Destabilization of the plasma membrane of isolated plant protoplasts during a freeze-thaw cycle: The influence of cold acclimation
In conclusion, isolated protoplasts are an excellent arena in which destabilization of the plasma membrane can be directly observed during a freeze-thaw cycle by cryomicroscopy. Destabilization is manifested in various ways—Intracellular ice formation, loss of osmotic responsiveness, or expansion-in...
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| Veröffentlicht in: | Cryobiology 1983-01, Vol.20 (4), p.448-465 |
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| container_title | Cryobiology |
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| creator | Steponkus, Peter L. Dowgert, Michael F. Gordon-Kamm, William J. |
| description | In conclusion, isolated protoplasts are an excellent arena in which destabilization of the plasma membrane can be directly observed during a freeze-thaw cycle by cryomicroscopy. Destabilization is manifested in various ways—Intracellular ice formation, loss of osmotic responsiveness, or expansion-induced lysis. The incidence of any particular form of injury will depend on the freeze-thaw protocol and hardiness of the tissue from which the protoplasts were isolated. In all cases, however, cold acclimation directly increases the stability of the plasma membrane to the multiple stresses that arise during a freeze-thaw cycle. Such observations provide for functional differences in the plasma membrane that may now be used to consider the significance of any compositional changes in the membrane that might be determined. |
| doi_str_mv | 10.1016/0011-2240(83)90035-4 |
| format | Article |
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Destabilization is manifested in various ways—Intracellular ice formation, loss of osmotic responsiveness, or expansion-induced lysis. The incidence of any particular form of injury will depend on the freeze-thaw protocol and hardiness of the tissue from which the protoplasts were isolated. In all cases, however, cold acclimation directly increases the stability of the plasma membrane to the multiple stresses that arise during a freeze-thaw cycle. Such observations provide for functional differences in the plasma membrane that may now be used to consider the significance of any compositional changes in the membrane that might be determined.</description><identifier>ISSN: 0011-2240</identifier><identifier>EISSN: 1090-2392</identifier><identifier>DOI: 10.1016/0011-2240(83)90035-4</identifier><identifier>PMID: 6617234</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Acclimatization ; Cell Membrane - physiology ; cold acclimation ; freeze-thawing ; Freezing ; Plant Physiological Phenomena ; plasma membranes ; protoplasts ; Protoplasts - physiology</subject><ispartof>Cryobiology, 1983-01, Vol.20 (4), p.448-465</ispartof><rights>1983</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c477t-9d1bee7dccebd0353efab1b04d2cce3e794a8b56dafbd4a3d2d57924b97fa2593</citedby><cites>FETCH-LOGICAL-c477t-9d1bee7dccebd0353efab1b04d2cce3e794a8b56dafbd4a3d2d57924b97fa2593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0011-2240(83)90035-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6617234$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Steponkus, Peter L.</creatorcontrib><creatorcontrib>Dowgert, Michael F.</creatorcontrib><creatorcontrib>Gordon-Kamm, William J.</creatorcontrib><title>Destabilization of the plasma membrane of isolated plant protoplasts during a freeze-thaw cycle: The influence of cold acclimation</title><title>Cryobiology</title><addtitle>Cryobiology</addtitle><description>In conclusion, isolated protoplasts are an excellent arena in which destabilization of the plasma membrane can be directly observed during a freeze-thaw cycle by cryomicroscopy. 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Such observations provide for functional differences in the plasma membrane that may now be used to consider the significance of any compositional changes in the membrane that might be determined.