An indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo‐ and chlorodeoxyuridine incorporated into DNA

In this paper we describe an indirect fluorescence double staining procedure for the simultaneous detection of IdUrd and CldUrd in the same cell nucleus. Two commercially available antibodies were selected for this purpose. A rat antiBrdUrd monoclonal antibody from Seralab was found to bind specific...

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Veröffentlicht in:	Cytometry (New York, N.Y.) N.Y.), 1991, Vol.12 (4), p.366-372
Hauptverfasser:	Bakker, P. J. M., Stap, J., Tukker, C. J., van Oven, C. H., Veenhof, C. H. N., Aten, J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences cell kinetics Cells, Cultured CldUrd Cricetinae Cricetulus Deoxyuridine - analogs & derivatives Deoxyuridine - analysis Diverse techniques DNA - chemistry double staining procedure flow cytometry Flow Cytometry - methods Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Idoxuridine - analysis IdUrd Immunochemistry Mice Molecular and cellular biology
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