Fracture healing induces expression of the proto-oncogene c-fos in vivo Possible involvement of the Fos protein in osteoblastic differentiation

Here we report marked in vivo expression of the c-fos gene in the external soft callus (ESC) and periosteal hard callus (PHC) at the fracture site of adult rat tibia. Northern-blot analysis showed that the ESC expressed a high level of c-fos mRNA from post-fracture day 10 to day 28, the time when en...

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Veröffentlicht in:FEBS letters 1991-06, Vol.284 (1), p.42-45
Hauptverfasser: Ohta, Shuichi, Yamamuro, Takao, Lee, Kaechoong, Okumura, Hideo, Kasai, Ryuichi, Hiraki, Yuji, Ikeda, Toshihiko, Iwasaki, Rempei, Kikuchi, Haruki, Konishi, Junji, Shigeno, Chohei
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Sprache:eng
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Zusammenfassung:Here we report marked in vivo expression of the c-fos gene in the external soft callus (ESC) and periosteal hard callus (PHC) at the fracture site of adult rat tibia. Northern-blot analysis showed that the ESC expressed a high level of c-fos mRNA from post-fracture day 10 to day 28, the time when endochondral ossification progressed, and that the ossifying PHC also expressed c-fos mRNA. This c-fos expression was followed by sequential expression of the genes for alkaline phosphatase, osteopontin and osteocalcin, which are osteoblastic markers. Immunohistochemical analysis showed that the c-Fos protein was predominantly located in osteoblasts in the ossifying calluses.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(91)80757-T