Phospholipase C activity in plasma membranes isolated from lapine synovial cells in monolayer culture
Plasma membranes were isolated from lapine synovial cells grown in monolayer culture using discontinuous sucrose gradient centrifugation techniques. 5′nucleotidase was detected in great abundance while glucose-6 phosphate dehydrogenase and cytochrome oxidase were present at low to undetectable level...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1983-08, Vol.115 (1), p.331-338 |
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| container_end_page | 338 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Rothenberg, R.J. Moskowitz, R.W. Malemud, C.J. |
| description | Plasma membranes were isolated from lapine synovial cells grown in monolayer culture using discontinuous sucrose gradient centrifugation techniques. 5′nucleotidase was detected in great abundance while glucose-6 phosphate dehydrogenase and cytochrome oxidase were present at low to undetectable levels. Plasma membranes incubated at 37°C for 60 min with [3H]-arachidonyl-phosphatidylinositol/phosphatidylserine synthesized [3H]-diacylglycerides. Little if any [3H]-diacylglyceride synthesis was measured when [3H]-arachidonyl-phosphatidylcholine or [3H]-arachidonyl-phosphatidylethanolamine were used as substrates. These results are consistent with a plasma membrane-associated phosphatidylinositol-specific phospholipase C from lapine synovial cells in culture. |
| doi_str_mv | 10.1016/0006-291X(83)91008-2 |
| format | Article |
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Plasma membranes incubated at 37°C for 60 min with [3H]-arachidonyl-phosphatidylinositol/phosphatidylserine synthesized [3H]-diacylglycerides. Little if any [3H]-diacylglyceride synthesis was measured when [3H]-arachidonyl-phosphatidylcholine or [3H]-arachidonyl-phosphatidylethanolamine were used as substrates. These results are consistent with a plasma membrane-associated phosphatidylinositol-specific phospholipase C from lapine synovial cells in culture.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(83)91008-2</identifier><identifier>PMID: 6615534</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cell Fractionation ; Cell Membrane - enzymology ; Cell Membrane - ultrastructure ; Cells, Cultured ; Centrifugation, Density Gradient ; Female ; Kinetics ; phospholipase C ; Phospholipases - metabolism ; Rabbits ; Synovial Membrane - enzymology ; synovium ; Type C Phospholipases - metabolism</subject><ispartof>Biochemical and biophysical research communications, 1983-08, Vol.115 (1), p.331-338</ispartof><rights>1983</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-ba3de37951c52133dca6b73591e8a3077ba8ee1e03bbdd41a2c663bf18b0b6713</citedby><cites>FETCH-LOGICAL-c388t-ba3de37951c52133dca6b73591e8a3077ba8ee1e03bbdd41a2c663bf18b0b6713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0006291X83910082$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6615534$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rothenberg, R.J.</creatorcontrib><creatorcontrib>Moskowitz, R.W.</creatorcontrib><creatorcontrib>Malemud, C.J.</creatorcontrib><title>Phospholipase C activity in plasma membranes isolated from lapine synovial cells in monolayer culture</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Plasma membranes were isolated from lapine synovial cells grown in monolayer culture using discontinuous sucrose gradient centrifugation techniques. 5′nucleotidase was detected in great abundance while glucose-6 phosphate dehydrogenase and cytochrome oxidase were present at low to undetectable levels. Plasma membranes incubated at 37°C for 60 min with [3H]-arachidonyl-phosphatidylinositol/phosphatidylserine synthesized [3H]-diacylglycerides. Little if any [3H]-diacylglyceride synthesis was measured when [3H]-arachidonyl-phosphatidylcholine or [3H]-arachidonyl-phosphatidylethanolamine were used as substrates. These results are consistent with a plasma membrane-associated phosphatidylinositol-specific phospholipase C from lapine synovial cells in culture.