Differential regulation of insect globin and actin mRNAs during larval development in Chironomus thummi
S1 nuclease protection assays were used to measure changes in the steady-state levels of six different globin ( Gb) mRNAs in the midge, Chironomus thummi thummi ( C. thummi, Diptera) during larval development. Two distinct patterns of change were observed. GbI, IV, VIIB-4 and VIIB-5 transcripts were...
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| Veröffentlicht in: | Gene 1991-05, Vol.101 (2), p.215-222 |
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| creator | Saffarini, Daad A. Trewitt, Patrick M. Luhm, Robert A. Bergtrom, Gerald |
| description | S1 nuclease protection assays were used to measure changes in the steady-state levels of six different globin (
Gb) mRNAs in the midge,
Chironomus thummi thummi (
C. thummi, Diptera) during larval development. Two distinct patterns of change were observed.
GbI,
IV,
VIIB-4 and
VIIB-5 transcripts were present in 3rd instar larvae, rose from low levels immediately post-moult to peak levels by day 2–3 of the 4th instar, and then declined, reaching near-basal levels by day 7–8. In contrast, transcripts of
GbIII (known from previous studies to be 4th instar-specific) and
VI, which were undetectable in the 3rd instar, rose to high levels by day 2 of the 4th instar, but remained elevated thereafter. Our data further showed that closely linked
Gb genes were not necessarily expressed in a coordinate manner. Unlike the
Gb mRNAs, actin (
Act) mRNA levels (measured by slot-blot hybridization to a heterologous probe) increased progressively as a proportion of total RNA during 4th instar development. Therefore, the regulation of
C. thummi Gb transcript levels is specific, differing from that of
Act and among the
Gb mRNAs themselves. Elevated 20-hydroxyecdysone (HE) titer at the 3rd-4th instar moult correlates with the low steady-state levels of
Gb mRNAs immediately post-moult. However, other aspects of
Gb mRNA profiles cannot be explained on the basis of a direct repressive effect by HE on
Gb gene transcription. |
| doi_str_mv | 10.1016/0378-1119(91)90414-7 |
| format | Article |
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Gb) mRNAs in the midge,
Chironomus thummi thummi (
C. thummi, Diptera) during larval development. Two distinct patterns of change were observed.
GbI,
IV,
VIIB-4 and
VIIB-5 transcripts were present in 3rd instar larvae, rose from low levels immediately post-moult to peak levels by day 2–3 of the 4th instar, and then declined, reaching near-basal levels by day 7–8. In contrast, transcripts of
GbIII (known from previous studies to be 4th instar-specific) and
VI, which were undetectable in the 3rd instar, rose to high levels by day 2 of the 4th instar, but remained elevated thereafter. Our data further showed that closely linked
Gb genes were not necessarily expressed in a coordinate manner. Unlike the
Gb mRNAs, actin (
Act) mRNA levels (measured by slot-blot hybridization to a heterologous probe) increased progressively as a proportion of total RNA during 4th instar development. Therefore, the regulation of
C. thummi Gb transcript levels is specific, differing from that of
Act and among the
Gb mRNAs themselves. Elevated 20-hydroxyecdysone (HE) titer at the 3rd-4th instar moult correlates with the low steady-state levels of
Gb mRNAs immediately post-moult. However, other aspects of
Gb mRNA profiles cannot be explained on the basis of a direct repressive effect by HE on
Gb gene transcription.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(91)90414-7</identifier><identifier>PMID: 2055487</identifier><identifier>CODEN: GENED6</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>20-hydroxyecdysone ; act gene ; actin ; Actins - genetics ; Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; biological development ; Chironomidae ; Chironomidae - drug effects ; Chironomidae - genetics ; Chironomidae - growth & development ; Chironomidae - metabolism ; Chironomus riparius ; Chironomus thummi thummi ; Diptera ; DNA, Recombinant ; ecdysterone ; Ecdysterone - pharmacology ; Fundamental and applied biological sciences. Psychology ; gb gene ; genbank/m57410 ; genbank/m57411 ; Gene expression ; Gene Expression Regulation ; genetic regulation ; globin mRNA accumulation ; globins ; Globins - genetics ; Larva - metabolism ; larvae ; messenger RNA ; midge ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; multigene family ; Recombinant DNA ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; S1 nuclease protection ; Single-Strand Specific DNA and RNA Endonucleases - metabolism</subject><ispartof>Gene, 1991-05, Vol.101 (2), p.215-222</ispartof><rights>1991</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-347e091081b236998c45ddede7a5747796d7dd6f0ce0f3fba87ec07d16ceae833</citedby><cites>FETCH-LOGICAL-c357t-347e091081b236998c45ddede7a5747796d7dd6f0ce0f3fba87ec07d16ceae833</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0378111991904147$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19749714$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2055487$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saffarini, Daad A.</creatorcontrib><creatorcontrib>Trewitt, Patrick M.</creatorcontrib><creatorcontrib>Luhm, Robert A.</creatorcontrib><creatorcontrib>Bergtrom, Gerald</creatorcontrib><title>Differential regulation of insect globin and actin mRNAs during larval development in Chironomus thummi</title><title>Gene</title><addtitle>Gene</addtitle><description>S1 nuclease protection assays were used to measure changes in the steady-state levels of six different globin (
