Purification of hepatocyte couplets by centrifugal elutriation
An initial preparation of rat hepatocytes containing approximately 30% couplets was enriched by centrifugal elutriation. Of the couplets loaded onto the elutriator, 87% were eluted at medium flow rates of 60 to 80 ml/min at a rotor speed of 1,100 rpm; cells eluted in this range maintained a viabilit...
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| Veröffentlicht in: | Hepatology (Baltimore, Md.) Md.), 1991-07, Vol.14 (1), p.180-183 |
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| container_title | Hepatology (Baltimore, Md.) |
| container_volume | 14 |
| creator | Wilton, Joanne C. Williams, Delyth E. Strain, Alastair J. Parslow, Rosemary A. Chipman, J. Kevin Coleman, Roger |
| description | An initial preparation of rat hepatocytes containing approximately 30% couplets was enriched by centrifugal elutriation. Of the couplets loaded onto the elutriator, 87% were eluted at medium flow rates of 60 to 80 ml/min at a rotor speed of 1,100 rpm; cells eluted in this range maintained a viability of more than 95%. Peak fractions were enriched in couplets to 84.5% ± 2.5%. After elutriation, couplets retained the ability to secrete fluorescent cholephiles into sealed canalicular vacuoles. The preparation can now be used in hepatobiliary and hepatotoxicity studies not possible with preparations in which they are minor components. (HEPATOLOGY 1991;14:180–183.) |
| doi_str_mv | 10.1002/hep.1840140129 |
| format | Article |
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Kevin</creatorcontrib><creatorcontrib>Coleman, Roger</creatorcontrib><title>Purification of hepatocyte couplets by centrifugal elutriation</title><title>Hepatology (Baltimore, Md.)</title><addtitle>Hepatology</addtitle><description>An initial preparation of rat hepatocytes containing approximately 30% couplets was enriched by centrifugal elutriation. Of the couplets loaded onto the elutriator, 87% were eluted at medium flow rates of 60 to 80 ml/min at a rotor speed of 1,100 rpm; cells eluted in this range maintained a viability of more than 95%. Peak fractions were enriched in couplets to 84.5% ± 2.5%. After elutriation, couplets retained the ability to secrete fluorescent cholephiles into sealed canalicular vacuoles. The preparation can now be used in hepatobiliary and hepatotoxicity studies not possible with preparations in which they are minor components. (HEPATOLOGY 1991;14:180–183.)</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cytological Techniques</subject><subject>Digestive system</subject><subject>Fluorescein-5-isothiocyanate</subject><subject>Fluoresceins</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Liver - cytology</subject><subject>Liver - physiology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microscopy, Fluorescence</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Reproducibility of Results</subject><subject>Thiocyanates</subject><issn>0270-9139</issn><issn>1527-3350</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM9LwzAUx4Moc06v3oQexFvny49myUWQMZ0wcIfdQ5ImWuna2rRI_3szN5w34cF75Pt53yRfhK4xTDEAuX93zRQLBjgWkSdojDMySynN4BSNgcwglZjKc3QRwgcASEbECI2wBA4sG6OHdd8WvrC6K-oqqX0S_XRX26Fzia37pnRdSMyQWFd1EezfdJm4so_zz8YlOvO6DO7q0Cdo87TYzJfp6vX5Zf64Si3lUqbMaywwt54YnOeGSptzMEwK0JZha6RxmSCgTTwQxDMaR4aZ4cL7nEk6QXd726atP3sXOrUtgnVlqStX90EJ4BwDpRGc7kHb1iG0zqumLba6HRQGtctLxf-pY15x4ebg3Juty4_4PqCo3x50HawufasrW4RfLMPAZbazkXvsqyjd8M-larlY_3nCN9Fsg_8</recordid><startdate>199107</startdate><enddate>199107</enddate><creator>Wilton, Joanne C.</creator><creator>Williams, Delyth E.</creator><creator>Strain, Alastair J.</creator><creator>Parslow, Rosemary A.</creator><creator>Chipman, J. 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Kevin ; Coleman, Roger</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3699-4fa1816cf2b1ddb39cd60b4980ac41cb9be5820ab80a82f430ab414b68ffd493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cytological Techniques</topic><topic>Digestive system</topic><topic>Fluorescein-5-isothiocyanate</topic><topic>Fluoresceins</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Liver - cytology</topic><topic>Liver - physiology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microscopy, Fluorescence</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Reproducibility of Results</topic><topic>Thiocyanates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wilton, Joanne C.</creatorcontrib><creatorcontrib>Williams, Delyth E.</creatorcontrib><creatorcontrib>Strain, Alastair J.</creatorcontrib><creatorcontrib>Parslow, Rosemary A.</creatorcontrib><creatorcontrib>Chipman, J. 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Kevin</au><au>Coleman, Roger</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification of hepatocyte couplets by centrifugal elutriation</atitle><jtitle>Hepatology (Baltimore, Md.)</jtitle><addtitle>Hepatology</addtitle><date>1991-07</date><risdate>1991</risdate><volume>14</volume><issue>1</issue><spage>180</spage><epage>183</epage><pages>180-183</pages><issn>0270-9139</issn><eissn>1527-3350</eissn><coden>HPTLD9</coden><abstract>An initial preparation of rat hepatocytes containing approximately 30% couplets was enriched by centrifugal elutriation. Of the couplets loaded onto the elutriator, 87% were eluted at medium flow rates of 60 to 80 ml/min at a rotor speed of 1,100 rpm; cells eluted in this range maintained a viability of more than 95%. Peak fractions were enriched in couplets to 84.5% ± 2.5%. After elutriation, couplets retained the ability to secrete fluorescent cholephiles into sealed canalicular vacuoles. 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| ispartof | Hepatology (Baltimore, Md.), 1991-07, Vol.14 (1), p.180-183 |
| issn | 0270-9139 1527-3350 |
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| source | Access via Wiley Online Library; MEDLINE |
| subjects | Animals Biological and medical sciences Cytological Techniques Digestive system Fluorescein-5-isothiocyanate Fluoresceins Investigative techniques, diagnostic techniques (general aspects) Liver - cytology Liver - physiology Male Medical sciences Microscopy, Fluorescence Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Rats Rats, Inbred Strains Reproducibility of Results Thiocyanates |
| title | Purification of hepatocyte couplets by centrifugal elutriation |
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