Production and characterization of antipeptide antibodies against human O6-methylguanine-DNA methyltransferase

Four synthetic peptides from the sequence of human O6-methylguanine-DNA methyltransferase (MGMT), three corresponding to different hydrophilic regions and one corresponding to the sequence containing the alkyl acceptor residue cysteine 145, were used to immunize rabbits. The antibody against Peptide...

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Veröffentlicht in:	Cancer research (Chicago, Ill.) Ill.), 1991-07, Vol.51 (13), p.3339-3344
Hauptverfasser:	OSTROWSKI, L. E, PEGRAM, C. N, VON WRONSKI, M. A, HUMPHREY, P. A, XUANMIN HE, SHIOTA, S, MITRA, S, BRENT, T. P, BIGNER, D. D
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analytical, structural and metabolic biochemistry Antibodies - immunology Antibody Specificity Biological and medical sciences Blotting, Western Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Humans Methyltransferases - chemistry Methyltransferases - immunology Molecular Sequence Data O-Methylguanine-DNA Methyltransferase Peptides - chemical synthesis Peptides - immunology Precipitin Tests Solubility Species Specificity Transferases
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