</description><subject>Animals</subject><subject>Cell Fractionation</subject><subject>Cell Membrane - enzymology</subject><subject>Cell Membrane - ultrastructure</subject><subject>Cells, Cultured</subject><subject>Centrifugation, Density Gradient</subject><subject>Female</subject><subject>Kinetics</subject><subject>phospholipase C</subject><subject>Phospholipases - metabolism</subject><subject>Rabbits</subject><subject>Synovial Membrane - enzymology</subject><subject>synovium</subject><subject>Type C Phospholipases - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFr3DAQhUVpSTdp_kELOpXm4HbGsmX5UihLkxQC7SGB3oQkzxIV2XIle2H_fe3skmNzmsO892b4HmPvET4joPwCALIoW_z9SYmrFgFUUb5iG4QWihKhes02z5K37DznPwCIlWzP2JmUWNei2jD69Rjz-BiDH00mvuXGTX7vpwP3Ax-Dyb3hPfU2mYEy9zkGM1HHdyn2PJjRD8TzYYh7bwJ3FEJefX0cFt2BEndzmOZE79ibnQmZLk_zgj1cf7_f3hZ3P29-bL_dFU4oNRXWiI5E09bo6hKF6JyRthF1i6SMgKaxRhEhgbC26yo0pZNS2B0qC1Y2KC7Yx2PumOLfmfKke5_Xt5bv45y1AlkrIeSLQqwWUEKtidVR6FLMOdFOj8n3Jh00gl5r0CtjvTLWSuinGnS52D6c8mfbU_dsOnFf9l-Pe1po7D0lnZ2nwVHnE7lJd9H__8A_vtGYlA</recordid><startdate>19830830</startdate><enddate>19830830</enddate><creator>Rothenberg, R.J.</creator><creator>Moskowitz, R.W.</creator><creator>Malemud, C.J.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19830830</creationdate><title>Phospholipase C activity in plasma membranes isolated from lapine synovial cells in monolayer culture</title><author>Rothenberg, R.J. ; Moskowitz, R.W. ; Malemud, C.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-ba3de37951c52133dca6b73591e8a3077ba8ee1e03bbdd41a2c663bf18b0b6713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Animals</topic><topic>Cell Fractionation</topic><topic>Cell Membrane - enzymology</topic><topic>Cell Membrane - ultrastructure</topic><topic>Cells, Cultured</topic><topic>Centrifugation, Density Gradient</topic><topic>Female</topic><topic>Kinetics</topic><topic>phospholipase C</topic><topic>Phospholipases - metabolism</topic><topic>Rabbits</topic><topic>Synovial Membrane - enzymology</topic><topic>synovium</topic><topic>Type C Phospholipases - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rothenberg, R.J.</creatorcontrib><creatorcontrib>Moskowitz, R.W.</creatorcontrib><creatorcontrib>Malemud, C.J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rothenberg, R.J.</au><au>Moskowitz, R.W.</au><au>Malemud, C.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phospholipase C activity in plasma membranes isolated from lapine synovial cells in monolayer culture</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1983-08-30</date><risdate>1983</risdate><volume>115</volume><issue>1</issue><spage>331</spage><epage>338</epage><pages>331-338</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Plasma membranes were isolated from lapine synovial cells grown in monolayer culture using discontinuous sucrose gradient centrifugation techniques. 5′nucleotidase was detected in great abundance while glucose-6 phosphate dehydrogenase and cytochrome oxidase were present at low to undetectable levels. Plasma membranes incubated at 37°C for 60 min with [3H]-arachidonyl-phosphatidylinositol/phosphatidylserine synthesized [3H]-diacylglycerides. Little if any [3H]-diacylglyceride synthesis was measured when [3H]-arachidonyl-phosphatidylcholine or [3H]-arachidonyl-phosphatidylethanolamine were used as substrates. These results are consistent with a plasma membrane-associated phosphatidylinositol-specific phospholipase C from lapine synovial cells in culture.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6615534</pmid><doi>10.1016/0006-291X(83)91008-2</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1983-08, Vol.115 (1), p.331-338 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Cell Fractionation Cell Membrane - enzymology Cell Membrane - ultrastructure Cells, Cultured Centrifugation, Density Gradient Female Kinetics phospholipase C Phospholipases - metabolism Rabbits Synovial Membrane - enzymology synovium Type C Phospholipases - metabolism |
| title | Phospholipase C activity in plasma membranes isolated from lapine synovial cells in monolayer culture |
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