Induction of Cholinergic Expression in Developing Spinal Cord Cultures
: The induction of choline acetyltransferase (ChAT) by cAMP derivatives was studied in dissociated spinal cord cultures. Dibutyryl cAMP (dbcAMP) and 8‐bromo cAMP (1 mM) produced a 2–3‐fold stimulation of ChAT activity in developing cultures whereas 8‐bromo cGMP had no effect. A phosphodiesterase inh...
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| Veröffentlicht in: | Journal of neurochemistry 1983-01, Vol.41 (5), p.1349-1356 |
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| description | : The induction of choline acetyltransferase (ChAT) by cAMP derivatives was studied in dissociated spinal cord cultures. Dibutyryl cAMP (dbcAMP) and 8‐bromo cAMP (1 mM) produced a 2–3‐fold stimulation of ChAT activity in developing cultures whereas 8‐bromo cGMP had no effect. A phosphodiesterase inhibitor, 3‐isobutyl‐1‐methylxanthine, also increased (2‐fold) ChAT activity in immature cultures. Significant elevations in ChAT were detected after 2 h incubation with dbcAMP. Maximum enzyme induction was observed 24 h after dbcAMP supplementation to the culture medium. Developmental studies revealed that ChAT could be induced on days 2–16 in culture. The largest induction of ChAT activity was observed on day 7 in culture. After day 19, when control enzyme activity attained levels of mature cultures, cAMP‐mediated ChAT induction was no longer observed. Cycloheximide and actinomycin D blocked ChAT induction whereas basal enzyme activity remained unaffected. Culture protein content was not changed after 1‐day exposure to dbcAMP. 125I‐Tetanus toxin fixation after dbcAMP treatment revealed a 20% decrease from control in neuronal surface during days 7–9 in culture. These data indicated that cAMP derivatives produced a rapid increase in cholinergic expression during a specific period of development in spinal cord cultures. There appears to be specificity to this effect, as total neuronal surface does not respond in the same manner as ChAT activity. The ChAT induction mediated by dbcAMP appears to be regulated at the transcriptional level. |
| doi_str_mv | 10.1111/j.1471-4159.1983.tb00832.x |
| format | Article |
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Dibutyryl cAMP (dbcAMP) and 8‐bromo cAMP (1 mM) produced a 2–3‐fold stimulation of ChAT activity in developing cultures whereas 8‐bromo cGMP had no effect. A phosphodiesterase inhibitor, 3‐isobutyl‐1‐methylxanthine, also increased (2‐fold) ChAT activity in immature cultures. Significant elevations in ChAT were detected after 2 h incubation with dbcAMP. Maximum enzyme induction was observed 24 h after dbcAMP supplementation to the culture medium. Developmental studies revealed that ChAT could be induced on days 2–16 in culture. The largest induction of ChAT activity was observed on day 7 in culture. After day 19, when control enzyme activity attained levels of mature cultures, cAMP‐mediated ChAT induction was no longer observed. Cycloheximide and actinomycin D blocked ChAT induction whereas basal enzyme activity remained unaffected. Culture protein content was not changed after 1‐day exposure to dbcAMP. 125I‐Tetanus toxin fixation after dbcAMP treatment revealed a 20% decrease from control in neuronal surface during days 7–9 in culture. These data indicated that cAMP derivatives produced a rapid increase in cholinergic expression during a specific period of development in spinal cord cultures. There appears to be specificity to this effect, as total neuronal surface does not respond in the same manner as ChAT activity. The ChAT induction mediated by dbcAMP appears to be regulated at the transcriptional level.