Effect of partially purified human somatomedin on human fetal and postnatal cartilage in vitro
Partly purified somatomedin prepared from adult human plasma stimulated [ 3h]thymidine and [ 35s]sulphate uptake into human cartilage. At a final concentration of 0.1 U/ml somatomedin the uptake of [ 3H]thymidine into fetal cartilage at 18 weeks of gestation was 162 ± 23% compared with controls. Som...
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| Veröffentlicht in: | Early human development 1983-07, Vol.8 (2), p.135-140 |
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| creator | Ashton, I.K. Spencer, E.M. |
| description | Partly purified somatomedin prepared from adult human plasma stimulated [
3h]thymidine and [
35s]sulphate uptake into human cartilage. At a final concentration of 0.1 U/ml somatomedin the uptake of [
3H]thymidine into fetal cartilage at 18 weeks of gestation was 162 ± 23% compared with controls. Somatomedin also increased the incorporation of [
35s]sulphate into fetal cartilage to 137 ± 22% at 18 weeks compared to controls.
The same concentration of somatomedin (0.1 U/ml) also increased the incorporation of [
3h]thymidine into postnatal articular cartilage to 239 ± 69% and [
35s]sulphate to 133 ± 28% compared to controls.
Human somatomedin prepared from adult plasma thus has a mitogenic effect on both fetal and postnatal cartilage. The smaller effect on fetal cartilage may reflect different forms of somatomedin and/or differences in receptors for somatomedins on adult and fetal chondrocytes. |
| doi_str_mv | 10.1016/0378-3782(83)90069-5 |
| format | Article |
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3h]thymidine and [
35s]sulphate uptake into human cartilage. At a final concentration of 0.1 U/ml somatomedin the uptake of [
3H]thymidine into fetal cartilage at 18 weeks of gestation was 162 ± 23% compared with controls. Somatomedin also increased the incorporation of [
35s]sulphate into fetal cartilage to 137 ± 22% at 18 weeks compared to controls.
The same concentration of somatomedin (0.1 U/ml) also increased the incorporation of [
3h]thymidine into postnatal articular cartilage to 239 ± 69% and [
35s]sulphate to 133 ± 28% compared to controls.
Human somatomedin prepared from adult plasma thus has a mitogenic effect on both fetal and postnatal cartilage. The smaller effect on fetal cartilage may reflect different forms of somatomedin and/or differences in receptors for somatomedins on adult and fetal chondrocytes.</description><identifier>ISSN: 0378-3782</identifier><identifier>EISSN: 1872-6232</identifier><identifier>DOI: 10.1016/0378-3782(83)90069-5</identifier><identifier>PMID: 6884255</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Adolescent ; Adult ; cartilage ; Cartilage - drug effects ; Cartilage - embryology ; Cartilage - metabolism ; Cartilage, Articular - drug effects ; Cartilage, Articular - metabolism ; Child ; Female ; fetus ; Fetus - drug effects ; Fetus - metabolism ; Growth ; Humans ; In Vitro Techniques ; Male ; somatomedin ; Somatomedins - pharmacology ; Sulfates - metabolism ; Thymidine - metabolism ; Tritium</subject><ispartof>Early human development, 1983-07, Vol.8 (2), p.135-140</ispartof><rights>1983</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-c49a4372caef0a52e09c07ed04006e4f253530cbf17b7b25c250daab421f61353</citedby><cites>FETCH-LOGICAL-c272t-c49a4372caef0a52e09c07ed04006e4f253530cbf17b7b25c250daab421f61353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0378378283900695$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6884255$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ashton, I.K.</creatorcontrib><creatorcontrib>Spencer, E.M.</creatorcontrib><title>Effect of partially purified human somatomedin on human fetal and postnatal cartilage in vitro</title><title>Early human development</title><addtitle>Early Hum Dev</addtitle><description>Partly purified somatomedin prepared from adult human plasma stimulated [
3h]thymidine and [
35s]sulphate uptake into human cartilage. At a final concentration of 0.1 U/ml somatomedin the uptake of [
3H]thymidine into fetal cartilage at 18 weeks of gestation was 162 ± 23% compared with controls. Somatomedin also increased the incorporation of [
35s]sulphate into fetal cartilage to 137 ± 22% at 18 weeks compared to controls.
The same concentration of somatomedin (0.1 U/ml) also increased the incorporation of [
3h]thymidine into postnatal articular cartilage to 239 ± 69% and [
35s]sulphate to 133 ± 28% compared to controls.
