal.</description><subject>Animal cells</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Bone Marrow - physiology</subject><subject>Cell Adhesion</subject><subject>Cell cultures. Hybridization. Fusion</subject><subject>Cell Division</subject><subject>Cells, Cultured</subject><subject>Culture Media</subject><subject>Erythropoiesis</subject><subject>Establishment of new cell lines, improvement of cultural methods, mass cultures</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hematopoietic Stem Cells - physiology</subject><subject>Humanities and Social Sciences</subject><subject>Kinetics</subject><subject>letter</subject><subject>Methods. Procedures. Technologies</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Molecular and cellular biology</subject><subject>multidisciplinary</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Time Factors</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkU9P4zAQxS3EqpQ_El8A5ANC7CFgJ7bjHhFaYKVKe1nO0cQdt6kSu9jOod9-3W3pidMc3m_ezLwh5JqzR84q_VQxqUoJ7IRMuahVIZSuT8mUsVIXTFfqjJzHuGaMSV6LCZkoVfKasylp5t4ti4RhoGbs0xiQekuHMXQOKYZtWgW_8R2mztBN8Et0XfKBGuz7SDtH0woptBGd-d8IjsJihQFdoj1sMVySHxb6iFeHekE-Xn_9fXkv5n_efr88zwtTKZEKnIHRKBestvViJlsQGpi2yoIwWta8koaVluvSAAchW1taJWwrM8ONbGV1Qe73vnnJzxFjaoYu7rYEh36MjWaqzgfrDD7sQRN8jAFtswndAGHbcNbssmy-sszozcFzbAdcHMFDeFm_O-gQDfQ2gDNdPGKzSgg1KzP2c4_FrLglhmbtx-ByGt-NvN2zDna_OHodgX8wQ5Ni</recordid><startdate>19831013</startdate><enddate>19831013</enddate><creator>Wendling, Françoise</creator><creator>Shreeve, Mona M</creator><creator>McLeod, David L</creator><creator>Axelrad, Arthur A</creator><general>Nature Publishing Group UK</general><general>Nature Publishing</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19831013</creationdate><title>Long-term culture of murine erythropoietic progenitor cells in the absence of an adherent layer</title><author>Wendling, Françoise ; Shreeve, Mona M ; McLeod, David L ; Axelrad, Arthur A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c364t-e9ac8e5d07f7d95ba48a08f6fa4c857135c02f182ca1a45bf2f64fb508f1c5b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Animal cells</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Bone Marrow - physiology</topic><topic>Cell Adhesion</topic><topic>Cell cultures. Hybridization. Fusion</topic><topic>Cell Division</topic><topic>Cells, Cultured</topic><topic>Culture Media</topic><topic>Erythropoiesis</topic><topic>Establishment of new cell lines, improvement of cultural methods, mass cultures</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hematopoietic Stem Cells - physiology</topic><topic>Humanities and Social Sciences</topic><topic>Kinetics</topic><topic>letter</topic><topic>Methods. Procedures. Technologies</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Molecular and cellular biology</topic><topic>multidisciplinary</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wendling, Françoise</creatorcontrib><creatorcontrib>Shreeve, Mona M</creatorcontrib><creatorcontrib>McLeod, David L</creatorcontrib><creatorcontrib>Axelrad, Arthur A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nature (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wendling, Françoise</au><au>Shreeve, Mona M</au><au>McLeod, David L</au><au>Axelrad, Arthur A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Long-term culture of murine erythropoietic progenitor cells in the absence of an adherent layer</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>1983-10-13</date><risdate>1983</risdate><volume>305</volume><issue>5935</issue><spage>625</spage><epage>627</epage><pages>625-627</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><coden>NATUAS</coden><abstract>Replication of multipotential stem cells in long-term murine bone marrow cell culture is known to depend on the development of an adherent stromal cell layer. In these conditions, restricted haematopoietic progenitor cells have also been generated for up to several months
1–3
. However, maturation is observed only in the granulocyte/macrophage and megakaryocyte lineages; erythropoiesis appears to be blocked at the earliest burst-forming unit (BFU-E) stage. Addition of exogenous erythropoietin (Epo) or anaemic mouse serum results in full erythropoietic maturation, but it is transient
4,5
. We describe here a culture system in which production of erythropoietic progenitor cells can be maintained for over 6 months in the absence of an adherent stromal layer and in the absence of added Epo, but in the presence of pokeweed mitogen-stimulated spleen cell conditioned medium (PWSCM). The data indicate that restricted erythroid progenitor cells exist which are capable of extensive self-renewal.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>6621710</pmid><doi>10.1038/305625a0</doi><tpages>3</tpages></addata></record> |
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| ispartof | Nature (London), 1983-10, Vol.305 (5935), p.625-627 |
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| subjects | Animal cells Animals Biological and medical sciences Biotechnology Bone Marrow - physiology Cell Adhesion Cell cultures. Hybridization. Fusion Cell Division Cells, Cultured Culture Media Erythropoiesis Establishment of new cell lines, improvement of cultural methods, mass cultures Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Hematopoietic Stem Cells - physiology Humanities and Social Sciences Kinetics letter Methods. Procedures. Technologies Mice Mice, Inbred C57BL Molecular and cellular biology multidisciplinary Science Science (multidisciplinary) Time Factors |
| title | Long-term culture of murine erythropoietic progenitor cells in the absence of an adherent layer |
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