Preparation and evaluation of steroid chemiluminescent tracers
Six different isoluminol derivatives have been synthesized and evaluated as potential labels for conjugation to steroid molecules. The resulting steroid-chemiluminescent tracers have been investigated in terms of detection limit and affinity for the homologous antibody. The conjugation to the steroi...
Gespeichert in:
Veröffentlicht in: | Journal of steroid biochemistry 1983-07, Vol.19 (1), p.407-412 |
---|---|
Hauptverfasser: | , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 412 |
---|---|
container_issue | 1 |
container_start_page | 407 |
container_title | Journal of steroid biochemistry |
container_volume | 19 |
creator | Pazzagli, M. Messeri, G. Caldini, A.L. Moneti, G. Martinazzo, G. Serio, M. |
description | Six different isoluminol derivatives have been synthesized and evaluated as potential labels for conjugation to steroid molecules. The resulting steroid-chemiluminescent tracers have been investigated in terms of detection limit and affinity for the homologous antibody. The conjugation to the steroid molecule did not significantly affect the quantum yield of the chemiluminescent label. Consequently the detection limit was very similar using tracers with the same chemiluminescent label and different steroids and it was always at picomolar levels. The affinity of the chemiluminescent tracer versus the homologous antibody was evaluated using tritiated steroids as reference. The bridge and the chemiluminescent molecule could influence the affinity of the chemiluminescent tracer which could be higher, similar or lower than that of the tritiated steroid. In conclusion some isoluminol derivatives (i.e. ABEI or AEEI) can be considered as universal labelling compounds for steroids and their metabolites, because they possess a high light efficiency (similar to luminal itself) and favourable reaction conditions. Moreover the conjugation to the steroid molecule does not modify their chemiluminescent properties. |
doi_str_mv | 10.1016/0022-4731(83)90195-4 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_80630248</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0022473183901954</els_id><sourcerecordid>80630248</sourcerecordid><originalsourceid>FETCH-LOGICAL-c338t-8b660a7ef8805481f68dfcf64e0735cde93fa82d7385e9f7fcf82c240c6e7f993</originalsourceid><addsrcrecordid>eNp9kEtLxDAQx3NQ1nX1Gyj0JHqopk2aTi8LsviCBT3oOWSTCUb6WJN2wW9vapc9yhyGmf88f4RcZPQ2o5m4ozTPU16y7BrYTUWzqkj5EZkf0ifkNIQvGgXg-YzMBEAZbU6Wbx63yqvedW2iWpPgTtXDFHY2CT36zplEf2Lj6qFxLQaNbZ_0Xmn04YwcW1UHPN_7Bfl4fHhfPafr16eX1f061YxBn8JGCKpKtAC04JBZAcZqKzjSkhXaYMWsgtyUDAqsbBk1yHXOqRZY2qpiC3I1zd367nvA0MvGxUPqWrXYDUECFYzmHGIhnwq170LwaOXWu0b5H5lROaKSIxM5MpHA5B8qyWPb5X7-sGnQHJr2nKK-nHSMT-4cehm0w1ajcR51L03n_l_wC7YxesM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>80630248</pqid></control><display><type>article</type><title>Preparation and evaluation of steroid chemiluminescent tracers</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Pazzagli, M. ; Messeri, G. ; Caldini, A.L. ; Moneti, G. ; Martinazzo, G. ; Serio, M.</creator><creatorcontrib>Pazzagli, M. ; Messeri, G. ; Caldini, A.L. ; Moneti, G. ; Martinazzo, G. ; Serio, M.</creatorcontrib><description>Six different isoluminol derivatives have been synthesized and evaluated as potential labels for conjugation to steroid molecules. The resulting steroid-chemiluminescent tracers have been investigated in terms of detection limit and affinity for the homologous antibody. The conjugation to the steroid molecule did not significantly affect the quantum yield of the chemiluminescent label. Consequently the detection limit was very similar using tracers with the same chemiluminescent label and different steroids and it was always at picomolar levels. The affinity of the chemiluminescent tracer versus the homologous antibody was evaluated using tritiated steroids as reference. The bridge and the chemiluminescent molecule could influence the affinity of the chemiluminescent tracer which could be higher, similar or lower than that of the tritiated steroid. In conclusion some isoluminol derivatives (i.e. ABEI or AEEI) can be considered as universal labelling compounds for steroids and their metabolites, because they possess a high light efficiency (similar to luminal itself) and favourable reaction conditions. Moreover the conjugation to the steroid molecule does not modify their chemiluminescent properties.