Purification of hepatocyte couplets by centrifugal elutriation

An initial preparation of rat hepatocytes containing approximately 30% couplets was enriched by centrifugal elutriation. Of the couplets loaded onto the elutriator, 87% were eluted at medium flow rates of 60 to 80 ml/min at a rotor speed of 1,100 rpm; cells eluted in this range maintained a viabilit...

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Veröffentlicht in:	Hepatology (Baltimore, Md.) Md.), 1991-07, Vol.14 (1), p.180-183
Hauptverfasser:	Wilton, Joanne C., Williams, Delyth E., Strain, Alastair J., Parslow, Rosemary A., Chipman, J. Kevin, Coleman, Roger
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cytological Techniques Digestive system Fluorescein-5-isothiocyanate Fluoresceins Investigative techniques, diagnostic techniques (general aspects) Liver - cytology Liver - physiology Male Medical sciences Microscopy, Fluorescence Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Rats Rats, Inbred Strains Reproducibility of Results Thiocyanates
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