Desmin expression in fibroblasts of murine periovular granuloma during liver Schistosoma mansoni infection
We have studied the expression of the desmin gene, a muscle-specific intermediate filament protein in the granuloma cells of mouse liver infected with Schistosoma mansoni. In situ hybridization using a desmin DNA probe showed that fibroblastic cells in the granuloma strongly expressed desmin mRNAs,...
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| Veröffentlicht in: | Differentiation (London) 1991-03, Vol.46 (2), p.89-95 |
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| creator | Bolmont, Christine Andujar, Mauricio Peyrol, Simone Grimaud, Jean-Alexis |
| description | We have studied the expression of the desmin gene, a muscle-specific intermediate filament protein in the granuloma cells of mouse liver infected with
Schistosoma mansoni. In situ hybridization using a desmin DNA probe showed that fibroblastic cells in the granuloma strongly expressed desmin mRNAs, while in normal liver these cells did not express this mRNA to a detectable degree. The quantitative analysis of total RNAs demonstrated that the proportion of specific desmin mRNA increased from 14 to 18 weeks after infection and decreased at 20 weeks. The analysis of collagen gene expression indicated that the amount of type III collagen mRNAs was still increasing after 18 weeks from infection; in contrast, the amount of type I collagen mRNAs remained unchanged at that stage. A good correlation was observed between the detection of the specific mRNAs and the detection of both desmin and collagen molecules. Therefore, these data point to a coordinate induction of desmin and collagen gene expression during
Schistosomal granuloma formation. They also suggest that the expression of the myofibroblast phenotype involves the induction of both genes. |
| doi_str_mv | 10.1111/j.1432-0436.1991.tb00869.x |
| format | Article |
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Schistosoma mansoni. In situ hybridization using a desmin DNA probe showed that fibroblastic cells in the granuloma strongly expressed desmin mRNAs, while in normal liver these cells did not express this mRNA to a detectable degree. The quantitative analysis of total RNAs demonstrated that the proportion of specific desmin mRNA increased from 14 to 18 weeks after infection and decreased at 20 weeks. The analysis of collagen gene expression indicated that the amount of type III collagen mRNAs was still increasing after 18 weeks from infection; in contrast, the amount of type I collagen mRNAs remained unchanged at that stage. A good correlation was observed between the detection of the specific mRNAs and the detection of both desmin and collagen molecules. Therefore, these data point to a coordinate induction of desmin and collagen gene expression during
Schistosomal granuloma formation. They also suggest that the expression of the myofibroblast phenotype involves the induction of both genes.</description><identifier>ISSN: 0301-4681</identifier><identifier>EISSN: 1432-0436</identifier><identifier>DOI: 10.1111/j.1432-0436.1991.tb00869.x</identifier><identifier>PMID: 1906025</identifier><language>eng</language><publisher>Oxford, UK: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Blotting, Northern ; Desmin - biosynthesis ; Diseases caused by trematodes ; Female ; Fibroblasts - metabolism ; Fluorescent Antibody Technique ; Gene Expression Regulation ; Granuloma - metabolism ; Granuloma - pathology ; Helminthic diseases ; Infectious diseases ; Liver Diseases, Parasitic - metabolism ; Liver Diseases, Parasitic - pathology ; Medical sciences ; Mice ; Nucleic Acid Hybridization ; Parasitic diseases ; Schistosoma mansoni ; Schistosomiases ; Schistosomiasis mansoni - metabolism ; Schistosomiasis mansoni - pathology ; Tropical medicine</subject><ispartof>Differentiation (London), 1991-03, Vol.46 (2), p.89-95</ispartof><rights>1991 International Society of