</description><identifier>ISSN: 0022-4731</identifier><identifier>DOI: 10.1016/0022-4731(83)90195-4</identifier><identifier>PMID: 6887878</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Animals ; Cross Reactions ; Hormones - analysis ; Humans ; Hydrogen Peroxide ; Hydrogen-Ion Concentration ; Immunoassay ; Indicators and Reagents ; Luminescent Measurements ; Mass Spectrometry - methods ; Peroxidases ; Steroids - analysis ; Structure-Activity Relationship</subject><ispartof>Journal of steroid biochemistry, 1983-07, Vol.19 (1), p.407-412</ispartof><rights>1983</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c338t-8b660a7ef8805481f68dfcf64e0735cde93fa82d7385e9f7fcf82c240c6e7f993</citedby><cites>FETCH-LOGICAL-c338t-8b660a7ef8805481f68dfcf64e0735cde93fa82d7385e9f7fcf82c240c6e7f993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6887878$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pazzagli, M.</creatorcontrib><creatorcontrib>Messeri, G.</creatorcontrib><creatorcontrib>Caldini, A.L.</creatorcontrib><creatorcontrib>Moneti, G.</creatorcontrib><creatorcontrib>Martinazzo, G.</creatorcontrib><creatorcontrib>Serio, M.</creatorcontrib><title>Preparation and evaluation of steroid chemiluminescent tracers</title><title>Journal of steroid biochemistry</title><addtitle>J Steroid Biochem</addtitle><description>Six different isoluminol derivatives have been synthesized and evaluated as potential labels for conjugation to steroid molecules. The resulting steroid-chemiluminescent tracers have been investigated in terms of detection limit and affinity for the homologous antibody. The conjugation to the steroid molecule did not significantly affect the quantum yield of the chemiluminescent label. Consequently the detection limit was very similar using tracers with the same chemiluminescent label and different steroids and it was always at picomolar levels. The affinity of the chemiluminescent tracer versus the homologous antibody was evaluated using tritiated steroids as reference. The bridge and the chemiluminescent molecule could influence the affinity of the chemiluminescent tracer which could be higher, similar or lower than that of the tritiated steroid. In conclusion some isoluminol derivatives (i.e. ABEI or AEEI) can be considered as universal labelling compounds for steroids and their metabolites, because they possess a high light efficiency (similar to luminal itself) and favourable reaction conditions. Moreover the conjugation to the steroid molecule does not modify their chemiluminescent properties.</description><subject>Animals</subject><subject>Cross Reactions</subject><subject>Hormones - analysis</subject><subject>Humans</subject><subject>Hydrogen Peroxide</subject><subject>Hydrogen-Ion Concentration</subject><subject>Immunoassay</subject><subject>Indicators and Reagents</subject><subject>Luminescent Measurements</subject><subject>Mass Spectrometry - methods</subject><subject>Peroxidases</subject><subject>Steroids - analysis</subject><subject>Structure-Activity Relationship</subject><issn>0022-4731</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLxDAQx3NQ1nX1Gyj0JHqopk2aTi8LsviCBT3oOWSTCUb6WJN2wW9vapc9yhyGmf88f4RcZPQ2o5m4ozTPU16y7BrYTUWzqkj5EZkf0ifkNIQvGgXg-YzMBEAZbU6Wbx63yqvedW2iWpPgTtXDFHY2CT36zplEf2Lj6qFxLQaNbZ_0Xmn04YwcW1UHPN_7Bfl4fHhfPafr16eX1f061YxBn8JGCKpKtAC04JBZAcZqKzjSkhXaYMWsgtyUDAqsbBk1yHXOqRZY2qpiC3I1zd367nvA0MvGxUPqWrXYDUECFYzmHGIhnwq170LwaOXWu0b5H5lROaKSIxM5MpHA5B8qyWPb5X7-sGnQHJr2nKK-nHSMT-4cehm0w1ajcR51L03n_l_wC7YxesM</recordid><startdate>198307</startdate><enddate>198307</enddate><creator>Pazzagli, M.</creator><creator>Messeri, G.</creator><creator>Caldini, A.L.</creator><creator>Moneti, G.</creator><creator>Martinazzo, G.</creator><creator>Serio, M.