A Study of the Microcirculation in Whole Villi of Neonatal Mice Using a Peroxidase Histochemical Staining Method
The anatomy of the microcirculation of intestinal villi from the upper, middle, and lower small intestine of neonatal mice from 8 to 14 days old was studied using a histochemical peroxidase technique that specifically stained erythrocytes. Over 8–14 days, there was little chronological variation bet...
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| Veröffentlicht in: | Journal of pediatric gastroenterology and nutrition 1991-01, Vol.12 (1), p.105-110 |
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| container_title | Journal of pediatric gastroenterology and nutrition |
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| creator | Osborne, Michael P Haddon, Susan J Worton, Kim J Spencer, Anthony J Starkey, William G Thornber, Dawn Stephen, John |
| description | The anatomy of the microcirculation of intestinal villi from the upper, middle, and lower small intestine of neonatal mice from 8 to 14 days old was studied using a histochemical peroxidase technique that specifically stained erythrocytes. Over 8–14 days, there was little chronological variation between the same regions of gut; the exception was the lower intestine, which, in younger mice, was noticeably less well perfused with erythrocytes. Vascular beds in the middle and lower intestine comprised a hairpin loop with cross-connections. In the upper intestine, the capillary beds were generally more complex, particularly in apical regions of the villi. Most villi were well perfused with erythrocytes, but a minority ( |
| doi_str_mv | 10.1097/00005176-199101000-00020 |
| format | Article |
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Over 8–14 days, there was little chronological variation between the same regions of gut; the exception was the lower intestine, which, in younger mice, was noticeably less well perfused with erythrocytes. Vascular beds in the middle and lower intestine comprised a hairpin loop with cross-connections. In the upper intestine, the capillary beds were generally more complex, particularly in apical regions of the villi. Most villi were well perfused with erythrocytes, but a minority (<10%) contained considerably fewer red cells, some to the point of being totally ischemic. Other villi (<5%) were hyperemic, and the vascular beds packed and engorged with red cells. Usually, the packing density (hematocrit) of red cells within blood vessels increased progressively from villus base to apex. Red-cell deformation was more prevalent at villus apices, with marked crenation in some villi, yet in the basal regions of these same villi, the red cells were of normal discoid shape. The peroxidase staining technique produces a reliable histological picture of red cells circulating through villi. It also reveals differential perfusion of erythrocytes between and within villi, and that blood vessels pass through hypertonic zones in the apical regions of villi.</description><identifier>ISSN: 0277-2116</identifier><identifier>EISSN: 1536-4801</identifier><identifier>DOI: 10.1097/00005176-199101000-00020</identifier><identifier>PMID: 2061765</identifier><identifier>CODEN: JPGND6</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott-Raven Publishers</publisher><subject>Animals ; Animals, Newborn - physiology ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Hematocrit ; Immunoenzyme Techniques ; Intestinal Mucosa - blood supply ; Intestinal Mucosa - ultrastructure ; Intestine, Small - blood supply ; Intestine. Mesentery ; Mice ; Mice, Inbred BALB C ; Microcirculation ; Microscopy, Electron ; Microvilli ; Vertebrates: digestive system</subject><ispartof>Journal of pediatric gastroenterology and nutrition, 1991-01, Vol.12 (1), p.105-110</ispartof><rights>Lippincott-Raven Publishers.</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4350-6efe9613aa1c5bf1722a5629ea5d78ba708ce23a9da2ec4c23ced24b01a8498b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19652747$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2061765$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Osborne, Michael P</creatorcontrib><creatorcontrib>Haddon, Susan J</creatorcontrib><creatorcontrib>Worton, Kim J</creatorcontrib><creatorcontrib>Spencer, Anthony J</creatorcontrib><creatorcontrib>Starkey, William G</creatorcontrib><creatorcontrib>Thornber, Dawn</creatorcontrib><creatorcontrib>Stephen, John</creatorcontrib><title>A Study of the Microcirculation in Whole Villi of Neonatal Mice Using a Peroxidase Histochemical Staining Method</title><title>Journal of pediatric gastroenterology and nutrition</title><addtitle>J Pediatr Gastroenterol Nutr</addtitle><description>The anatomy of the microcirculation of intestinal villi from the upper, middle, and lower small intestine of neonatal mice from 8 to 14 days old was studied using a histochemical peroxidase technique that specifically stained erythrocytes. Over 8–14 days, there was little chronological variation between the same regions of gut; the exception was the lower intestine, which, in younger mice, was noticeably less well perfused with erythrocytes. Vascular beds in the middle and lower intestine comprised a hairpin loop with cross-connections. In the upper intestine, the capillary beds were generally more complex, particularly in apical regions of the villi. Most villi were well perfused with erythrocytes, but a minority (<10%) contained considerably fewer red cells, some to the point of being totally ischemic. Other villi (<5%) were hyperemic, and the vascular beds packed and engorged with red cells. Usually, the packing density (hematocrit) of red cells within blood vessels increased progressively from villus base to apex. Red-cell deformation was more prevalent at villus apices, with marked crenation in some villi, yet in the basal regions of these same villi, the red cells were of normal discoid shape. The peroxidase staining technique produces a reliable histological picture of red cells circulating through villi. It also reveals differential perfusion of erythrocytes between and within villi, and that blood vessels pass through hypertonic zones in the apical regions of villi.