Properties of Colony Promoting Activity in Procine Kidney Extract
Aqueous extracts prepared from procine kidneys (PKE) possess colony-promoting activity (CPA) which increases the number of granulocyte and macrophage colonies in semi-solid cultures of mouse bone marrow cells (BMC) in the presence of colony-stimulating factor (GM-CSF).PKE was totally inactivated by...
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| Veröffentlicht in: | Chemical & pharmaceutical bulletin 1991/02/25, Vol.39(2), pp.425-427 |
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| creator | KASHIWAKURA, Ikuo HAYASE, Yukitoshi TAKAGI, Yoshinari |
| description | Aqueous extracts prepared from procine kidneys (PKE) possess colony-promoting activity (CPA) which increases the number of granulocyte and macrophage colonies in semi-solid cultures of mouse bone marrow cells (BMC) in the presence of colony-stimulating factor (GM-CSF).PKE was totally inactivated by 15 mM N-ethylmaleimide, but was resistant to 5 mM dithiothreitol (DTT), 50 mM sodium metaperiodate and a mixture of diisopropylether-n-BuOH (3 : 2).The proportion of deoxyribonucleic acid (DNA)-synthesizing cells of the PKE-responsive cells was about one-half in comparison to those of CSF-responsive cells, as estimated using the hydroxyurea (HU) suicide method.Upon marrow preincubation with PKE in liquid culture for 24 h, the suicide rate of the colony forming unit in culture (CFU-C) by HU increased to 3 times compared to that of the control.Since cyclophosphamide (CY) induces a change in the number of CFU-C, the effects of PKE on BMC obtained from CY injected mice were investigated. On day 1, the number of PKE-responsive cells significantly increased by about 2.3 times in comparison with that of control, whereas the number of CFU-C per 1×104 cells significantly decreased to about one-eighth of that of control.These results suggest that a sulfhydryl group(s) is required for the appearance of the colony-promoting activity of PKE, and glycoproteins, glycopeptides or hydrophobic components are not required; they also suggest that PKE may act on immature granulocyte/macrophage progenitors, which are youger than CSF-responsive CFU-C. |
| doi_str_mv | 10.1248/cpb.39.425 |
| format | Article |
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On day 1, the number of PKE-responsive cells significantly increased by about 2.3 times in comparison with that of control, whereas the number of CFU-C per 1×104 cells significantly decreased to about one-eighth of that of control.These results suggest that a sulfhydryl group(s) is required for the appearance of the colony-promoting activity of PKE, and glycoproteins, glycopeptides or hydrophobic components are not required; they also suggest that PKE may act on immature granulocyte/macrophage progenitors, which are youger than CSF-responsive CFU-C.</description><identifier>ISSN: 0009-2363</identifier><identifier>EISSN: 1347-5223</identifier><identifier>DOI: 10.1248/cpb.39.425</identifier><identifier>PMID: 2054867</identifier><identifier>CODEN: CPBTAL</identifier><language>eng</language><publisher>Tokyo: The Pharmaceutical Society of Japan</publisher><subject>Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; bone marrow culture ; CFU-C ; colony promoting activity ; Colony-Forming Units Assay ; Colony-Stimulating Factors - pharmacology ; Fundamental and applied biological sciences. Psychology ; Kidney - physiology ; kidney extract ; Male ; Mice ; Mice, Inbred Strains ; Protein hormones. Growth factors. Cytokines ; Proteins ; Swine ; Tissue Extracts - pharmacology</subject><ispartof>Chemical and Pharmaceutical Bulletin, 1991/02/25, Vol.39(2), pp.425-427</ispartof><rights>The Pharmaceutical Society of Japan</rights><rights>1992 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1991</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4951581$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2054867$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KASHIWAKURA, Ikuo</creatorcontrib><creatorcontrib>HAYASE, Yukitoshi</creatorcontrib><creatorcontrib>TAKAGI, Yoshinari</creatorcontrib><title>Properties of Colony Promoting Activity in Procine Kidney Extract</title><title>Chemical & pharmaceutical bulletin</title><addtitle>Chem. Pharm. Bull.</addtitle><description>Aqueous extracts prepared from procine kidneys (PKE) possess colony-promoting activity (CPA) which increases the number of granulocyte and macrophage colonies in semi-solid cultures of mouse bone marrow cells (BMC) in the presence of colony-stimulating factor (GM-CSF).PKE was totally inactivated by 15 mM N-ethylmaleimide, but was resistant to 5 mM dithiothreitol (DTT), 50 mM sodium metaperiodate and a mixture of diisopropylether-n-BuOH (3 : 2).The proportion of deoxyribonucleic acid (DNA)-synthesizing cells of the PKE-responsive cells was about one-half in comparison to those of CSF-responsive cells, as estimated using the hydroxyurea (HU) suicide method.Upon marrow preincubation with PKE in liquid culture for 24 h, the suicide rate of the colony forming unit in culture (CFU-C) by HU increased to 3 times compared to that of the control.Since cyclophosphamide (CY) induces a change in the number of CFU-C, the effects of PKE on BMC obtained from CY injected mice were investigated. On day 1, the number of PKE-responsive cells significantly increased by about 2.3 times in comparison with that of control, whereas the number of CFU-C per 1×104 cells significantly decreased to about one-eighth of that of control.These results suggest that a sulfhydryl group(s) is required for the appearance of the colony-promoting activity of PKE, and glycoproteins, glycopeptides or hydrophobic components are not required; they also suggest that PKE may act on immature granulocyte/macrophage progenitors, which are youger than CSF-responsive CFU-C.