An over-expression plasmid for Escherichia coli primase

To facilitate the overexpression of Escherichia coli primase, the dnaG gene has been reconstructed using polymerase chain reaction to remove the 5′ transcription terminator and the 3′ RNA processing site. This construct was cloned into the T7 polymerase-transcribed expression vector, pET-3d. Cells c...

Ausführliche Beschreibung