Gb) mRNAs in the midge,
Chironomus thummi thummi (
C. thummi, Diptera) during larval development. Two distinct patterns of change were observed.
GbI,
IV,
VIIB-4 and
VIIB-5 transcripts were present in 3rd instar larvae, rose from low levels immediately post-moult to peak levels by day 2–3 of the 4th instar, and then declined, reaching near-basal levels by day 7–8. In contrast, transcripts of
GbIII (known from previous studies to be 4th instar-specific) and
VI, which were undetectable in the 3rd instar, rose to high levels by day 2 of the 4th instar, but remained elevated thereafter. Our data further showed that closely linked
Gb genes were not necessarily expressed in a coordinate manner. Unlike the
Gb mRNAs, actin (
Act) mRNA levels (measured by slot-blot hybridization to a heterologous probe) increased progressively as a proportion of total RNA during 4th instar development. Therefore, the regulation of
C. thummi Gb transcript levels is specific, differing from that of
Act and among the
Gb mRNAs themselves. Elevated 20-hydroxyecdysone (HE) titer at the 3rd-4th instar moult correlates with the low steady-state levels of
Gb mRNAs immediately post-moult. However, other aspects of
Gb mRNA profiles cannot be explained on the basis of a direct repressive effect by HE on
Gb gene transcription.</description><subject>20-hydroxyecdysone</subject><subject>act gene</subject><subject>actin</subject><subject>Actins - genetics</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>biological development</subject><subject>Chironomidae</subject><subject>Chironomidae - drug effects</subject><subject>Chironomidae - genetics</subject><subject>Chironomidae - growth & development</subject><subject>Chironomidae - metabolism</subject><subject>Chironomus riparius</subject><subject>Chironomus thummi thummi</subject><subject>Diptera</subject><subject>DNA, Recombinant</subject><subject>ecdysterone</subject><subject>Ecdysterone - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gb gene</subject><subject>genbank/m57410</subject><subject>genbank/m57411</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>genetic regulation</subject><subject>globin mRNA accumulation</subject><subject>globins</subject><subject>Globins - genetics</subject><subject>Larva - metabolism</subject><subject>larvae</subject><subject>messenger RNA</subject><subject>midge</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>multigene family</subject><subject>Recombinant DNA</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>S1 nuclease protection</subject><subject>Single-Strand Specific DNA and RNA Endonucleases - metabolism</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuLFDEUhYMoYzv6DxSzUXRRmlSq8tgIQ8_4gEFBnXVIJzc1kaqkTaoa_Pem7GbcaTY3cL9zuJyD0FNK3lBC-VvChGwopeqVoq8V6WjXiHtoQ6VQDSFM3kebO-QhelTKD1Jf37dn6Kyts5Nig4bL4D1kiHMwI84wLKOZQ4o4eRxiATvjYUy7ELGJDhs719_09fNFwW7JIQ54NPlQlQ4OMKb9VI2qDm9vQ04xTUvB8-0yTeExeuDNWODJaZ6jm_dX37cfm-svHz5tL64by3oxN6wTQBQlku5axpWStuudAwfC9KITQnEnnOOeWCCe-Z2RAiwRjnILBiRj5-jl0Xef088FyqynUCyMo4mQlqIl4Yxz9X-Q8p6LvhUV7I6gzamUDF7vc5hM_qUp0WsRek1ZrylrRfWfIvQqe3byX3YTuDvRKfm6f3Ham2LN6LOJNpS_3kp0StCucs-PnDdJmyFX5uZbSygjVDAu1er07khAzfUQIOtiA0QLLuTan3Yp_PvU35A4rpo</recordid><startdate>19910530</startdate><enddate>19910530</enddate><creator>Saffarini, Daad A.</creator><creator>Trewitt, Patrick M.</creator><creator>Luhm, Robert A.</creator><creator>Bergtrom, Gerald</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19910530</creationdate><title>Differential regulation of insect globin and actin mRNAs during larval development in Chironomus thummi</title><author>Saffarini, Daad A. ; Trewitt, Patrick M. ; Luhm, Robert A. ; Bergtrom, Gerald</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-347e091081b236998c45ddede7a5747796d7dd6f0ce0f3fba87ec07d16ceae833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>20-hydroxyecdysone</topic><topic>act gene</topic><topic>actin</topic><topic>Actins - genetics</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>biological development</topic><topic>Chironomidae</topic><topic>Chironomidae - drug effects</topic><topic>Chironomidae - genetics</topic><topic>Chironomidae - growth & development</topic><topic>Chironomidae - metabolism</topic><topic>Chironomus riparius</topic><topic>Chironomus thummi