Cyclohexanedione modification of arginine at the active site of Aspergillus ficuum phytase

Reaction of Aspergillus ficuum phytase with the arginine specific modifier 1,2-cyclohexanedione causes a rapid loss of activity. The inactivation can be partially reversed by 0.2 M hydroxylamine and exhibits pseudo-first order kinetics. The reaction order and second order rate constant of inactivati...

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Veröffentlicht in:	Biochemical and biophysical research communications 1991-07, Vol.178 (1), p.45-53
Hauptverfasser:	Ullah, A.H.J. (Southern Regional Research Center, ARS, USDA, New Orleans, LA), Cummins, B.J, Dischinger, H.C. Jr
Format:	Artikel
Sprache:	eng
Schlagworte:	1,2-cyclohexanedione 3-phytase 6-Phytase - antagonists & inhibitors 6-Phytase - genetics 6-Phytase - metabolism ACIDE AMINE ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Amino Acids AMINOACIDOS Analytical, structural and metabolic biochemistry ARGININA ARGININE ASPERGILLUS Aspergillus - enzymology Binding Sites Biological and medical sciences Chromatography, High Pressure Liquid Cyclohexanones - pharmacology Enzymes and enzyme inhibitors ESTERASAS ESTERASE Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Hydrolases Hydroxylamine Hydroxylamines - pharmacology INHIBIDORES DE ENZIMAS INHIBITEUR D'ENZYME Kinetics Molecular Sequence Data Peptide Fragments - isolation & purification Peptide Mapping Sequence Homology, Nucleic Acid Substrate Specificity
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