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198307</creationdate><title>Preparation and evaluation of steroid chemiluminescent tracers</title><author>Pazzagli, M. ; Messeri, G. ; Caldini, A.L. ; Moneti, G. ; Martinazzo, G. ; Serio, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c338t-8b660a7ef8805481f68dfcf64e0735cde93fa82d7385e9f7fcf82c240c6e7f993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Animals</topic><topic>Cross Reactions</topic><topic>Hormones - analysis</topic><topic>Humans</topic><topic>Hydrogen Peroxide</topic><topic>Hydrogen-Ion Concentration</topic><topic>Immunoassay</topic><topic>Indicators and Reagents</topic><topic>Luminescent Measurements</topic><topic>Mass Spectrometry - methods</topic><topic>Peroxidases</topic><topic>Steroids - analysis</topic><topic>Structure-Activity Relationship</topic><toplevel>online_resources</toplevel><creatorcontrib>Pazzagli, M.</creatorcontrib><creatorcontrib>Messeri, G.</creatorcontrib><creatorcontrib>Caldini, A.L.</creatorcontrib><creatorcontrib>Moneti, G.</creatorcontrib><creatorcontrib>Martinazzo, G.</creatorcontrib><creatorcontrib>Serio, M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of steroid biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pazzagli, M.</au><au>Messeri, G.</au><au>Caldini, A.L.</au><au>Moneti, G.</au><au>Martinazzo, G.</au><au>Serio, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation and evaluation of steroid chemiluminescent tracers</atitle><jtitle>Journal of steroid biochemistry</jtitle><addtitle>J Steroid Biochem</addtitle><date>1983-07</date><risdate>1983</risdate><volume>19</volume><issue>1</issue><spage>407</spage><epage>412</epage><pages>407-412</pages><issn>0022-4731</issn><abstract>Six different isoluminol derivatives have been synthesized and evaluated as potential labels for conjugation to steroid molecules. The resulting steroid-chemiluminescent tracers have been investigated in terms of detection limit and affinity for the homologous antibody. The conjugation to the steroid molecule did not significantly affect the quantum yield of the chemiluminescent label. Consequently the detection limit was very similar using tracers with the same chemiluminescent label and different steroids and it was always at picomolar levels. The affinity of the chemiluminescent tracer versus the homologous antibody was evaluated using tritiated steroids as reference. The bridge and the chemiluminescent molecule could influence the affinity of the chemiluminescent tracer which could be higher, similar or lower than that of the tritiated steroid. In conclusion some isoluminol derivatives (i.e. ABEI or AEEI) can be considered as universal labelling compounds for steroids and their metabolites, because they possess a high light efficiency (similar to luminal itself) and favourable reaction conditions. Moreover the conjugation to the steroid molecule does not modify their chemiluminescent properties.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>6887878</pmid><doi>10.1016/0022-4731(83)90195-4</doi><tpages>6</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0022-4731 |
ispartof | Journal of steroid biochemistry, 1983-07, Vol.19 (1), p.407-412 |
issn | 0022-4731 |
language | eng |
recordid | cdi_proquest_miscellaneous_80630248 |
source | MEDLINE; Alma/SFX Local Collection |
subjects | Animals Cross Reactions Hormones - analysis Humans Hydrogen Peroxide Hydrogen-Ion Concentration Immunoassay Indicators and Reagents Luminescent Measurements Mass Spectrometry - methods Peroxidases Steroids - analysis Structure-Activity Relationship |
title | Preparation and evaluation of steroid chemiluminescent tracers |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T17%3A21%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Preparation%20and%20evaluation%20of%20steroid%20chemiluminescent%20tracers&rft.jtitle=Journal%20of%20steroid%20biochemistry&rft.au=Pazzagli,%20M.&rft.date=1983-07&rft.volume=19&rft.issue=1&rft.spage=407&rft.epage=412&rft.pages=407-412&rft.issn=0022-4731&rft_id=info:doi/10.1016/0022-4731(83)90195-4&rft_dat=%3Cproquest_cross%3E80630248%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=80630248&rft_id=info:pmid/6887878&rft_els_id=0022473183901954&rfr_iscdi=true |