thummi</topic><topic>Diptera</topic><topic>DNA, Recombinant</topic><topic>ecdysterone</topic><topic>Ecdysterone - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gb gene</topic><topic>genbank/m57410</topic><topic>genbank/m57411</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>genetic regulation</topic><topic>globin mRNA accumulation</topic><topic>globins</topic><topic>Globins - genetics</topic><topic>Larva - metabolism</topic><topic>larvae</topic><topic>messenger RNA</topic><topic>midge</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>multigene family</topic><topic>Recombinant DNA</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>S1 nuclease protection</topic><topic>Single-Strand Specific DNA and RNA Endonucleases - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saffarini, Daad A.</creatorcontrib><creatorcontrib>Trewitt, Patrick M.</creatorcontrib><creatorcontrib>Luhm, Robert A.</creatorcontrib><creatorcontrib>Bergtrom, Gerald</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saffarini, Daad A.</au><au>Trewitt, Patrick M.</au><au>Luhm, Robert A.</au><au>Bergtrom, Gerald</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential regulation of insect globin and actin mRNAs during larval development in Chironomus thummi</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1991-05-30</date><risdate>1991</risdate><volume>101</volume><issue>2</issue><spage>215</spage><epage>222</epage><pages>215-222</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><coden>GENED6</coden><abstract>S1 nuclease protection assays were used to measure changes in the steady-state levels of six different globin (
Gb) mRNAs in the midge,
Chironomus thummi thummi (
C. thummi, Diptera) during larval development. Two distinct patterns of change were observed.
GbI,
IV,
VIIB-4 and
VIIB-5 transcripts were present in 3rd instar larvae, rose from low levels immediately post-moult to peak levels by day 2–3 of the 4th instar, and then declined, reaching near-basal levels by day 7–8. In contrast, transcripts of
GbIII (known from previous studies to be 4th instar-specific) and
VI, which were undetectable in the 3rd instar, rose to high levels by day 2 of the 4th instar, but remained elevated thereafter. Our data further showed that closely linked
Gb genes were not necessarily expressed in a coordinate manner. Unlike the
Gb mRNAs, actin (
Act) mRNA levels (measured by slot-blot hybridization to a heterologous probe) increased progressively as a proportion of total RNA during 4th instar development. Therefore, the regulation of
C. thummi Gb transcript levels is specific, differing from that of
Act and among the
Gb mRNAs themselves. Elevated 20-hydroxyecdysone (HE) titer at the 3rd-4th instar moult correlates with the low steady-state levels of
Gb mRNAs immediately post-moult. However, other aspects of
Gb mRNA profiles cannot be explained on the basis of a direct repressive effect by HE on
Gb gene transcription.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>2055487</pmid><doi>10.1016/0378-1119(91)90414-7</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Gene, 1991-05, Vol.101 (2), p.215-222 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80636693 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 20-hydroxyecdysone act gene actin Actins - genetics Amino Acid Sequence Animals Base Sequence Biological and medical sciences biological development Chironomidae Chironomidae - drug effects Chironomidae - genetics Chironomidae - growth & development Chironomidae - metabolism Chironomus riparius Chironomus thummi thummi Diptera DNA, Recombinant ecdysterone Ecdysterone - pharmacology Fundamental and applied biological sciences. Psychology gb gene genbank/m57410 genbank/m57411 Gene expression Gene Expression Regulation genetic regulation globin mRNA accumulation globins Globins - genetics Larva - metabolism larvae messenger RNA midge Molecular and cellular biology Molecular genetics Molecular Sequence Data multigene family Recombinant DNA RNA, Messenger - genetics RNA, Messenger - metabolism S1 nuclease protection Single-Strand Specific DNA and RNA Endonucleases - metabolism |
| title | Differential regulation of insect globin and actin mRNAs during larval development in Chironomus thummi |
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