Determination of human serum lactate dehydrogenase isoenzymes by anion exchange chromatography
A pH-coupled salt gradient elution technique has been developed for the complete frationation of lactate dehydrogenase (LDH) isoenzymes in human sera by using a miniature polystyrene column packed with preequilibrated DEAE-cellulose to a bed of 8 cm x 0.7 cm. Five different buffers containing 0.02 M...
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| Veröffentlicht in: | Analytical chemistry (Washington) 1983-07, Vol.55 (8), p.1385-1390 |
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| container_title | Analytical chemistry (Washington) |
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| creator | Menon, M. P Anderson, Geraldine Nambiar, G. K |
| description | A pH-coupled salt gradient elution technique has been developed for the complete frationation of lactate dehydrogenase (LDH) isoenzymes in human sera by using a miniature polystyrene column packed with preequilibrated DEAE-cellulose to a bed of 8 cm x 0.7 cm. Five different buffers containing 0.02 M Tris (tris(hydroxymethyl)aminomethane hydrochloride) with increasing salt concentration and decreasing pH were used to separate the isoenzymes from 0.3 to 0.5 mL of sera absorbed on the column. |
| doi_str_mv | 10.1021/ac00259a044 |
| format | Article |
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Five different buffers containing 0.02 M Tris (tris(hydroxymethyl)aminomethane hydrochloride) with increasing salt concentration and decreasing pH were used to separate the isoenzymes from 0.3 to 0.5 mL of sera absorbed on the column.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac00259a044</identifier><identifier>PMID: 6614492</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>anion-exchange chromatography ; Chromatography, DEAE-Cellulose - methods ; Humans ; Isoenzymes ; L-Lactate Dehydrogenase - blood ; L-Lactate Dehydrogenase - isolation & purification ; lactate dehydrogenase ; man ; serum</subject><ispartof>Analytical chemistry (Washington), 1983-07, Vol.55 (8), p.1385-1390</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a385t-94dd61ff3e61ab94c9b8527afcb7a07db7df6f1194ef0127da3474cedd0c81453</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac00259a044$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac00259a044$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2763,27075,27923,27924,56737,56787</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6614492$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Menon, M. 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Five different buffers containing 0.02 M Tris (tris(hydroxymethyl)aminomethane hydrochloride) with increasing salt concentration and decreasing pH were used to separate the isoenzymes from 0.3 to 0.5 mL of sera absorbed on the column.</description><subject>anion-exchange chromatography</subject><subject>Chromatography, DEAE-Cellulose - methods</subject><subject>Humans</subject><subject>Isoenzymes</subject><subject>L-Lactate Dehydrogenase - blood</subject><subject>L-Lactate Dehydrogenase - isolation & purification</subject><subject>lactate dehydrogenase</subject><subject>man</subject><subject>serum</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFv1DAQhS1EVbaFE2ckn-CAUsaOEydHtNCCWokKyhVrYk82KRt7aydSw68n1a6qHpA4zeF97430MfZawJkAKT6gBZBFjaDUM7YShYSsrCr5nK0AIM-kBnjBTlK6BRACRHnMjstSKFXLFfv1iUaKQ-9x7IPnoeXdNKDnieI08C3aEUfijrrZxbAhj4l4nwL5P_NAiTczR_9QpHvbod8Qt10MA45hE3HXzS_ZUYvbRK8O95T9PP98s_6SXX27-Lr-eJVhXhVjVivnStG2OZUCm1rZuqkKqbG1jUbQrtGuLVshakUtCKkd5korS86BrYQq8lP2dr-7i-FuojSaoU-Wtlv0FKZkKiiVXjr_BUVeSbkoXMD3e9DGkFKk1uxiP2CcjQDzoN080b7Qbw6zUzOQe2QPnpc82-d9Gun-Mcb425Q614W5uf5hLutiff1daHO-8O_2PNpkbsMU_WLvn5__At8Smmc</recordid><startdate>198307</startdate><enddate>198307</enddate><creator>Menon, M. 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| ispartof | Analytical chemistry (Washington), 1983-07, Vol.55 (8), p.1385-1390 |
| issn | 0003-2700 1520-6882 |
| language | eng |
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| source | MEDLINE; ACS Publications |
| subjects | anion-exchange chromatography Chromatography, DEAE-Cellulose - methods Humans Isoenzymes L-Lactate Dehydrogenase - blood L-Lactate Dehydrogenase - isolation & purification lactate dehydrogenase man serum |
| title | Determination of human serum lactate dehydrogenase isoenzymes by anion exchange chromatography |
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