[46] Lysoplasmalogenase: solubilization and partial purification from liver microsomes

This chapter focuses on the process of solubilization and purification of lysoplasmalogenase from liver microsomes. In this coupled enzyme assay, the aldehyde released during the hydrolysis of the vinyl ether bond of lysoplasmalogen is reduced to the alcohol by exogenously added alcohol dehydrogenas...

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Veröffentlicht in:	Methods in Enzymology 1991, Vol.197, p.483-490
Hauptverfasser:	Jurkowitz-Alexander, M.S., Horrocks, L.A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Chromatography - methods Chromatography, DEAE-Cellulose - methods Detergents Durapatite Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Glucosides Hydrolases - analysis Hydrolases - isolation & purification Hydrolases - metabolism Hydroxyapatites Indicators and Reagents Kinetics Male Microsomes, Liver - enzymology Rats Rats, Inbred Strains Solubility Spectrophotometry, Ultraviolet - methods
Online-Zugang:	Volltext
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