A family of repeated DNA sequences in Toxoplasma gondii: Cloning, sequence analysis, and use in strain characterization
A Toxoplasma gondii genomic library was constructed in λEMBL3. Repeated fragments were detected by hybridization with radiolabeled total DNA from the parasite and one recombinant was chosen due to its strong hybridization signal. By using electrophoretic and hybridization analysis, four cross-hybrid...
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| Veröffentlicht in: | Experimental parasitology 1991-07, Vol.73 (1), p.73-81 |
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| creator | Cristina, Nadine Liaud, Marie-Françoise Santoro, Ferrucio Oury, Bruno Ambroise-Thomas, Pierre |
| description | A
Toxoplasma gondii genomic library was constructed in λEMBL3. Repeated fragments were detected by hybridization with radiolabeled total DNA from the parasite and one recombinant was chosen due to its strong hybridization signal. By using electrophoretic and hybridization analysis, four cross-hybridizating restriction fragments were selected and sequenced. The determined nucleotide sequence of these fragments (TGR1
A, TGR1
E, TGR2, and TGR4) has shown a complex system of conserved and degenerated repeats in which TGR1
E corresponds to the most conserved element. This last sequence was used to investigate restriction fragment length polymorphisms among several
T. gondii strains by Southern blotting. |
| doi_str_mv | 10.1016/0014-4894(91)90009-L |
| format | Article |
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Toxoplasma gondii genomic library was constructed in λEMBL3. Repeated fragments were detected by hybridization with radiolabeled total DNA from the parasite and one recombinant was chosen due to its strong hybridization signal. By using electrophoretic and hybridization analysis, four cross-hybridizating restriction fragments were selected and sequenced. The determined nucleotide sequence of these fragments (TGR1
A, TGR1
E, TGR2, and TGR4) has shown a complex system of conserved and degenerated repeats in which TGR1
E corresponds to the most conserved element. This last sequence was used to investigate restriction fragment length polymorphisms among several
T. gondii strains by Southern blotting.</description><identifier>ISSN: 0014-4894</identifier><identifier>EISSN: 1090-2449</identifier><identifier>DOI: 10.1016/0014-4894(91)90009-L</identifier><identifier>PMID: 1676001</identifier><identifier>CODEN: EXPAAA</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>ADN ; ADN RECOMBINADO ; ADN RECOMBINE ; Animals ; Base Sequence ; Biological and medical sciences ; Blotting, Southern ; CLONACION ; CLONAGE ; CLONING ; DNA ; DNA HYBRIDIZATION ; DNA LIBRARIES ; DNA, Protozoan - genetics ; Fundamental and applied biological sciences. Psychology ; GENBANK/M59716 ; GENBANK/M59717 ; GENBANK/M59718 ; GENBANK/M59719 ; GENETIC VARIATION ; Genomic Library ; MICE ; MOLECULAR SEQUENCE DATA ; NUCLEOTIDE ; NUCLEOTIDES ; NUCLEOTIDOS ; PATHOGENICITY ; PODER PATOGENO ; POLIMORFISMO ; POLYMORPHISM ; Polymorphism, Restriction Fragment Length ; POLYMORPHISME ; POUVOIR PATHOGENE ; Protozoa ; Protozoan, coccidian ; RATON ; RECOMBINANT DNA ; Repeated DNA sequence ; Repetitive Sequences, Nucleic Acid ; RESTRICTION FRAGMENT LENGTH POLYMORPHISM ; Restriction Mapping ; Sequence Homology, Nucleic Acid ; SOURIS ; Strains, characterization ; Systematics. Geographical distribution. Morphology. Cytology ; TOXOPLASMA ; Toxoplasma - classification ; Toxoplasma - genetics ; Toxoplasma gondii ; VARIACION GENETICA ; VARIATION GENETIQUE</subject><ispartof>Experimental parasitology, 1991-07, Vol.73 (1), p.73-81</ispartof><rights>1991</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-72e6bc9f15dc2a1e2e503737533f9aae1b8b38c53248ed650799a48b465c7eed3</citedby><cites>FETCH-LOGICAL-c405t-72e6bc9f15dc2a1e2e503737533f9aae1b8b38c53248ed650799a48b465c7eed3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/001448949190009L$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5141237$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1676001$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cristina, Nadine</creatorcontrib><creatorcontrib>Liaud, Marie-Françoise</creatorcontrib><creatorcontrib>Santoro, Ferrucio</creatorcontrib><creatorcontrib>Oury, Bruno</creatorcontrib><creatorcontrib>Ambroise-Thomas, Pierre</creatorcontrib><title>A family of repeated DNA sequences in Toxoplasma gondii: Cloning, sequence analysis, and use in strain characterization</title><title>Experimental parasitology</title><addtitle>Exp Parasitol</addtitle><description>A