</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1111/j.1471-4159.1983.tb00832.x</identifier><identifier>PMID: 6194265</identifier><identifier>CODEN: JONRA9</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>1-Methyl-3-isobutylxanthine - pharmacology ; 8-Bromo Cyclic Adenosine Monophosphate - pharmacology ; Animals ; Biological and medical sciences ; Bucladesine - pharmacology ; Cell physiology ; Choline O-Acetyltransferase - biosynthesis ; Cholinergic ; Cyclic AMP ; Cycloheximide - pharmacology ; Dactinomycin - pharmacology ; Development ; Enzyme Induction ; Female ; Fundamental and applied biological sciences. Psychology ; Induction ; Mice ; Molecular and cellular biology ; Neuron ; Neurotransmission ; Pregnancy ; Spinal cord ; Spinal Cord - drug effects ; Spinal Cord - enzymology ; Time Factors</subject><ispartof>Journal of neurochemistry, 1983-01, Vol.41 (5), p.1349-1356</ispartof><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4309-954d9f9b054a006494d3dbabe7291d56b21bee4997e4a0283b117d2d4ae105d03</citedby><cites>FETCH-LOGICAL-c4309-954d9f9b054a006494d3dbabe7291d56b21bee4997e4a0283b117d2d4ae105d03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1471-4159.1983.tb00832.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1471-4159.1983.tb00832.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27911,27912,45561,45562</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9397104$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6194265$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brenneman, Douglas E.</creatorcontrib><creatorcontrib>Warren, Dale</creatorcontrib><title>Induction of Cholinergic Expression in Developing Spinal Cord Cultures</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>: The induction of choline acetyltransferase (ChAT) by cAMP derivatives was studied in dissociated spinal cord cultures. Dibutyryl cAMP (dbcAMP) and 8‐bromo cAMP (1 mM) produced a 2–3‐fold stimulation of ChAT activity in developing cultures whereas 8‐bromo cGMP had no effect. A phosphodiesterase inhibitor, 3‐isobutyl‐1‐methylxanthine, also increased (2‐fold) ChAT activity in immature cultures. Significant elevations in ChAT were detected after 2 h incubation with dbcAMP. Maximum enzyme induction was observed 24 h after dbcAMP supplementation to the culture medium. Developmental studies revealed that ChAT could be induced on days 2–16 in culture. The largest induction of ChAT activity was observed on day 7 in culture. After day 19, when control enzyme activity attained levels of mature cultures, cAMP‐mediated ChAT induction was no longer observed. Cycloheximide and actinomycin D blocked ChAT induction whereas basal enzyme activity remained unaffected. Culture protein content was not changed after 1‐day exposure to dbcAMP. 125I‐Tetanus toxin fixation after dbcAMP treatment revealed a 20% decrease from control in neuronal surface during days 7–9 in culture. These data indicated that cAMP derivatives produced a rapid increase in cholinergic expression during a specific period of development in spinal cord cultures. There appears to be specificity to this effect, as total neuronal surface does not respond in the same manner as ChAT activity. The ChAT induction mediated by dbcAMP appears to be regulated at the transcriptional level.</description><subject>1-Methyl-3-isobutylxanthine - pharmacology</subject><subject>8-Bromo Cyclic Adenosine Monophosphate - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bucladesine - pharmacology</subject><subject>Cell physiology</subject><subject>Choline O-Acetyltransferase - biosynthesis</subject><subject>Cholinergic</subject><subject>Cyclic AMP</subject><subject>Cycloheximide - pharmacology</subject><subject>Dactinomycin - pharmacology</subject><subject>Development</subject><subject>Enzyme Induction</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Induction</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Neuron</subject><subject>Neurotransmission</subject><subject>Pregnancy</subject><subject>Spinal cord</subject><subject>Spinal Cord - drug effects</subject><subject>Spinal Cord - enzymology</subject><subject>Time Factors</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkE1P3DAQhi1EBVvKT0CKKtRb0hnb-XAvCKXLR4XaQ8vZcmIHvPImi72hy7_H0UZ7rerD-PA-M2M_hHxGyDCer6sMeYkpx1xkKCqWbRuAitFsd0QWh-iYLAAoTRlweko-hrACwIIXeEJOChScFvmC3Nz3emy3duiToUvq58HZ3vgn2ybL3cabEKbE9sl382rcsLH9U_I7VuWSevA6qUe3HSP2iXzolAvmfL7PyOPN8k99lz78ur2vrx_SljMQqci5Fp1oIOcKoOCCa6Yb1ZiSCtR50VBsjOFClCYCtGINYqmp5sog5BrYGfmyn7vxw8towlaubWiNc6o3wxhkBUUZv5b_E0RWRWVlFcFve7D1QwjedHLj7Vr5N4kgJ9tyJSelclIqJ9tyti13sfli3jI2a6MPrbPemF_OuQqtcp1XfWvDARNMlAg8Yld77K915u0_HiB__KyRccHeAXg_mz8</recordid><startdate>19830101</startdate><enddate>19830101</enddate><creator>Brenneman, Douglas E.