Reactivation of isolated mitotic apparatus: Metaphase versus anaphase spindles

Mitotic spindles isolated from sea urchin eggs can be reactivated to undergo mitotic processes in vitro. Spindles incubated in reactivation media containing sea urchin tubulin and nucleotides undergo pole‐pole elongation similar to that observed in living cells during anaphase‐B. The in vitro behavi...

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Veröffentlicht in:	Cell motility and the cytoskeleton 1991, Vol.18 (4), p.304-318
Hauptverfasser:	Palazzo, Robert E., Lutz, Douglas A., Rebhun, Lionel I.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - metabolism Anaphase - physiology Animals Biological and medical sciences Birefringence Cell physiology chromosome Chromosomes - physiology Fundamental and applied biological sciences. Psychology Guanosine Triphosphate - metabolism Metaphase - physiology Microscopy, Polarization mitosis Molecular and cellular biology Ovum Sea Urchins spindle Spindle Apparatus - metabolism Time Factors Tubulin - metabolism
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