Antigens in perienteric fluid of Ascaris suum as detected through antibodies in pigs orally inoculated with fully embryonated eggs
Perienteric fluid (Pf) of adult Ascaris suum was fractionated by ammonium sulfate precipitation, gel filtration or DEAE-cellulose chromatography, and sucrose density gradient centrifugation. Anti-sera (anti-EE) from pigs which were inoculated orally with fully embryonated eggs (EE) of A. suum were u...
Gespeichert in:
| Veröffentlicht in: | Veterinary parasitology 1983-01, Vol.12 (2), p.179-186 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 186 |
|---|---|
| container_issue | 2 |
| container_start_page | 179 |
| container_title | Veterinary parasitology |
| container_volume | 12 |
| creator | Rhodes, M.B. Staudinger, Lisa A. |
| description | Perienteric fluid (Pf) of adult
Ascaris suum was fractionated by ammonium sulfate precipitation, gel filtration or DEAE-cellulose chromatography, and sucrose density gradient centrifugation. Anti-sera (anti-EE) from pigs which were inoculated orally with fully embryonated eggs (EE) of
A. suum were used in an indirect radioimmunoassay (IRIA) to determine which fractions of Pf reacted positively in the analyses at the lowest protein concentration. These fractions were considered to contain more potent antigens. Comparative IRIA were performed employing antisera (anti-Pf) produced by injecting Pf into pigs. Six out of 35 fractions reacted positively at ⩽ 0.2
μg protein when anti-EE was used in the IRIA. Twenty-two out of 35 fractions reacted positively at ⩽ 0.2
μg protein when anti-Pf was used. Five of the 6 fractions reacting positively with anti-EE also reacted with anti-Pf. A 76 000 dalton component appears to be one of the major proteins in the 6 fractions which react positively with anti-EE while components from 10 000–138 000 daltons were present in the various fractions reacting positively with anti-Pf at ⩽ 0.2
μg protein. |
| doi_str_mv | 10.1016/0304-4017(83)90006-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_80639099</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0304401783900067</els_id><sourcerecordid>80639099</sourcerecordid><originalsourceid>FETCH-LOGICAL-c388t-8e2271ae11715a80126f12f745525a18df341122c13ab7739515cd92566443f63</originalsourceid><addsrcrecordid>eNqFkU1v1DAQhi1EVZbCPwDJJwSHFE_8mQvSquJLqtQLnC2vM8kaJfFiJ1R75ZfX6a56hIstzzzzWpqHkDfAroGB-sg4E5VgoN8b_qFhjKlKPyMbMJpXtZTsOdk8IS_Iy5x_FUYwpS_JpVJGcMk35O92mkOPU6ZhogdMAae5nJ52wxJaGju6zd6lkGlelpG6TFuc0c_Y0nmf4tLvqSsJu9gGPGWEPtOY3DAcyzP6ZXArfB_mPe2WtYrjLh3j9FjGvs-vyEXnhoyvz_cV-fnl84-bb9Xt3dfvN9vbynNj5spgXWtwCKBBOsOgVh3UnRZS1tKBaTsuAOraA3c7rXkjQfq2qaVSQvBO8Svy7pR7SPH3gnm2Y8geh8FNGJdsDVO8YU3zXxC4FkaDKaA4gT7FnBN29pDC6NLRArOrI7sKsKsAa7h9dGR1GXt7zl92I7ZPQ2cppf_p1MeyjT8Bk82-ePHYhlRWb9sY_v3BA7r-oMw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>13748718</pqid></control><display><type>article</type><title>Antigens in perienteric fluid of Ascaris suum as detected through antibodies in pigs orally inoculated with fully embryonated eggs</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Rhodes, M.B. ; Staudinger, Lisa A.</creator><creatorcontrib>Rhodes, M.B. ; Staudinger, Lisa A.</creatorcontrib><description>Perienteric fluid (Pf) of adult
Ascaris suum was fractionated by ammonium sulfate precipitation, gel filtration or DEAE-cellulose chromatography, and sucrose density gradient centrifugation. Anti-sera (anti-EE) from pigs which were inoculated orally with fully embryonated eggs (EE) of
A. suum were used in an indirect radioimmunoassay (IRIA) to determine which fractions of Pf reacted positively in the analyses at the lowest protein concentration. These fractions were considered to contain more potent antigens. Comparative IRIA were performed employing antisera (anti-Pf) produced by injecting Pf into pigs. Six out of 35 fractions reacted positively at ⩽ 0.2
μg protein when anti-EE was used in the IRIA. Twenty-two out of 35 fractions reacted positively at ⩽ 0.2