</creator><creator>Warren, Dale</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19830101</creationdate><title>Induction of Cholinergic Expression in Developing Spinal Cord Cultures</title><author>Brenneman, Douglas E. ; Warren, Dale</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4309-954d9f9b054a006494d3dbabe7291d56b21bee4997e4a0283b117d2d4ae105d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>1-Methyl-3-isobutylxanthine - pharmacology</topic><topic>8-Bromo Cyclic Adenosine Monophosphate - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bucladesine - pharmacology</topic><topic>Cell physiology</topic><topic>Choline O-Acetyltransferase - biosynthesis</topic><topic>Cholinergic</topic><topic>Cyclic AMP</topic><topic>Cycloheximide - pharmacology</topic><topic>Dactinomycin - pharmacology</topic><topic>Development</topic><topic>Enzyme Induction</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Induction</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Neuron</topic><topic>Neurotransmission</topic><topic>Pregnancy</topic><topic>Spinal cord</topic><topic>Spinal Cord - drug effects</topic><topic>Spinal Cord - enzymology</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brenneman, Douglas E.</creatorcontrib><creatorcontrib>Warren, Dale</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brenneman, Douglas E.</au><au>Warren, Dale</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of Cholinergic Expression in Developing Spinal Cord Cultures</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>1983-01-01</date><risdate>1983</risdate><volume>41</volume><issue>5</issue><spage>1349</spage><epage>1356</epage><pages>1349-1356</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><coden>JONRA9</coden><abstract>: The induction of choline acetyltransferase (ChAT) by cAMP derivatives was studied in dissociated spinal cord cultures. Dibutyryl cAMP (dbcAMP) and 8‐bromo cAMP (1 mM) produced a 2–3‐fold stimulation of ChAT activity in developing cultures whereas 8‐bromo cGMP had no effect. A phosphodiesterase inhibitor, 3‐isobutyl‐1‐methylxanthine, also increased (2‐fold) ChAT activity in immature cultures. Significant elevations in ChAT were detected after 2 h incubation with dbcAMP. Maximum enzyme induction was observed 24 h after dbcAMP supplementation to the culture medium. Developmental studies revealed that ChAT could be induced on days 2–16 in culture. The largest induction of ChAT activity was observed on day 7 in culture. After day 19, when control enzyme activity attained levels of mature cultures, cAMP‐mediated ChAT induction was no longer observed. Cycloheximide and actinomycin D blocked ChAT induction whereas basal enzyme activity remained unaffected. Culture protein content was not changed after 1‐day exposure to dbcAMP. 125I‐Tetanus toxin fixation after dbcAMP treatment revealed a 20% decrease from control in neuronal surface during days 7–9 in culture. These data indicated that cAMP derivatives produced a rapid increase in cholinergic expression during a specific period of development in spinal cord cultures. There appears to be specificity to this effect, as total neuronal surface does not respond in the same manner as ChAT activity. The ChAT induction mediated by dbcAMP appears to be regulated at the transcriptional level.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>6194265</pmid><doi>10.1111/j.1471-4159.1983.tb00832.x</doi><tpages>8</tpages></addata></record> |
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| ispartof | Journal of neurochemistry, 1983-01, Vol.41 (5), p.1349-1356 |
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| subjects | 1-Methyl-3-isobutylxanthine - pharmacology 8-Bromo Cyclic Adenosine Monophosphate - pharmacology Animals Biological and medical sciences Bucladesine - pharmacology Cell physiology Choline O-Acetyltransferase - biosynthesis Cholinergic Cyclic AMP Cycloheximide - pharmacology Dactinomycin - pharmacology Development Enzyme Induction Female Fundamental and applied biological sciences. Psychology Induction Mice Molecular and cellular biology Neuron Neurotransmission Pregnancy Spinal cord Spinal Cord - drug effects Spinal Cord - enzymology Time Factors |
| title | Induction of Cholinergic Expression in Developing Spinal Cord Cultures |
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