Human somatomedin prepared from adult plasma thus has a mitogenic effect on both fetal and postnatal cartilage. The smaller effect on fetal cartilage may reflect different forms of somatomedin and/or differences in receptors for somatomedins on adult and fetal chondrocytes.</description><subject>Adolescent</subject><subject>Adult</subject><subject>cartilage</subject><subject>Cartilage - drug effects</subject><subject>Cartilage - embryology</subject><subject>Cartilage - metabolism</subject><subject>Cartilage, Articular - drug effects</subject><subject>Cartilage, Articular - metabolism</subject><subject>Child</subject><subject>Female</subject><subject>fetus</subject><subject>Fetus - drug effects</subject><subject>Fetus - metabolism</subject><subject>Growth</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>somatomedin</subject><subject>Somatomedins - pharmacology</subject><subject>Sulfates - metabolism</subject><subject>Thymidine - metabolism</subject><subject>Tritium</subject><issn>0378-3782</issn><issn>1872-6232</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLxDAUhYMoOj7-gUJWoovqTdr0sRFkGB8w4Ea3hjS90Ujb1KQV_PemzuDSxSXcnJMTzkfIKYMrBiy_hrQokzj8okwvK4C8SsQOWbCy4EnOU75LFn-WA3IYwgcAiLKCfbKfl2XGhViQ15UxqEfqDB2UH61q2286TN4aiw19nzrV0-A6NboOG9tT128vDY6qpapv6ODC2Kt503NCq96QRueXHb07JntGtQFPtucReblbPS8fkvXT_ePydp1oXvAx0VmlsrTgWqEBJThCpaHABrJYCzPDRSpS0LVhRV3UXGguoFGqzjgzOYvaETnf5A7efU4YRtnZoLFtVY9uCrKEnBUc8mjMNkbtXQgejRy87ZT_lgzkjFXOzOTMTJap_MUq5_yzbf5URxB_j7Yco36z0TGW_LLoZdAWex2Z-YhXNs7-_8EPL3iHlw</recordid><startdate>198307</startdate><enddate>198307</enddate><creator>Ashton, I.K.</creator><creator>Spencer, E.M.</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198307</creationdate><title>Effect of partially purified human somatomedin on human fetal and postnatal cartilage in vitro</title><author>Ashton, I.K. ; Spencer, E.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-c49a4372caef0a52e09c07ed04006e4f253530cbf17b7b25c250daab421f61353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>cartilage</topic><topic>Cartilage - drug effects</topic><topic>Cartilage - embryology</topic><topic>Cartilage - metabolism</topic><topic>Cartilage, Articular - drug effects</topic><topic>Cartilage, Articular - metabolism</topic><topic>Child</topic><topic>Female</topic><topic>fetus</topic><topic>Fetus - drug effects</topic><topic>Fetus - metabolism</topic><topic>Growth</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>somatomedin</topic><topic>Somatomedins - pharmacology</topic><topic>Sulfates - metabolism</topic><topic>Thymidine - metabolism</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ashton, I.K.</creatorcontrib><creatorcontrib>Spencer, E.M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Early human development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ashton, I.K.</au><au>Spencer, E.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of partially purified human somatomedin on human fetal and postnatal cartilage in vitro</atitle><jtitle>Early human development</jtitle><addtitle>Early Hum Dev</addtitle><date>1983-07</date><risdate>1983</risdate><volume>8</volume><issue>2</issue><spage>135</spage><epage>140</epage><pages>135-140</pages><issn>0378-3782</issn><eissn>1872-6232</eissn><abstract>Partly purified somatomedin prepared from adult human plasma stimulated [
3h]thymidine and [
35s]sulphate uptake into human cartilage. At a final concentration of 0.1 U/ml somatomedin the uptake of [
3H]thymidine into fetal cartilage at 18 weeks of gestation was 162 ± 23% compared with controls. Somatomedin also increased the incorporation of [
35s]sulphate into fetal cartilage to 137 ± 22% at 18 weeks compared to controls.
The same concentration of somatomedin (0.1 U/ml) also increased the incorporation of [
3h]thymidine into postnatal articular cartilage to 239 ± 69% and [
35s]sulphate to 133 ± 28% compared to controls.
Human somatomedin prepared from adult plasma thus has a mitogenic effect on both fetal and postnatal cartilage. The smaller effect on fetal cartilage may reflect different forms of somatomedin and/or differences in receptors for somatomedins on adult and fetal chondrocytes.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>6884255</pmid><doi>10.1016/0378-3782(83)90069-5</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-3782 |
| ispartof | Early human development, 1983-07, Vol.8 (2), p.135-140 |
| issn | 0378-3782 1872-6232 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80617206 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adolescent Adult cartilage Cartilage - drug effects Cartilage - embryology Cartilage - metabolism Cartilage, Articular - drug effects Cartilage, Articular - metabolism Child Female fetus Fetus - drug effects Fetus - metabolism Growth Humans In Vitro Techniques Male somatomedin Somatomedins - pharmacology Sulfates - metabolism Thymidine - metabolism Tritium |
| title | Effect of partially purified human somatomedin on human fetal and postnatal cartilage in vitro |
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