Modulation of mouse complement receptors 1 and 2 suppresses antibody responses in vivo
A mAb, 7G6, that binds to mouse CR1 and CR2 and down-modulates their expression on splenic B cells in vivo, was used to determine whether a decrease in CR1 and CR2 expression affects antibody responses to different T-dependent and T-independent Ag. Injection of mice with the mAb 7G6 prior to immuniz...
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Veröffentlicht in: | The Journal of immunology (1950) 1991-07, Vol.147 (1), p.224-230 |
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creator | Thyphronitis, G Kinoshita, T Inoue, K Schweinle, JE Tsokos, GC Metcalf, ES Finkelman, FD Balow, JE |
description | A mAb, 7G6, that binds to mouse CR1 and CR2 and down-modulates their expression on splenic B cells in vivo, was used to determine whether a decrease in CR1 and CR2 expression affects antibody responses to different T-dependent and T-independent Ag. Injection of mice with the mAb 7G6 prior to immunization with FITC haptenated Salmonella typhimurium (SH5771), Salmonella montevideo (SH5770), SRBC, or Ficoll dramatically decreased subsequent antibody responses to FITC. Although both IgM and IgG primary antibody responses were affected similarly, the antibody levels were most inhibited during early phases of the response. In contrast, down-modulation of the CR did not affect memory antibody responses, because injection of mice with 7G6 before a second immunization with FITC-SH5771 had no effect on subsequent anti-FITC antibody production. Moreover, polyclonal in vivo activation of the mouse immune system by anti-mouse IgD antibodies was not affected by previous administration of 7G6, because anti-IgD-induced increases in Ia expression and serum IgG1 levels were not affected. Taken together, these observations suggest that CR1 and CR2 may play an important role in enhancing primary antibody responses to many T-dependent and T-independent Ag and may contribute to a host's response to naturally occurring antigens such as bacteria. |
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Injection of mice with the mAb 7G6 prior to immunization with FITC haptenated Salmonella typhimurium (SH5771), Salmonella montevideo (SH5770), SRBC, or Ficoll dramatically decreased subsequent antibody responses to FITC. Although both IgM and IgG primary antibody responses were affected similarly, the antibody levels were most inhibited during early phases of the response. In contrast, down-modulation of the CR did not affect memory antibody responses, because injection of mice with 7G6 before a second immunization with FITC-SH5771 had no effect on subsequent anti-FITC antibody production. Moreover, polyclonal in vivo activation of the mouse immune system by anti-mouse IgD antibodies was not affected by previous administration of 7G6, because anti-IgD-induced increases in Ia expression and serum IgG1 levels were not affected. Taken together, these observations suggest that CR1 and CR2 may play an important role in enhancing primary antibody responses to many T-dependent and T-independent Ag and may contribute to a host's response to naturally occurring antigens such as bacteria.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.147.1.224</identifier><identifier>PMID: 1828822</identifier><identifier>CODEN: JOIMA3</identifier><language>eng</language><publisher>Bethesda, MD: Am Assoc Immnol</publisher><subject>Animals ; Antibody Formation ; Antigens, Differentiation - immunology ; Antigens, Differentiation, B-Lymphocyte - immunology ; Antigens, Differentiation, B-Lymphocyte - physiology ; B-Lymphocytes - immunology ; Biological and medical sciences ; Down-Regulation ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Immunobiology ; Immunoglobulin G - biosynthesis ; Immunoglobulin Isotypes - biosynthesis ; Immunoglobulin M - biosynthesis ; Immunologic Memory ; Lymphocyte Activation ; Mice ; Mice, Inbred Strains ; Modulation of the immune response (stimulation, suppression) ; Receptors, Complement - physiology ; Receptors, Complement 3b ; Receptors, Complement 3d ; Receptors, Fc - immunology ; Receptors, IgE ; Receptors, IgG</subject><ispartof>The Journal of immunology (1950), 1991-07, Vol.147 (1), p.224-230</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3484-f67288afab33983fa83469398e75189c21e5dce91656851c1fa3c7e671e60c3a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4944532$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1828822$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thyphronitis, G</creatorcontrib><creatorcontrib>Kinoshita, T</creatorcontrib><creatorcontrib>Inoue, K</creatorcontrib><creatorcontrib>Schweinle, JE</creatorcontrib><creatorcontrib>Tsokos, GC</creatorcontrib><creatorcontrib>Metcalf, ES</creatorcontrib><creatorcontrib>Finkelman, FD</creatorcontrib><creatorcontrib>Balow, JE</creatorcontrib><title>Modulation of mouse complement receptors 1 and 2 suppresses antibody responses in vivo</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>A mAb, 7G6, that binds to mouse CR1 and CR2 and down-modulates their expression on splenic B cells in vivo, was used to determine whether a decrease in CR1 and CR2 expression affects antibody responses to different T-dependent and T-independent Ag. Injection of mice with the mAb 7G6 prior to immunization with FITC haptenated Salmonella typhimurium (SH5771), Salmonella montevideo (SH5770), SRBC, or Ficoll dramatically decreased subsequent antibody responses to FITC. Although both IgM and IgG primary antibody responses were affected similarly, the antibody levels were most inhibited during early phases of the response. In contrast, down-modulation of the CR did not affect memory antibody responses, because injection of mice with 7G6 before a second immunization with FITC-SH5771 had no effect on subsequent anti-FITC antibody production. Moreover, polyclonal in vivo activation of the mouse immune system by anti-mouse IgD antibodies was not affected by previous administration of 7G6, because anti-IgD-induced increases in Ia expression and serum IgG1 levels were not affected. Taken together, these observations suggest that CR1 and CR2 may play an important role in enhancing primary antibody responses to many T-dependent and T-independent Ag and may contribute to a host's response to naturally occurring antigens such as bacteria.</description><subject>Animals</subject><subject>Antibody Formation</subject><subject>Antigens, Differentiation - immunology</subject><subject>Antigens, Differentiation, B-Lymphocyte - immunology</subject><subject>Antigens, Differentiation, B-Lymphocyte - physiology</subject><subject>B-Lymphocytes - immunology</subject><subject>Biological and medical sciences</subject><subject>Down-Regulation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Immunobiology</subject><subject>Immunoglobulin G - biosynthesis</subject><subject>Immunoglobulin Isotypes - biosynthesis</subject><subject>Immunoglobulin M - biosynthesis</subject><subject>Immunologic Memory</subject><subject>Lymphocyte Activation</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Modulation of the immune response (stimulation, suppression)</subject><subject>Receptors, Complement - physiology</subject><subject>Receptors, Complement 3b</subject><subject>Receptors, Complement 3d</subject><subject>Receptors, Fc - immunology</subject><subject>Receptors, IgE</subject><subject>Receptors, IgG</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v3CAQhlHVKt2m_QOVKnGoevOGAQz4WEXph5Qql6RXxOJxQ4SNC3ZW-fcl2m332BNo5pmX4SHkPbCtZLK7eAjjuE4pbkHqLWw5ly_IBtqWNUox9ZJsGOO8Aa30a_KmlAfGmGJcnpEzMNwYzjfk54_Ur9EtIU00DXRMa0Hq0zhHHHFaaEaP85JyoUDd1FNOyzrPGUvBUgtL2KX-qVJlTtNzKUz0MTymt-TV4GLBd8fznNx9ubq9_NZc33z9fvn5uvFCGtkMStc93OB2QnRGDM4Iqbp6Rd2C6TwHbHuPHahWmRY8DE54jUoDKuaFE-fk0yF3zun3imWxYygeY3QT1q9YwxTnWuj_gqBYx1uQFeQH0OdUSsbBzjmMLj9ZYPbZuv1r3VbrFmy1Xoc-HNPX3Yj9aeSgufY_HvuueBeH7CYfyj9MdlK2gp-WvA-_7vchoy2ji7GGgt3v96f3_gB75Jmz</recordid><startdate>19910701</startdate><enddate>19910701</enddate><creator>Thyphronitis, G</creator><creator>Kinoshita, T</creator><creator>Inoue, K</creator><creator>Schweinle, JE</creator><creator>Tsokos, GC</creator><creator>Metcalf, ES</creator><creator>Finkelman, FD</creator><creator>Balow, JE</creator><general>Am Assoc Immnol</general><general>American Association of Immunologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19910701</creationdate><title>Modulation of mouse complement receptors 1 and 2 suppresses antibody responses