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>bone marrow culture</subject><subject>CFU-C</subject><subject>colony promoting activity</subject><subject>Colony-Forming Units Assay</subject><subject>Colony-Stimulating Factors - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Kidney - physiology</subject><subject>kidney extract</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Protein hormones. Growth factors. Cytokines</subject><subject>Proteins</subject><subject>Swine</subject><subject>Tissue Extracts - pharmacology</subject><issn>0009-2363</issn><issn>1347-5223</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkEtLAzEURoMoWqsb98KA4kKYmncyy1LqAwVd6Dpk0kxNmSY1mYr992ZoqeAmgfsd7nc5AFwgOEKYyjuzqkekGlHMDsAAESpKhjE5BAMIYVViwskJOE1pASFmUJBjcIwho5KLARi_xbCysXM2FaEpJqENflPk4TJ0zs-Lsenct-s2hfP91Dhvi2c383ZTTH-6qE13Bo4a3SZ7vvuH4ON--j55LF9eH54m45fSMMhYiSXkNaF1I2rNBRKUQKlnvGokr5HEpKKNtJIbyliDuSSIMmQFRoxgVgtqyRDcbPeuYvha29SppUvGtq32NqyTyvuJQAhl8OofuAjr6PNtClEOcfYjeup2S5kYUoq2UavoljpuFIKqt6qyVUUqla1m-HK3cl0v7WyP7jTm_HqX62R020TtjUt7jFYMMdl3TrbYInV6bve5zv5Na_tGVDHZt-Ltk8v_0k8dlfXkF9uDk8o</recordid><startdate>1991</startdate><enddate>1991</enddate><creator>KASHIWAKURA, Ikuo</creator><creator>HAYASE, Yukitoshi</creator><creator>TAKAGI, Yoshinari</creator><general>The Pharmaceutical Society of Japan</general><general>Maruzen</general><general>Japan Science and Technology Agency</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>1991</creationdate><title>Properties of Colony Promoting Activity in Procine Kidney Extract</title><author>KASHIWAKURA, Ikuo ; HAYASE, Yukitoshi ; TAKAGI, Yoshinari</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5055-2806b34bf7ba67174308ad69f86b182394f8e86c455f26831451e7215325b74e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>bone marrow culture</topic><topic>CFU-C</topic><topic>colony promoting activity</topic><topic>Colony-Forming Units Assay</topic><topic>Colony-Stimulating Factors - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Kidney - physiology</topic><topic>kidney extract</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Protein hormones. Growth factors. Cytokines</topic><topic>Proteins</topic><topic>Swine</topic><topic>Tissue Extracts - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KASHIWAKURA, Ikuo</creatorcontrib><creatorcontrib>HAYASE, Yukitoshi</creatorcontrib><creatorcontrib>TAKAGI, Yoshinari</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Chemical & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KASHIWAKURA, Ikuo</au><au>HAYASE, Yukitoshi</au><au>TAKAGI, Yoshinari</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Properties of Colony Promoting Activity in Procine Kidney Extract</atitle><jtitle>Chemical & pharmaceutical bulletin</jtitle><addtitle>Chem. Pharm. Bull.</addtitle><date>1991</date><risdate>1991</risdate><volume>39</volume><issue>2</issue><spage>425</spage><epage>427</epage><pages>425-427</pages><issn>0009-2363</issn><eissn>1347-5223</eissn><coden>CPBTAL</coden><abstract>Aqueous extracts prepared from procine kidneys (PKE) possess colony-promoting activity (CPA) which increases the number of granulocyte and macrophage colonies in semi-solid cultures of mouse bone marrow cells (BMC) in the presence of colony-stimulating factor (GM-CSF).PKE was totally inactivated by 15 mM N-ethylmaleimide, but was resistant to 5 mM dithiothreitol (DTT), 50 mM sodium metaperiodate and a mixture of diisopropylether-n-BuOH (3 : 2).The proportion of deoxyribonucleic acid (DNA)-synthesizing cells of the PKE-responsive cells was about one-half in comparison to those of CSF-responsive cells, as estimated using the hydroxyurea (HU) suicide method.Upon marrow preincubation with PKE in liquid culture for 24 h, the suicide rate of the colony forming unit in culture (CFU-C) by HU increased to 3 times compared to that of the control.Since cyclophosphamide (CY) induces a change in the number of CFU-C, the effects of PKE on BMC obtained from CY injected mice were investigated. On day 1, the number of PKE-responsive cells significantly increased by about 2.3 times in comparison with that of control, whereas the number of CFU-C per 1×104 cells significantly decreased to about one-eighth of that of control.These results suggest that a sulfhydryl group(s) is required for the appearance of the colony-promoting activity of PKE, and glycoproteins, glycopeptides or hydrophobic components are not required; they also suggest that PKE may act on immature granulocyte/macrophage progenitors, which are youger than CSF-responsive CFU-C.</abstract><cop>Tokyo</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>2054867</pmid><doi>10.1248/cpb.39.425</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Analytical, structural and metabolic biochemistry Animals Biological and medical sciences bone marrow culture CFU-C colony promoting activity Colony-Forming Units Assay Colony-Stimulating Factors - pharmacology Fundamental and applied biological sciences. Psychology Kidney - physiology kidney extract Male Mice Mice, Inbred Strains Protein hormones. Growth factors. Cytokines Proteins Swine Tissue Extracts - pharmacology |
| title | Properties of Colony Promoting Activity in Procine Kidney Extract |
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