Cytogenetic and biochemical investigations of fibroblast cultures and clones with one and two active-X chromosomes of a 69, XXY triploidy
Bromodeoxyuridine replication patterns showed that fibroblasts from a 69,XXY triploidy carried either one or two early replicating X chromosomes. The activity of alpha-galactosidase A measured in single cells fell into two classes with a ratio of 1:2. Dilute plating produced clones of both types wit...
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| Veröffentlicht in: | Human genetics 1983-09, Vol.64 (3), p.246-248 |
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| container_end_page | 248 |
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| container_issue | 3 |
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| container_title | Human genetics |
| container_volume | 64 |
| creator | VOGEL, W TRAUTMANN, T HORLER, H PENTZ, S |
| description | Bromodeoxyuridine replication patterns showed that fibroblasts from a 69,XXY triploidy carried either one or two early replicating X chromosomes. The activity of alpha-galactosidase A measured in single cells fell into two classes with a ratio of 1:2. Dilute plating produced clones of both types with the activity of alpha-galactosidase A corresponding to the number of active X chromosomes. To our knowledge, this is the first report on clones of a triploidy with different numbers of active X chromosomes, and on a gene-dosage effect of an X-linked gene using triploid cells with one active X as control. |
| doi_str_mv | 10.1007/BF00279402 |
| format | Article |
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The activity of alpha-galactosidase A measured in single cells fell into two classes with a ratio of 1:2. Dilute plating produced clones of both types with the activity of alpha-galactosidase A corresponding to the number of active X chromosomes. To our knowledge, this is the first report on clones of a triploidy with different numbers of active X chromosomes, and on a gene-dosage effect of an X-linked gene using triploid cells with one active X as control.</description><identifier>ISSN: 0340-6717</identifier><identifier>EISSN: 1432-1203</identifier><identifier>DOI: 10.1007/BF00279402</identifier><identifier>PMID: 6309648</identifier><identifier>CODEN: HUGEDQ</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>alpha-Galactosidase - genetics ; Biological and medical sciences ; Cell Division ; Cells, Cultured ; Chromosome aberrations ; Clone Cells - cytology ; Dosage Compensation, Genetic ; Female ; Fibroblasts - cytology ; Galactosidases - genetics ; Humans ; In Vitro Techniques ; Infant, Newborn ; Klinefelter Syndrome - genetics ; Male ; Medical genetics ; Medical sciences ; Polyploidy ; X Chromosome</subject><ispartof>Human genetics, 1983-09, Vol.64 (3), p.246-248</ispartof><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c226t-d45995fe2f4e5fdd61e6ff6bd759b4055e7d911fa1156199b9a26b0809b72f813</citedby><cites>FETCH-LOGICAL-c226t-d45995fe2f4e5fdd61e6ff6bd759b4055e7d911fa1156199b9a26b0809b72f813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27925,27926</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9615689$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6309648$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>VOGEL, W</creatorcontrib><creatorcontrib>TRAUTMANN, T</creatorcontrib><creatorcontrib>HORLER, H</creatorcontrib><creatorcontrib>PENTZ, S</creatorcontrib><title>Cytogenetic and biochemical investigations of fibroblast cultures and clones with one and two active-X chromosomes of a 69, XXY triploidy</title><title>Human genetics</title><addtitle>Hum Genet</addtitle><description>Bromodeoxyuridine replication patterns showed that fibroblasts from a 69,XXY triploidy carried either one or two early replicating X chromosomes. The activity of alpha-galactosidase A measured in single cells fell into two classes with a ratio of 1:2. Dilute plating produced clones of both types with the activity of alpha-galactosidase A corresponding to the number of active X chromosomes. To our knowledge, this is the first report on clones of a triploidy with different numbers of active X chromosomes, and on a gene-dosage effect of an X-linked gene using triploid cells with one active X as control.