in vivo</title><author>Thyphronitis, G ; Kinoshita, T ; Inoue, K ; Schweinle, JE ; Tsokos, GC ; Metcalf, ES ; Finkelman, FD ; Balow, JE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3484-f67288afab33983fa83469398e75189c21e5dce91656851c1fa3c7e671e60c3a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Antibody Formation</topic><topic>Antigens, Differentiation - immunology</topic><topic>Antigens, Differentiation, B-Lymphocyte - immunology</topic><topic>Antigens, Differentiation, B-Lymphocyte - physiology</topic><topic>B-Lymphocytes - immunology</topic><topic>Biological and medical sciences</topic><topic>Down-Regulation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Immunobiology</topic><topic>Immunoglobulin G - biosynthesis</topic><topic>Immunoglobulin Isotypes - biosynthesis</topic><topic>Immunoglobulin M - biosynthesis</topic><topic>Immunologic Memory</topic><topic>Lymphocyte Activation</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Modulation of the immune response (stimulation, suppression)</topic><topic>Receptors, Complement - physiology</topic><topic>Receptors, Complement 3b</topic><topic>Receptors, Complement 3d</topic><topic>Receptors, Fc - immunology</topic><topic>Receptors, IgE</topic><topic>Receptors, IgG</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thyphronitis, G</creatorcontrib><creatorcontrib>Kinoshita, T</creatorcontrib><creatorcontrib>Inoue, K</creatorcontrib><creatorcontrib>Schweinle, JE</creatorcontrib><creatorcontrib>Tsokos, GC</creatorcontrib><creatorcontrib>Metcalf, ES</creatorcontrib><creatorcontrib>Finkelman, FD</creatorcontrib><creatorcontrib>Balow, JE</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thyphronitis, G</au><au>Kinoshita, T</au><au>Inoue, K</au><au>Schweinle, JE</au><au>Tsokos, GC</au><au>Metcalf, ES</au><au>Finkelman, FD</au><au>Balow, JE</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulation of mouse complement receptors 1 and 2 suppresses antibody responses in vivo</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1991-07-01</date><risdate>1991</risdate><volume>147</volume><issue>1</issue><spage>224</spage><epage>230</epage><pages>224-230</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><coden>JOIMA3</coden><abstract>A mAb, 7G6, that binds to mouse CR1 and CR2 and down-modulates their expression on splenic B cells in vivo, was used to determine whether a decrease in CR1 and CR2 expression affects antibody responses to different T-dependent and T-independent Ag. Injection of mice with the mAb 7G6 prior to immunization with FITC haptenated Salmonella typhimurium (SH5771), Salmonella montevideo (SH5770), SRBC, or Ficoll dramatically decreased subsequent antibody responses to FITC. Although both IgM and IgG primary antibody responses were affected similarly, the antibody levels were most inhibited during early phases of the response. In contrast, down-modulation of the CR did not affect memory antibody responses, because injection of mice with 7G6 before a second immunization with FITC-SH5771 had no effect on subsequent anti-FITC antibody production. Moreover, polyclonal in vivo activation of the mouse immune system by anti-mouse IgD antibodies was not affected by previous administration of 7G6, because anti-IgD-induced increases in Ia expression and serum IgG1 levels were not affected. 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subjects | Animals Antibody Formation Antigens, Differentiation - immunology Antigens, Differentiation, B-Lymphocyte - immunology Antigens, Differentiation, B-Lymphocyte - physiology B-Lymphocytes - immunology Biological and medical sciences Down-Regulation Fundamental and applied biological sciences. Psychology Fundamental immunology Immunobiology Immunoglobulin G - biosynthesis Immunoglobulin Isotypes - biosynthesis Immunoglobulin M - biosynthesis Immunologic Memory Lymphocyte Activation Mice Mice, Inbred Strains Modulation of the immune response (stimulation, suppression) Receptors, Complement - physiology Receptors, Complement 3b Receptors, Complement 3d Receptors, Fc - immunology Receptors, IgE Receptors, IgG |
title | Modulation of mouse complement receptors 1 and 2 suppresses antibody responses in vivo |
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