Photoaffinity labeling of two rat liver plasma membrane proteins with [32P]gamma-azidoanilido GTP in response to vasopressin. Immunologic identification as alpha subunits of the Gq class of G proteins

Two proteins have been identified in rat liver plasma membranes that bind a photoreactive GTP analogue, [32P]gamma-azidoanilido GTP, in response to incubation with the Ca(2+)-mobilizing agonist, vasopressin. The labeled proteins possess apparent molecular masses of 42 and 43 kDa. Their labeling requ...

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Veröffentlicht in:	The Journal of biological chemistry 1991-06, Vol.266 (18), p.11409-11412
Hauptverfasser:	WANGE, R. L, SMRCKA, A. V, STERNWEIS, P. C, EXTON, J. H
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Labels Analytical, structural and metabolic biochemistry Animals Azides - chemistry Binding and carrier proteins Biological and medical sciences Blotting, Western Calcium - metabolism Enzyme Activation Fundamental and applied biological sciences. Psychology GTP Phosphohydrolases - metabolism GTP-Binding Proteins - metabolism Guanosine Triphosphate - analogs & derivatives Guanosine Triphosphate - chemistry Liver - drug effects Liver - metabolism Male Membrane Proteins - metabolism Phosphatidylinositol Diacylglycerol-Lyase Phosphoric Diester Hydrolases - metabolism Photochemistry Precipitin Tests Proteins Rats Rats, Inbred Strains Receptors, Angiotensin - drug effects Receptors, Vasopressin Type C Phospholipases - metabolism Vasopressins - pharmacology
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