</description><subject>Animals</subject><subject>Animals, Newborn - physiology</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hematocrit</subject><subject>Immunoenzyme Techniques</subject><subject>Intestinal Mucosa - blood supply</subject><subject>Intestinal Mucosa - ultrastructure</subject><subject>Intestine, Small - blood supply</subject><subject>Intestine. Mesentery</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microcirculation</subject><subject>Microscopy, Electron</subject><subject>Microvilli</subject><subject>Vertebrates: digestive system</subject><issn>0277-2116</issn><issn>1536-4801</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kV1vFCEUhonR1LX6E0y40btRYPiYuWwatSatmtTqJTnDnHFQdliBSe2_l3XXeiUJIeQ87znhgRDK2SvOevOa1aW40Q3ve854vTV1C_aAbLhqdSM7xh-SDRPGNIJz_Zg8yfl7RYxU7IScCKZrWm3I7oxel3W8o3GiZUZ65V2Kzie3Big-LtQv9OscA9IvPgS_xz5gXKBA2LNIb7JfvlGgnzDFX36EjPTC5xLdjFvvKnVdwC975grLHMen5NEEIeOz43lKbt6--Xx-0Vx-fPf-_OyycbJVrNE4Ya95C8CdGiZuhAClRY-gRtMNYFjnULTQjyDQSSdah6OQA-PQyb4b2lPy8tB3l-LPFXOxW58dhgALxjXbjmklBRcV7A5gfXjOCSe7S34L6c5yZvey7V_Z9l62_SO7Rp8fZ6zDFsf74NFurb841iFXFVOCxfn8r3-vlTDSVE4euNsYCqb8I6y3mOyMEMps__fX7W-MZJbl</recordid><startdate>199101</startdate><enddate>199101</enddate><creator>Osborne, Michael P</creator><creator>Haddon, Susan J</creator><creator>Worton, Kim J</creator><creator>Spencer, Anthony J</creator><creator>Starkey, William G</creator><creator>Thornber, Dawn</creator><creator>Stephen, John</creator><general>Lippincott-Raven Publishers</general><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199101</creationdate><title>A Study of the Microcirculation in Whole Villi of Neonatal Mice Using a Peroxidase Histochemical Staining Method</title><author>Osborne, Michael P ; Haddon, Susan J ; Worton, Kim J ; Spencer, Anthony J ; Starkey, William G ; Thornber, Dawn ; Stephen, John</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4350-6efe9613aa1c5bf1722a5629ea5d78ba708ce23a9da2ec4c23ced24b01a8498b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Animals, Newborn - physiology</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hematocrit</topic><topic>Immunoenzyme Techniques</topic><topic>Intestinal Mucosa - blood supply</topic><topic>Intestinal Mucosa - ultrastructure</topic><topic>Intestine, Small - blood supply</topic><topic>Intestine. Mesentery</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microcirculation</topic><topic>Microscopy, Electron</topic><topic>Microvilli</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Osborne, Michael P</creatorcontrib><creatorcontrib>Haddon, Susan J</creatorcontrib><creatorcontrib>Worton, Kim J</creatorcontrib><creatorcontrib>Spencer, Anthony J</creatorcontrib><creatorcontrib>Starkey, William G</creatorcontrib><creatorcontrib>Thornber, Dawn</creatorcontrib><creatorcontrib>Stephen, John</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pediatric gastroenterology and nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Osborne, Michael P</au><au>Haddon, Susan J</au><au>Worton, Kim J</au><au>Spencer, Anthony J</au><au>Starkey, William G</au><au>Thornber, Dawn</au><au>Stephen, John</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Study of the Microcirculation in Whole Villi of Neonatal Mice Using a Peroxidase Histochemical Staining Method</atitle><jtitle>Journal of pediatric gastroenterology and nutrition</jtitle><addtitle>J Pediatr Gastroenterol Nutr</addtitle><date>1991-01</date><risdate>1991</risdate><volume>12</volume><issue>1</issue><spage>105</spage><epage>110</epage><pages>105-110</pages><issn>0277-2116</issn><eissn>1536-4801</eissn><coden>JPGND6</coden><abstract>The anatomy of the microcirculation of intestinal villi from the upper, middle, and lower small intestine of neonatal mice from 8 to 14 days old was studied using a histochemical peroxidase technique that specifically stained erythrocytes. Over 8–14 days, there was little chronological variation between the same regions of gut; the exception was the lower intestine, which, in younger mice, was noticeably less well perfused with erythrocytes. Vascular beds in the middle and lower intestine comprised a hairpin loop with cross-connections. In the upper intestine, the capillary beds were generally more complex, particularly in apical regions of the villi. Most villi were well perfused with erythrocytes, but a minority (<10%) contained considerably fewer red cells, some to the point of being totally ischemic. Other villi (<5%) were hyperemic, and the vascular beds packed and engorged with red cells. Usually, the packing density (hematocrit) of red cells within blood vessels increased progressively from villus base to apex. Red-cell deformation was more prevalent at villus apices, with marked crenation in some villi, yet in the basal regions of these same villi, the red cells were of normal discoid shape. 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| ispartof | Journal of pediatric gastroenterology and nutrition, 1991-01, Vol.12 (1), p.105-110 |
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| source | MEDLINE; Journals@Ovid Complete |
| subjects | Animals Animals, Newborn - physiology Biological and medical sciences Fundamental and applied biological sciences. Psychology Hematocrit Immunoenzyme Techniques Intestinal Mucosa - blood supply Intestinal Mucosa - ultrastructure Intestine, Small - blood supply Intestine. Mesentery Mice Mice, Inbred BALB C Microcirculation Microscopy, Electron Microvilli Vertebrates: digestive system |
| title | A Study of the Microcirculation in Whole Villi of Neonatal Mice Using a Peroxidase Histochemical Staining Method |
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