μg protein when anti-Pf was used. Five of the 6 fractions reacting positively with anti-EE also reacted with anti-Pf. A 76 000 dalton component appears to be one of the major proteins in the 6 fractions which react positively with anti-EE while components from 10 000–138 000 daltons were present in the various fractions reacting positively with anti-Pf at ⩽ 0.2
μg protein.</description><identifier>ISSN: 0304-4017</identifier><identifier>EISSN: 1873-2550</identifier><identifier>DOI: 10.1016/0304-4017(83)90006-7</identifier><identifier>PMID: 6684353</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antibodies - immunology ; Antigens - analysis ; Ascariasis - immunology ; Ascariasis - veterinary ; Ascaris - immunology ; Ascaris suum ; Centrifugation, Density Gradient ; Chromatography, DEAE-Cellulose ; Electrophoresis, Polyacrylamide Gel ; Radioimmunoassay ; Sodium Dodecyl Sulfate ; Swine - immunology ; Swine Diseases - immunology</subject><ispartof>Veterinary parasitology, 1983-01, Vol.12 (2), p.179-186</ispartof><rights>1983</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-8e2271ae11715a80126f12f745525a18df341122c13ab7739515cd92566443f63</citedby><cites>FETCH-LOGICAL-c388t-8e2271ae11715a80126f12f745525a18df341122c13ab7739515cd92566443f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0304401783900067$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6684353$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rhodes, M.B.</creatorcontrib><creatorcontrib>Staudinger, Lisa A.</creatorcontrib><title>Antigens in perienteric fluid of Ascaris suum as detected through antibodies in pigs orally inoculated with fully embryonated eggs</title><title>Veterinary parasitology</title><addtitle>Vet Parasitol</addtitle><description>Perienteric fluid (Pf) of adult
Ascaris suum was fractionated by ammonium sulfate precipitation, gel filtration or DEAE-cellulose chromatography, and sucrose density gradient centrifugation. Anti-sera (anti-EE) from pigs which were inoculated orally with fully embryonated eggs (EE) of
A. suum were used in an indirect radioimmunoassay (IRIA) to determine which fractions of Pf reacted positively in the analyses at the lowest protein concentration. These fractions were considered to contain more potent antigens. Comparative IRIA were performed employing antisera (anti-Pf) produced by injecting Pf into pigs. Six out of 35 fractions reacted positively at ⩽ 0.2
μg protein when anti-EE was used in the IRIA. Twenty-two out of 35 fractions reacted positively at ⩽ 0.2
μg protein when anti-Pf was used. Five of the 6 fractions reacting positively with anti-EE also reacted with anti-Pf. A 76 000 dalton component appears to be one of the major proteins in the 6 fractions which react positively with anti-EE while components from 10 000–138 000 daltons were present in the various fractions reacting positively with anti-Pf at ⩽ 0.2
μg protein.</description><subject>Animals</subject><subject>Antibodies - immunology</subject><subject>Antigens - analysis</subject><subject>Ascariasis - immunology</subject><subject>Ascariasis - veterinary</subject><subject>Ascaris - immunology</subject><subject>Ascaris suum</subject><subject>Centrifugation, Density Gradient</subject><subject>Chromatography, DEAE-Cellulose</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Radioimmunoassay</subject><subject>Sodium Dodecyl Sulfate</subject><subject>Swine - immunology</subject><subject>Swine Diseases - immunology</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi1EVZbCPwDJJwSHFE_8mQvSquJLqtQLnC2vM8kaJfFiJ1R75ZfX6a56hIstzzzzWpqHkDfAroGB-sg4E5VgoN8b_qFhjKlKPyMbMJpXtZTsOdk8IS_Iy5x_FUYwpS_JpVJGcMk35O92mkOPU6ZhogdMAae5nJ52wxJaGju6zd6lkGlelpG6TFuc0c_Y0nmf4tLvqSsJu9gGPGWEPtOY3DAcyzP6ZXArfB_mPe2WtYrjLh3j9FjGvs-vyEXnhoyvz_cV-fnl84-bb9Xt3dfvN9vbynNj5spgXWtwCKBBOsOgVh3UnRZS1tKBaTsuAOraA3c7rXkjQfq2qaVSQvBO8Svy7pR7SPH3gnm2Y8geh8FNGJdsDVO8YU3zXxC4FkaDKaA4gT7FnBN29pDC6NLRArOrI7sKsKsAa7h9dGR1GXt7zl92I7ZPQ2cppf_p1MeyjT8Bk82-ePHYhlRWb9sY_v3BA7r-oMw</recordid><startdate>19830101</startdate><enddate>19830101</enddate><creator>Rhodes, M.B.</creator><creator>Staudinger, Lisa A.