Differentiation</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4909-c3582adcadbb2c503212b6c13f06812375ebef3adef737157e013acbe6c5d34f3</citedby><cites>FETCH-LOGICAL-c4909-c3582adcadbb2c503212b6c13f06812375ebef3adef737157e013acbe6c5d34f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1111/j.1432-0436.1991.tb00869.x$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19661346$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1906025$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bolmont, Christine</creatorcontrib><creatorcontrib>Andujar, Mauricio</creatorcontrib><creatorcontrib>Peyrol, Simone</creatorcontrib><creatorcontrib>Grimaud, Jean-Alexis</creatorcontrib><title>Desmin expression in fibroblasts of murine periovular granuloma during liver Schistosoma mansoni infection</title><title>Differentiation (London)</title><addtitle>Differentiation</addtitle><description>We have studied the expression of the desmin gene, a muscle-specific intermediate filament protein in the granuloma cells of mouse liver infected with
Schistosoma mansoni. In situ hybridization using a desmin DNA probe showed that fibroblastic cells in the granuloma strongly expressed desmin mRNAs, while in normal liver these cells did not express this mRNA to a detectable degree. The quantitative analysis of total RNAs demonstrated that the proportion of specific desmin mRNA increased from 14 to 18 weeks after infection and decreased at 20 weeks. The analysis of collagen gene expression indicated that the amount of type III collagen mRNAs was still increasing after 18 weeks from infection; in contrast, the amount of type I collagen mRNAs remained unchanged at that stage. A good correlation was observed between the detection of the specific mRNAs and the detection of both desmin and collagen molecules. Therefore, these data point to a coordinate induction of desmin and collagen gene expression during
Schistosomal granuloma formation. They also suggest that the expression of the myofibroblast phenotype involves the induction of both genes.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Desmin - biosynthesis</subject><subject>Diseases caused by trematodes</subject><subject>Female</subject><subject>Fibroblasts - metabolism</subject><subject>Fluorescent Antibody Technique</subject><subject>Gene Expression Regulation</subject><subject>Granuloma - metabolism</subject><subject>Granuloma - pathology</subject><subject>Helminthic diseases</subject><subject>Infectious diseases</subject><subject>Liver Diseases, Parasitic - metabolism</subject><subject>Liver Diseases, Parasitic - pathology</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Nucleic Acid Hybridization</subject><subject>Parasitic diseases</subject><subject>Schistosoma mansoni</subject><subject>Schistosomiases</subject><subject>Schistosomiasis mansoni - metabolism</subject><subject>Schistosomiasis mansoni - pathology</subject><subject>Tropical medicine</subject><issn>0301-4681</issn><issn>1432-0436</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVUV2L1DAUDaKs4-pPEIqgb635aDON4IPsuLqw4IP6HJL0Zs3QNmNuO87-e1M6qG9qXkI4H7n3HEJeMFqxfF7vK1YLXtJayIopxarJUtpKVZ0ekM0v6CHZUEFZWcuWPSZPEPd0YXF2QS6YopLyZkP2O8AhjAWcDgkQQxyL_PLBpmh7gxMW0RfDnMIIxQFSiMe5N6m4S2ac-ziYoluwu6IPR0jFZ_ct4BRxAQYzYhxDtvPgpmz8lDzypkd4dr4vydfr91-uPpa3nz7cXL27LV2tqCqdaFpuOmc6a7lrqOCMW-mY8DQvwsW2AQtemA78VmxZswXKhHEWpGs6UXtxSV6tvocUv8-Akx4COuh7M0KcUbdUSsVE_Vcik5wqpdpMfLMSXYqICbw-pDCYdK8Z1Usjeq-X2PUSu14a0edG9CmLn59_me0A3W_pWkHGX55xg870PifrAv5BkzIPKzPv7cr7EXq4_48J9O7mulVZv1v1kLM_BkgaXYDRQRdSLkh3MfzLOj8B5JLAzw</recordid><startdate>199103</startdate><enddate>199103</enddate><creator>Bolmont, Christine</creator><creator>Andujar, Mauricio</creator><creator>Peyrol, Simone</creator><creator>Grimaud, Jean-Alexis</creator><general>Elsevier B.V</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>199103</creationdate><title>Desmin expression in fibroblasts of murine periovular granuloma during liver Schistosoma mansoni infection</title><author>Bolmont, Christine ; Andujar, Mauricio ; Peyrol, Simone ; Grimaud, Jean-Alexis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4909-c3582adcadbb2c503212b6c13f06812375ebef3adef737157e013acbe6c5d34f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Desmin - biosynthesis</topic><topic>Diseases caused by trematodes</topic><topic>Female</topic><topic>Fibroblasts - metabolism</topic><topic>Fluorescent Antibody Technique</topic><topic>Gene Expression Regulation</topic><topic>Granuloma - metabolism</topic><topic>Granuloma - pathology</topic><topic>Helminthic diseases</topic><topic>Infectious diseases</topic><topic>Liver Diseases, Parasitic - metabolism</topic><topic>Liver Diseases, Parasitic - pathology</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Nucleic Acid Hybridization</topic><topic>Parasitic diseases</topic><topic>Schistosoma mansoni</topic><topic>Schistosomiases</topic><topic>Schistosomiasis mansoni - metabolism</topic><topic>Schistosomiasis mansoni - pathology</topic><topic>Tropical medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bolmont, Christine</creatorcontrib><creatorcontrib>Andujar, Mauricio</creatorcontrib><creatorcontrib>Peyrol, Simone</creatorcontrib><creatorcontrib>Grimaud, Jean-Alexis</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Differentiation (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bolmont, Christine</au><au>Andujar, Mauricio</au><au>Peyrol, Simone</au><au>Grimaud, Jean-Alexis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Desmin expression in fibroblasts of murine periovular granuloma during liver Schistosoma mansoni infection</atitle><jtitle>Differentiation (London)</jtitle><addtitle>Differentiation</addtitle><date>1991-03</date><risdate>1991</risdate><volume>46</volume><issue>2</issue><spage>89</spage><epage>95</epage><pages>89-95</pages><issn>0301-4681</issn><eissn>1432-0436</eissn><abstract>We have studied the expression of the desmin gene, a muscle-specific intermediate filament protein in the granuloma cells of mouse liver infected with
Schistosoma mansoni. In situ hybridization using a desmin DNA probe showed that fibroblastic cells in the granuloma strongly expressed desmin mRNAs, while in normal liver these cells did not express this mRNA to a detectable degree. The quantitative analysis of total RNAs demonstrated that the proportion of specific desmin mRNA increased from 14 to 18 weeks after infection and decreased at 20 weeks. The analysis of collagen gene expression indicated that the amount of type III collagen mRNAs was still increasing after 18 weeks from infection; in contrast, the amount of type I collagen mRNAs remained unchanged at that stage. A good correlation was observed between the detection of the specific mRNAs and the detection of both desmin and collagen molecules. Therefore, these data point to a coordinate induction of desmin and collagen gene expression during
Schistosomal granuloma formation. They also suggest that the expression of the myofibroblast phenotype involves the induction of both genes.</abstract><cop>Oxford, UK</cop><pub>Elsevier B.V</pub><pmid>1906025</pmid><doi>10.1111/j.1432-0436.1991.tb00869.x</doi><tpages>7</tpages></addata></record> |
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| ispartof | Differentiation (London), 1991-03, Vol.46 (2), p.89-95 |
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| subjects | Animals Biological and medical sciences Blotting, Northern Desmin - biosynthesis Diseases caused by trematodes Female Fibroblasts - metabolism Fluorescent Antibody Technique Gene Expression Regulation Granuloma - metabolism Granuloma - pathology Helminthic diseases Infectious diseases Liver Diseases, Parasitic - metabolism Liver Diseases, Parasitic - pathology Medical sciences Mice Nucleic Acid Hybridization Parasitic diseases Schistosoma mansoni Schistosomiases Schistosomiasis mansoni - metabolism Schistosomiasis mansoni - pathology Tropical medicine |
| title | Desmin expression in fibroblasts of murine periovular granuloma during liver Schistosoma mansoni infection |
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