Immunohistochemical localization of alcohol dehydrogenase in the human gastrointestinal tract
To elucidate the possible role of alcohol dehydrogenase in the generation of damages caused by alcohol abuse, it is important to know the exact localization of this enzyme. Anti-alcohol dehydrogenase antibodies were used to localize the enzyme in human gastrointestinal tissues. Alcohol dehydrogenase...
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| Veröffentlicht in: | Gastroenterology (New York, N.Y. 1943) N.Y. 1943), 1983-11, Vol.85 (5), p.1011-1016 |
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| container_title | Gastroenterology (New York, N.Y. 1943) |
| container_volume | 85 |
| creator | PESTALOZZI, D. M BÜHLER, R VON WARTBURG, J. P HESS, M |
| description | To elucidate the possible role of alcohol dehydrogenase in the generation of damages caused by alcohol abuse, it is important to know the exact localization of this enzyme. Anti-alcohol dehydrogenase antibodies were used to localize the enzyme in human gastrointestinal tissues. Alcohol dehydrogenase was detectable in the mucosa of all parts of the gastrointestinal tract. There is a notable decrease in the amount of alcohol dehydrogenase with increasing distance from the stomach. In the stomach, the reaction for alcohol dehydrogenase was strongest in the mucus-producing cells, moderate in the parietal cells, and weak in the chief cells. Throughout the gut, alcohol dehydrogenase was present in cells exposed to the lumen in amounts comparable to that of the parietal cells. Crypt cells and goblet cells were negative. The results strongly indicate that alcohol dehydrogenase is an intrinsic component of gastrointestinal epithelial cells. |
| doi_str_mv | 10.1016/S0016-5085(83)80065-1 |
| format | Article |
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M</creatorcontrib><creatorcontrib>BÜHLER, R</creatorcontrib><creatorcontrib>VON WARTBURG, J. P</creatorcontrib><creatorcontrib>HESS, M</creatorcontrib><title>Immunohistochemical localization of alcohol dehydrogenase in the human gastrointestinal tract</title><title>Gastroenterology (New York, N.Y. 1943)</title><addtitle>Gastroenterology</addtitle><description>To elucidate the possible role of alcohol dehydrogenase in the generation of damages caused by alcohol abuse, it is important to know the exact localization of this enzyme. Anti-alcohol dehydrogenase antibodies were used to localize the enzyme in human gastrointestinal tissues. Alcohol dehydrogenase was detectable in the mucosa of all parts of the gastrointestinal tract. There is a notable decrease in the amount of alcohol dehydrogenase with increasing distance from the stomach. In the stomach, the reaction for alcohol dehydrogenase was strongest in the mucus-producing cells, moderate in the parietal cells, and weak in the chief cells. Throughout the gut, alcohol dehydrogenase was present in cells exposed to the lumen in amounts comparable to that of the parietal cells. Crypt cells and goblet cells were negative. The results strongly indicate that alcohol dehydrogenase is an intrinsic component of gastrointestinal epithelial cells.</description><subject>Alcohol Oxidoreductases - analysis</subject><subject>Alcohol Oxidoreductases - immunology</subject><subject>Alcoholism and acute alcohol poisoning</subject><subject>Antibodies</subject><subject>Biological and medical sciences</subject><subject>Colon - enzymology</subject><subject>Digestive System - enzymology</subject><subject>Epithelial Cells</subject><subject>Gastric Mucosa - enzymology</subject><subject>Humans</subject><subject>Immunochemistry</subject><subject>Intestinal Mucosa - enzymology</subject><subject>Intestine, Small - enzymology</subject><subject>Medical sciences</subject><subject>Rectum - enzymology</subject><subject>Staining and Labeling</subject><subject>Toxicology</subject><issn>0016-5085</issn><issn>1528-0012</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtLxTAQhYMoen38BKELEV1UJ22TpksRXyC4UJcS5qaJjbSJJulCf73xermLmVmcM3OGj5BjChcUKL98htxLBoKdifpcAHBW0i2yoKwSZdaqbbLYWPbIfowfANDVgu6SXU67Bmq-IG8P0zQ7P9iYvBr0ZBWOxehztz-YrHeFNwWOyg9-LHo9fPfBv2uHURfWFWnQxTBP6Ip3jCl465KOybp8IwVU6ZDsGByjPlrPA_J6e_NyfV8-Pt09XF89lqoWkEqjmAZT1wjCCNS5sEXOG6abtuoNAjU9KiZ40ytV0U6oVnUcWmOaJXQK6wNy-n_3M_ivOb8gJxuVHkd02s9RCuC8aps2G9m_UQUfY9BGfgY7YfiWFOQfVrnCKv-YSVHLFVZJ897xOmBeTrrfbK05Zv1krWPM7ExAp2zc2DrGOp7jfwFRJoLN</recordid><startdate>198311</startdate><enddate>198311</enddate><creator>PESTALOZZI, D. 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M</creatorcontrib><creatorcontrib>BÜHLER, R</creatorcontrib><creatorcontrib>VON WARTBURG, J. P</creatorcontrib><creatorcontrib>HESS, M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gastroenterology (New York, N.Y. 1943)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PESTALOZZI, D. M</au><au>BÜHLER, R</au><au>VON WARTBURG, J. 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| ispartof | Gastroenterology (New York, N.Y. 1943), 1983-11, Vol.85 (5), p.1011-1016 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier); Alma/SFX Local Collection |
| subjects | Alcohol Oxidoreductases - analysis Alcohol Oxidoreductases - immunology Alcoholism and acute alcohol poisoning Antibodies Biological and medical sciences Colon - enzymology Digestive System - enzymology Epithelial Cells Gastric Mucosa - enzymology Humans Immunochemistry Intestinal Mucosa - enzymology Intestine, Small - enzymology Medical sciences Rectum - enzymology Staining and Labeling Toxicology |
| title | Immunohistochemical localization of alcohol dehydrogenase in the human gastrointestinal tract |
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