An ELISA for Pregnancy-Associated Plasma Protein A
An enzyme-linked immunoassay for pregnancy-associated plasma protein A is described. The assay is sensitive (7·6 IU/1) and precise (overall CV varied between 8·9% and 10·8%). Recovery was 100% over the working range. Parallel behaviour was observed between the International Reference Preparation 78/...
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| Veröffentlicht in: | Annals of Clinical Biochemistry 1983-07, Vol.20 (4), p.236-240 |
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| container_title | Annals of Clinical Biochemistry |
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| creator | Pledger, D R Belfield, A |
| description | An enzyme-linked immunoassay for pregnancy-associated plasma protein A is described. The assay is sensitive (7·6 IU/1) and precise (overall CV varied between 8·9% and 10·8%). Recovery was 100% over the working range. Parallel behaviour was observed between the International Reference Preparation 78/610 and pregnancy serum, thus allowing calibration of a laboratory standard pool against IRP 78/610. |
| doi_str_mv | 10.1177/000456328302000410 |
| format | Article |
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The assay is sensitive (7·6 IU/1) and precise (overall CV varied between 8·9% and 10·8%). Recovery was 100% over the working range. Parallel behaviour was observed between the International Reference Preparation 78/610 and pregnancy serum, thus allowing calibration of a laboratory standard pool against IRP 78/610.</description><identifier>ISSN: 0004-5632</identifier><identifier>EISSN: 1758-1001</identifier><identifier>DOI: 10.1177/000456328302000410</identifier><identifier>PMID: 6193752</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Antigen-Antibody Complex ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Microchemistry ; Pregnancy ; Pregnancy Proteins - analysis ; Pregnancy-Associated Plasma Protein-A - analysis</subject><ispartof>Annals of Clinical Biochemistry, 1983-07, Vol.20 (4), p.236-240</ispartof><rights>1983 Association for Clinical Biochemistry</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-ac79bb1b25d238cee9f87923d7695546ee23dabe905cd72d949e5b5607c1c4dc3</citedby><cites>FETCH-LOGICAL-c413t-ac79bb1b25d238cee9f87923d7695546ee23dabe905cd72d949e5b5607c1c4dc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>313,314,780,784,792,27922,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6193752$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pledger, D R</creatorcontrib><creatorcontrib>Belfield, A</creatorcontrib><title>An ELISA for Pregnancy-Associated Plasma Protein A</title><title>Annals of Clinical Biochemistry</title><addtitle>Ann Clin Biochem</addtitle><description>An enzyme-linked immunoassay for pregnancy-associated plasma protein A is described. The assay is sensitive (7·6 IU/1) and precise (overall CV varied between 8·9% and 10·8%). Recovery was 100% over the working range. Parallel behaviour was observed between the International Reference Preparation 78/610 and pregnancy serum, thus allowing calibration of a laboratory standard pool against IRP 78/610.</description><subject>Antigen-Antibody Complex</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Humans</subject><subject>Microchemistry</subject><subject>Pregnancy</subject><subject>Pregnancy Proteins - analysis</subject><subject>Pregnancy-Associated Plasma Protein-A - analysis</subject><issn>0004-5632</issn><issn>1758-1001</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9Lw0AQxRdRaq1-AUHIyVvs7G52N3sMpWqhYEE9h81mUlLyp-4mh357Exq8CHqaN_PevMOPkHsKT5QqtQSASEjOYg5s1BQuyJwqEYcUgF6S-XgMx8Q1ufH-MKxMAczITFLNlWBzwpImWG8370lQtC7YOdw3prGnMPG-taXpMA92lfG1Gby2w7IJkltyVZjK4900F-Tzef2xeg23by-bVbINbUR5FxqrdJbRjImc8dgi6iJWmvFcSS1EJBEHbTLUIGyuWK4jjSITEpSlNsotX5DHc-_RtV89-i6tS2-xqkyDbe_TGGQUa6X-DVKulQQhhiA7B61rvXdYpEdX1sadUgrpSDT9TXR4epja-6zG_OdlQjj4y7PvzR7TQ9u7ZqDyV-M3D8Z7Hg</recordid><startdate>198307</startdate><enddate>198307</enddate><creator>Pledger, D R</creator><creator>Belfield, A</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>198307</creationdate><title>An ELISA for Pregnancy-Associated Plasma Protein A</title><author>Pledger, D R ; Belfield, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-ac79bb1b25d238cee9f87923d7695546ee23dabe905cd72d949e5b5607c1c4dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Antigen-Antibody Complex</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Humans</topic><topic>Microchemistry</topic><topic>Pregnancy</topic><topic>Pregnancy Proteins - analysis</topic><topic>Pregnancy-Associated Plasma Protein-A - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pledger, D R</creatorcontrib><creatorcontrib>Belfield, A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of Clinical Biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pledger, D R</au><au>Belfield, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An ELISA for Pregnancy-Associated Plasma Protein A</atitle><jtitle>Annals of Clinical Biochemistry</jtitle><addtitle>Ann Clin Biochem</addtitle><date>1983-07</date><risdate>1983</risdate><volume>20</volume><issue>4</issue><spage>236</spage><epage>240</epage><pages>236-240</pages><issn>0004-5632</issn><eissn>1758-1001</eissn><abstract>An enzyme-linked immunoassay for pregnancy-associated plasma protein A is described. The assay is sensitive (7·6 IU/1) and precise (overall CV varied between 8·9% and 10·8%). Recovery was 100% over the working range. Parallel behaviour was observed between the International Reference Preparation 78/610 and pregnancy serum, thus allowing calibration of a laboratory standard pool against IRP 78/610.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>6193752</pmid><doi>10.1177/000456328302000410</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0004-5632 |
| ispartof | Annals of Clinical Biochemistry, 1983-07, Vol.20 (4), p.236-240 |
| issn | 0004-5632 1758-1001 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80648977 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Antigen-Antibody Complex Enzyme-Linked Immunosorbent Assay Female Humans Microchemistry Pregnancy Pregnancy Proteins - analysis Pregnancy-Associated Plasma Protein-A - analysis |
| title | An ELISA for Pregnancy-Associated Plasma Protein A |
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