</description><subject>alpha-Galactosidase - genetics</subject><subject>Biological and medical sciences</subject><subject>Cell Division</subject><subject>Cells, Cultured</subject><subject>Chromosome aberrations</subject><subject>Clone Cells - cytology</subject><subject>Dosage Compensation, Genetic</subject><subject>Female</subject><subject>Fibroblasts - cytology</subject><subject>Galactosidases - genetics</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Infant, Newborn</subject><subject>Klinefelter Syndrome - genetics</subject><subject>Male</subject><subject>Medical genetics</subject><subject>Medical sciences</subject><subject>Polyploidy</subject><subject>X Chromosome</subject><issn>0340-6717</issn><issn>1432-1203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEFPGzEQha2KCgLl0juSD6iHqtuOvWvv-kijUiohcQEpnFa2105c7a5T25soP4F_XZNEcJqnmW-eZh5Cnwl8JwD1j5-3ALQWFdAPaEaqkhaEQnmCZlBWUPCa1GfoPMa_AIQJyk7RKS9B8KqZoZf5LvmlGU1yGsuxw8p5vTKD07LHbtyYmNxSJufHiL3F1qngVS9jwnrq0xRM3G_p3o9Zbl1a4az2vbT1WOrkNqZYYL0KfvDRD2bvIzEX3_Bi8YxTcOveu273CX20so_m8lgv0NPtr8f5XXH_8PvP_Oa-0JTyVHQVE4JZQ21lmO06Tgy3lquuZkJVwJipO0GIlYQwToRQQlKuoAGhamobUl6gLwffdfD_pvxfO7ioTd_L0fgptg3ktZLTDH49gDr4GIOx7Tq4QYZdS6B9zb19zz3DV0fXSQ2me0OPQef59XEuY47WBjlqF98wwfO1jSj_A84YiyE</recordid><startdate>198309</startdate><enddate>198309</enddate><creator>VOGEL, W</creator><creator>TRAUTMANN, T</creator><creator>HORLER, H</creator><creator>PENTZ, S</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198309</creationdate><title>Cytogenetic and biochemical investigations of fibroblast cultures and clones with one and two active-X chromosomes of a 69, XXY triploidy</title><author>VOGEL, W ; TRAUTMANN, T ; HORLER, H ; PENTZ, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c226t-d45995fe2f4e5fdd61e6ff6bd759b4055e7d911fa1156199b9a26b0809b72f813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>alpha-Galactosidase - genetics</topic><topic>Biological and medical sciences</topic><topic>Cell Division</topic><topic>Cells, Cultured</topic><topic>Chromosome aberrations</topic><topic>Clone Cells - cytology</topic><topic>Dosage Compensation, Genetic</topic><topic>Female</topic><topic>Fibroblasts - cytology</topic><topic>Galactosidases - genetics</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Infant, Newborn</topic><topic>Klinefelter Syndrome - genetics</topic><topic>Male</topic><topic>Medical genetics</topic><topic>Medical sciences</topic><topic>Polyploidy</topic><topic>X Chromosome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>VOGEL, W</creatorcontrib><creatorcontrib>TRAUTMANN, T</creatorcontrib><creatorcontrib>HORLER, H</creatorcontrib><creatorcontrib>PENTZ, S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Human genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>VOGEL, W</au><au>TRAUTMANN, T</au><au>HORLER, H</au><au>PENTZ, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytogenetic and biochemical investigations of fibroblast cultures and clones with one and two active-X chromosomes of a 69, XXY triploidy</atitle><jtitle>Human genetics</jtitle><addtitle>Hum Genet</addtitle><date>1983-09</date><risdate>1983</risdate><volume>64</volume><issue>3</issue><spage>246</spage><epage>248</epage><pages>246-248</pages><issn>0340-6717</issn><eissn>1432-1203</eissn><coden>HUGEDQ</coden><abstract>Bromodeoxyuridine replication patterns showed that fibroblasts from a 69,XXY triploidy carried either one or two early replicating X chromosomes. The activity of alpha-galactosidase A measured in single cells fell into two classes with a ratio of 1:2. Dilute plating produced clones of both types with the activity of alpha-galactosidase A corresponding to the number of active X chromosomes. To our knowledge, this is the first report on clones of a triploidy with different numbers of active X chromosomes, and on a gene-dosage effect of an X-linked gene using triploid cells with one active X as control.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><cop>New York, NY</cop><pub>Springer</pub><pmid>6309648</pmid><doi>10.1007/BF00279402</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0340-6717 |
| ispartof | Human genetics, 1983-09, Vol.64 (3), p.246-248 |
| issn | 0340-6717 1432-1203 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | alpha-Galactosidase - genetics Biological and medical sciences Cell Division Cells, Cultured Chromosome aberrations Clone Cells - cytology Dosage Compensation, Genetic Female Fibroblasts - cytology Galactosidases - genetics Humans In Vitro Techniques Infant, Newborn Klinefelter Syndrome - genetics Male Medical genetics Medical sciences Polyploidy X Chromosome |
| title | Cytogenetic and biochemical investigations of fibroblast cultures and clones with one and two active-X chromosomes of a 69, XXY triploidy |
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