</description><subject>Acclimatization</subject><subject>Cell Membrane - physiology</subject><subject>cold acclimation</subject><subject>freeze-thawing</subject><subject>Freezing</subject><subject>Plant Physiological Phenomena</subject><subject>plasma membranes</subject><subject>protoplasts</subject><subject>Protoplasts - physiology</subject><issn>0011-2240</issn><issn>1090-2392</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctuFDEQRS1EFIbAHwDyCsGiwa9-mAUSCpAgRWJBsrb8qE6M3O3BdoOSZb4c98woS1hZqjp1XbcuQi8oeUcJ7d4TQmnDmCBvBv5WEsLbRjxCG0okaRiX7DHaPCBP0NOcfxJCup6LY3TcdbRnXGzQ_WfIRRsf_J0uPs44jrjcAN4GnSeNJ5hM0jOsZZ9j0AXc2psL3qZY4oqVjN2S_HyNNR4TwB005Ub_wfbWBviAL6uan8ewwGx3OjYGh7W1wU-7L5-ho1GHDM8P7wm6-vrl8vS8ufh-9u3000VjRd-XRjpqAHpnLRhXzXIYtaGGCMdqiUMvhR5M2zk9Gic0d8y1vWTCyH7UrJX8BL3e69bNfy3Vtpp8thCqG4hLVgOpV-Gk_S9I-cBaJkQFxR60KeacYFTbVE2lW0WJWjNSawBqDUANXO0yUuvYy4P-YiZwD0OHUGr_1b4_6qj0dfJZXf2gchCEtJS3rAIf9wDUc_32kFS2fj2v8wlsUS76f2_wF_0FrFo</recordid><startdate>19830101</startdate><enddate>19830101</enddate><creator>Steponkus, Peter L.</creator><creator>Dowgert, Michael F.</creator><creator>Gordon-Kamm, William J.</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19830101</creationdate><title>Destabilization of the plasma membrane of isolated plant protoplasts during a freeze-thaw cycle: The influence of cold acclimation</title><author>Steponkus, Peter L. ; Dowgert, Michael F. ; Gordon-Kamm, William J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c477t-9d1bee7dccebd0353efab1b04d2cce3e794a8b56dafbd4a3d2d57924b97fa2593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Acclimatization</topic><topic>Cell Membrane - physiology</topic><topic>cold acclimation</topic><topic>freeze-thawing</topic><topic>Freezing</topic><topic>Plant Physiological Phenomena</topic><topic>plasma membranes</topic><topic>protoplasts</topic><topic>Protoplasts - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Steponkus, Peter L.</creatorcontrib><creatorcontrib>Dowgert, Michael F.</creatorcontrib><creatorcontrib>Gordon-Kamm, William J.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cryobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Steponkus, Peter L.</au><au>Dowgert, Michael F.</au><au>Gordon-Kamm, William J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Destabilization of the plasma membrane of isolated plant protoplasts during a freeze-thaw cycle: The influence of cold acclimation</atitle><jtitle>Cryobiology</jtitle><addtitle>Cryobiology</addtitle><date>1983-01-01</date><risdate>1983</risdate><volume>20</volume><issue>4</issue><spage>448</spage><epage>465</epage><pages>448-465</pages><issn>0011-2240</issn><eissn>1090-2392</eissn><abstract>In conclusion, isolated protoplasts are an excellent arena in which destabilization of the plasma membrane can be directly observed during a freeze-thaw cycle by cryomicroscopy. Destabilization is manifested in various ways—Intracellular ice formation, loss of osmotic responsiveness, or expansion-induced lysis. The incidence of any particular form of injury will depend on the freeze-thaw protocol and hardiness of the tissue from which the protoplasts were isolated. In all cases, however, cold acclimation directly increases the stability of the plasma membrane to the multiple stresses that arise during a freeze-thaw cycle. Such observations provide for functional differences in the plasma membrane that may now be used to consider the significance of any compositional changes in the membrane that might be determined.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>6617234</pmid><doi>10.1016/0011-2240(83)90035-4</doi><tpages>18</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0011-2240 |
| ispartof | Cryobiology, 1983-01, Vol.20 (4), p.448-465 |
| issn | 0011-2240 1090-2392 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Acclimatization Cell Membrane - physiology cold acclimation freeze-thawing Freezing Plant Physiological Phenomena plasma membranes protoplasts Protoplasts - physiology |
| title | Destabilization of the plasma membrane of isolated plant protoplasts during a freeze-thaw cycle: The influence of cold acclimation |
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