Toxoplasma gondii genomic library was constructed in λEMBL3. Repeated fragments were detected by hybridization with radiolabeled total DNA from the parasite and one recombinant was chosen due to its strong hybridization signal. By using electrophoretic and hybridization analysis, four cross-hybridizating restriction fragments were selected and sequenced. The determined nucleotide sequence of these fragments (TGR1
A, TGR1
E, TGR2, and TGR4) has shown a complex system of conserved and degenerated repeats in which TGR1
E corresponds to the most conserved element. This last sequence was used to investigate restriction fragment length polymorphisms among several
T. gondii strains by Southern blotting.</description><subject>ADN</subject><subject>ADN RECOMBINADO</subject><subject>ADN RECOMBINE</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>CLONACION</subject><subject>CLONAGE</subject><subject>CLONING</subject><subject>DNA</subject><subject>DNA HYBRIDIZATION</subject><subject>DNA LIBRARIES</subject><subject>DNA, Protozoan - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENBANK/M59716</subject><subject>GENBANK/M59717</subject><subject>GENBANK/M59718</subject><subject>GENBANK/M59719</subject><subject>GENETIC VARIATION</subject><subject>Genomic Library</subject><subject>MICE</subject><subject>MOLECULAR SEQUENCE DATA</subject><subject>NUCLEOTIDE</subject><subject>NUCLEOTIDES</subject><subject>NUCLEOTIDOS</subject><subject>PATHOGENICITY</subject><subject>PODER PATOGENO</subject><subject>POLIMORFISMO</subject><subject>POLYMORPHISM</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>POLYMORPHISME</subject><subject>POUVOIR PATHOGENE</subject><subject>Protozoa</subject><subject>Protozoan, coccidian</subject><subject>RATON</subject><subject>RECOMBINANT DNA</subject><subject>Repeated DNA sequence</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>RESTRICTION FRAGMENT LENGTH POLYMORPHISM</subject><subject>Restriction Mapping</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>SOURIS</subject><subject>Strains, characterization</subject><subject>Systematics. Geographical distribution. Morphology. Cytology</subject><subject>TOXOPLASMA</subject><subject>Toxoplasma - classification</subject><subject>Toxoplasma - genetics</subject><subject>Toxoplasma gondii</subject><subject>VARIACION GENETICA</subject><subject>VARIATION GENETIQUE</subject><issn>0014-4894</issn><issn>1090-2449</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE2P0zAQhi3EaukW_gACyQe0YqUN2LGT2ByQqrJ8SBUc2D1bE2dSjJK42ClL-fU4pCo3TmPpfd7R-CHkGWevOOPla8a4zKTS8qXmV5oxprPNA7LgTLMsl1I_JIsT8ohcxPg9MYrn8pyc87IqU7gg9yvaQu-6A_UtDbhDGLGh7z6vaMQfexwsRuoGeut_-V0HsQe69UPj3Bu67vzghu31CaQwQHeILl6nV0P3EadmHAOkYb9BADticL9hdH54TM5a6CI-Oc4luXt_c7v-mG2-fPi0Xm0yK1kxZlWOZW11y4vG5sAxx4KJSlSFEK0GQF6rWihbiFwqbMqCVVqDVLUsC1shNmJJLue9u-DTmXE0vYsWuw4G9PtoFCuFUIVMoJxBG3yMAVuzC66HcDCcmcm3mWSaSabR3Pz1bTap9vy4f1_32PwrzYJT_uKYQ7TQtQEG6-IJK7jkefrQkjydsRa8gW1IyN1XzYUslU7h2znEJOqnw2CidZPxxgW0o2m8-_-RfwDQZaSq</recordid><startdate>19910701</startdate><enddate>19910701</enddate><creator>Cristina, Nadine</creator><creator>Liaud, Marie-Françoise</creator><creator>Santoro, Ferrucio</creator><creator>Oury, Bruno</creator><creator>Ambroise-Thomas, Pierre</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19910701</creationdate><title>A family of repeated DNA sequences in Toxoplasma gondii: Cloning, sequence analysis, and use in strain characterization</title><author>Cristina, Nadine ; Liaud, Marie-Françoise ; Santoro, Ferrucio ; Oury, Bruno ; Ambroise-Thomas, Pierre</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-72e6bc9f15dc2a1e2e503737533f9aae1b8b38c53248ed650799a48b465c7eed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>ADN</topic><topic>ADN RECOMBINADO</topic><topic>ADN RECOMBINE</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>CLONACION</topic><topic>CLONAGE</topic><topic>CLONING</topic><topic>DNA</topic><topic>DNA HYBRIDIZATION</topic><topic>DNA LIBRARIES</topic><topic>DNA, Protozoan - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENBANK/M59716</topic><topic>GENBANK/M59717</topic><topic>GENBANK/M59718</topic><topic>GENBANK/M59719</topic><topic>GENETIC VARIATION</topic><topic>Genomic Library</topic><topic>MICE</topic><topic>MOLECULAR SEQUENCE DATA</topic><topic>NUCLEOTIDE</topic><topic>NUCLEOTIDES</topic><topic>NUCLEOTIDOS</topic><topic>PATHOGENICITY</topic><topic>PODER PATOGENO</topic><topic>POLIMORFISMO</topic><topic>POLYMORPHISM</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>POLYMORPHISME</topic><topic>POUVOIR PATHOGENE</topic><topic>Protozoa</topic><topic>Protozoan, coccidian</topic><topic>RATON</topic><topic>RECOMBINANT DNA</topic><topic>Repeated DNA sequence</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>RESTRICTION FRAGMENT LENGTH POLYMORPHISM</topic><topic>Restriction Mapping</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>SOURIS</topic><topic>Strains, characterization</topic><topic>Systematics. Geographical distribution. Morphology. Cytology</topic><topic>TOXOPLASMA</topic><topic>Toxoplasma - classification</topic><topic>Toxoplasma - genetics</topic><topic>Toxoplasma gondii</topic><topic>VARIACION GENETICA</topic><topic>VARIATION GENETIQUE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cristina, Nadine</creatorcontrib><creatorcontrib>Liaud, Marie-Françoise</creatorcontrib><creatorcontrib>Santoro, Ferrucio</creatorcontrib><creatorcontrib>Oury, Bruno</creatorcontrib><creatorcontrib>Ambroise-Thomas, Pierre</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cristina, Nadine</au><au>Liaud, Marie-Françoise</au><au>Santoro, Ferrucio</au><au>Oury, Bruno</au><au>Ambroise-Thomas, Pierre</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A family of repeated DNA sequences in Toxoplasma gondii: Cloning, sequence analysis, and use in strain characterization</atitle><jtitle>Experimental parasitology</jtitle><addtitle>Exp Parasitol</addtitle><date>1991-07-01</date><risdate>1991</risdate><volume>73</volume><issue>1</issue><spage>73</spage><epage>81</epage><pages>73-81</pages><issn>0014-4894</issn><eissn>1090-2449</eissn><coden>EXPAAA</coden><abstract>A
Toxoplasma gondii genomic library was constructed in λEMBL3. Repeated fragments were detected by hybridization with radiolabeled total DNA from the parasite and one recombinant was chosen due to its strong hybridization signal. By using electrophoretic and hybridization analysis, four cross-hybridizating restriction fragments were selected and sequenced. The determined nucleotide sequence of these fragments (TGR1
A, TGR1
E, TGR2, and TGR4) has shown a complex system of conserved and degenerated repeats in which TGR1
E corresponds to the most conserved element. This last sequence was used to investigate restriction fragment length polymorphisms among several
T. gondii strains by Southern blotting.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1676001</pmid><doi>10.1016/0014-4894(91)90009-L</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-4894 |
| ispartof | Experimental parasitology, 1991-07, Vol.73 (1), p.73-81 |
| issn | 0014-4894 1090-2449 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80633854 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | ADN ADN RECOMBINADO ADN RECOMBINE Animals Base Sequence Biological and medical sciences Blotting, Southern CLONACION CLONAGE CLONING DNA DNA HYBRIDIZATION DNA LIBRARIES DNA, Protozoan - genetics Fundamental and applied biological sciences. Psychology GENBANK/M59716 GENBANK/M59717 GENBANK/M59718 GENBANK/M59719 GENETIC VARIATION Genomic Library MICE MOLECULAR SEQUENCE DATA NUCLEOTIDE NUCLEOTIDES NUCLEOTIDOS PATHOGENICITY PODER PATOGENO POLIMORFISMO POLYMORPHISM Polymorphism, Restriction Fragment Length POLYMORPHISME POUVOIR PATHOGENE Protozoa Protozoan, coccidian RATON RECOMBINANT DNA Repeated DNA sequence Repetitive Sequences, Nucleic Acid RESTRICTION FRAGMENT LENGTH POLYMORPHISM Restriction Mapping Sequence Homology, Nucleic Acid SOURIS Strains, characterization Systematics. Geographical distribution. Morphology. Cytology TOXOPLASMA Toxoplasma - classification Toxoplasma - genetics Toxoplasma gondii VARIACION GENETICA VARIATION GENETIQUE |
| title | A family of repeated DNA sequences in Toxoplasma gondii: Cloning, sequence analysis, and use in strain characterization |
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