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19830101</creationdate><title>Antigens in perienteric fluid of Ascaris suum as detected through antibodies in pigs orally inoculated with fully embryonated eggs</title><author>Rhodes, M.B. ; Staudinger, Lisa A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-8e2271ae11715a80126f12f745525a18df341122c13ab7739515cd92566443f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Animals</topic><topic>Antibodies - immunology</topic><topic>Antigens - analysis</topic><topic>Ascariasis - immunology</topic><topic>Ascariasis - veterinary</topic><topic>Ascaris - immunology</topic><topic>Ascaris suum</topic><topic>Centrifugation, Density Gradient</topic><topic>Chromatography, DEAE-Cellulose</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Radioimmunoassay</topic><topic>Sodium Dodecyl Sulfate</topic><topic>Swine - immunology</topic><topic>Swine Diseases - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rhodes, M.B.</creatorcontrib><creatorcontrib>Staudinger, Lisa A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rhodes, M.B.</au><au>Staudinger, Lisa A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antigens in perienteric fluid of Ascaris suum as detected through antibodies in pigs orally inoculated with fully embryonated eggs</atitle><jtitle>Veterinary parasitology</jtitle><addtitle>Vet Parasitol</addtitle><date>1983-01-01</date><risdate>1983</risdate><volume>12</volume><issue>2</issue><spage>179</spage><epage>186</epage><pages>179-186</pages><issn>0304-4017</issn><eissn>1873-2550</eissn><abstract>Perienteric fluid (Pf) of adult
Ascaris suum was fractionated by ammonium sulfate precipitation, gel filtration or DEAE-cellulose chromatography, and sucrose density gradient centrifugation. Anti-sera (anti-EE) from pigs which were inoculated orally with fully embryonated eggs (EE) of
A. suum were used in an indirect radioimmunoassay (IRIA) to determine which fractions of Pf reacted positively in the analyses at the lowest protein concentration. These fractions were considered to contain more potent antigens. Comparative IRIA were performed employing antisera (anti-Pf) produced by injecting Pf into pigs. Six out of 35 fractions reacted positively at ⩽ 0.2
μg protein when anti-EE was used in the IRIA. Twenty-two out of 35 fractions reacted positively at ⩽ 0.2
μg protein when anti-Pf was used. Five of the 6 fractions reacting positively with anti-EE also reacted with anti-Pf. A 76 000 dalton component appears to be one of the major proteins in the 6 fractions which react positively with anti-EE while components from 10 000–138 000 daltons were present in the various fractions reacting positively with anti-Pf at ⩽ 0.2
μg protein.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>6684353</pmid><doi>10.1016/0304-4017(83)90006-7</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-4017 |
| ispartof | Veterinary parasitology, 1983-01, Vol.12 (2), p.179-186 |
| issn | 0304-4017 1873-2550 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80639099 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antibodies - immunology Antigens - analysis Ascariasis - immunology Ascariasis - veterinary Ascaris - immunology Ascaris suum Centrifugation, Density Gradient Chromatography, DEAE-Cellulose Electrophoresis, Polyacrylamide Gel Radioimmunoassay Sodium Dodecyl Sulfate Swine - immunology Swine Diseases - immunology |
| title | Antigens in perienteric fluid of Ascaris suum as detected through antibodies in pigs orally inoculated with fully embryonated eggs |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-19T22%3A23%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Antigens%20in%20perienteric%20fluid%20of%20Ascaris%20suum%20as%20detected%20through%20antibodies%20in%20pigs%20orally%20inoculated%20with%20fully%20embryonated%20eggs&rft.jtitle=Veterinary%20parasitology&rft.au=Rhodes,%20M.B.&rft.date=1983-01-01&rft.volume=12&rft.issue=2&rft.spage=179&rft.epage=186&rft.pages=179-186&rft.issn=0304-4017&rft.eissn=1873-2550&rft_id=info:doi/10.1016/0304-4017(83)90006-7&rft_dat=%3Cproquest_cross%3E80639099%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=13748718&rft_id=info:pmid/6684353&rft_els_id=0304401783900067&rfr_iscdi=true |