Increased yield of a lysozyme after self-cloning of the gene in Streptomyces coelicolor Müller
Streptomyces coelicolor "Müller" DSM3030 excretes a lysozyme comprising both beta-1,4-N-acetyl- and beta-1,4-N,6-O-diacetyl muramidase activities. The lysozyme is named Cellosyl. Gene libraries have been established using genomic DNA from the wild-type strain, S. coelicolor DSM3030, and fr...
Gespeichert in:
| Veröffentlicht in: | Applied microbiology and biotechnology 1991, Vol.34 (4), p.481-487 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 487 |
|---|---|
| container_issue | 4 |
| container_start_page | 481 |
| container_title | Applied microbiology and biotechnology |
| container_volume | 34 |
| creator | BRAÊU, B HILGENFELD, R SCHLINGMANN, M MARQUARDT, R BIRR, E WOHLLEBEN, W AUFDERHEIDE, K PUÊHLER, A |
| description | Streptomyces coelicolor "Müller" DSM3030 excretes a lysozyme comprising both beta-1,4-N-acetyl- and beta-1,4-N,6-O-diacetyl muramidase activities. The lysozyme is named Cellosyl. Gene libraries have been established using genomic DNA from the wild-type strain, S. coelicolor DSM3030, and from an overproducing mutant, S. coelicolor HP1, which exhibits about a twofold increase in lysozyme production. The lysozyme-encoding genes (cel) from both strains were detected by oligodeoxynucleotide hybridization. The nucleotide sequence of the cel genes isolated from both strains was shown to be identical. The different levels of lysozyme production could not be correlated with any mutations at the cel gene locus. The cel gene isolated from the wild-type strain could not be expressed in some other species of Streptomyces. However, self-cloning of the cel gene into S. coelicolor DSM3030 and HP1 resulted in a 2.5-fold increase in lysozyme production. |
| doi_str_mv | 10.1007/BF00180575 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_80611014</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>80611014</sourcerecordid><originalsourceid>FETCH-LOGICAL-p236t-f3393734c522f3ced72b1716219bc217e765c06ec44763a953e7c6a584bc7f63</originalsourceid><addsrcrecordid>eNpFkL1OwzAYRS0EKqWwsCN5gS3gn9hORqgoVCpioHvkOJ9LkBMHOx3SZ2PjxQgiEtMdztEdDkKXlNxSQtTdw4oQmhGhxBGa05SzhEiaHqM5oUokSuTZKTqL8WO0WCblDM0ol4pxMkfFujUBdIQKDzW4CnuLNXZD9IehAaxtDwFHcDYxzrd1u_sV-nfAO2gB1y1-6wN0vW8GAxEbD6423vmAX76_nINwjk6sdhEupl2g7epxu3xONq9P6-X9JukYl31iOc-54qkRjFluoFKspIpKRvPSMKpASWGIBJOmSnKdCw7KSC2ytDTKSr5AN3-3XfCfe4h90dTRgHO6Bb-PRTYGoWRMs0BXk7gvG6iKLtSNDkMxBRn59cR1NNrZoFtTx38tVyzLVc5_AIaHbqg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>80611014</pqid></control><display><type>article</type><title>Increased yield of a lysozyme after self-cloning of the gene in Streptomyces coelicolor Müller</title><source>MEDLINE</source><source>SpringerLink</source><creator>BRAÊU, B ; HILGENFELD, R ; SCHLINGMANN, M ; MARQUARDT, R ; BIRR, E ; WOHLLEBEN, W ; AUFDERHEIDE, K ; PUÊHLER, A</creator><creatorcontrib>BRAÊU, B ; HILGENFELD, R ; SCHLINGMANN, M ; MARQUARDT, R ; BIRR, E ; WOHLLEBEN, W ; AUFDERHEIDE, K ; PUÊHLER, A</creatorcontrib><description>Streptomyces coelicolor "Müller" DSM3030 excretes a lysozyme comprising both beta-1,4-N-acetyl- and beta-1,4-N,6-O-diacetyl muramidase activities. The lysozyme is named Cellosyl. Gene libraries have been established using genomic DNA from the wild-type strain, S. coelicolor DSM3030, and from an overproducing mutant, S. coelicolor HP1, which exhibits about a twofold increase in lysozyme production. The lysozyme-encoding genes (cel) from both strains were detected by oligodeoxynucleotide hybridization. The nucleotide sequence of the cel genes isolated from both strains was shown to be identical. The different levels of lysozyme production could not be correlated with any mutations at the cel gene locus. The cel gene isolated from the wild-type strain could not be expressed in some other species of Streptomyces. However, self-cloning of the cel gene into S. coelicolor DSM3030 and HP1 resulted in a 2.5-fold increase in lysozyme production.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/BF00180575</identifier><identifier>PMID: 1367230</identifier><identifier>CODEN: AMBIDG</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Amino Acid Sequence ; Base Sequence ; Biological and medical sciences ; Biotechnology ; Cloning, Molecular ; DNA, Bacterial - chemistry ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; Genetic engineering ; Genetic technics ; Genomic Library ; Methods. Procedures. Technologies ; Molecular cloning ; Molecular Sequence Data ; Muramidase - biosynthesis ; Muramidase - genetics ; Restriction Mapping ; Streptomyces - enzymology ; Streptomyces - genetics</subject><ispartof>Applied microbiology and biotechnology, 1991, Vol.34 (4), p.481-487</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19728979$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1367230$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BRAÊU, B</creatorcontrib><creatorcontrib>HILGENFELD, R</creatorcontrib><creatorcontrib>SCHLINGMANN, M</creatorcontrib><creatorcontrib>MARQUARDT, R</creatorcontrib><creatorcontrib>BIRR, E</creatorcontrib><creatorcontrib>WOHLLEBEN, W</creatorcontrib><creatorcontrib>AUFDERHEIDE, K</creatorcontrib><creatorcontrib>PUÊHLER, A</creatorcontrib><title>Increased yield of a lysozyme after self-cloning of the gene in Streptomyces coelicolor Müller</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><description>Streptomyces coelicolor "Müller" DSM3030 excretes a lysozyme comprising both beta-1,4-N-acetyl- and beta-1,4-N,6-O-diacetyl muramidase activities. The lysozyme is named Cellosyl. Gene libraries have been established using genomic DNA from the wild-type strain, S. coelicolor DSM3030, and from an overproducing mutant, S. coelicolor HP1, which exhibits about a twofold increase in lysozyme production. The lysozyme-encoding genes (cel) from both strains were detected by oligodeoxynucleotide hybridization. The nucleotide sequence of the cel genes isolated from both strains was shown to be identical. The different levels of lysozyme production could not be correlated with any mutations at the cel gene locus. The cel gene isolated from the wild-type strain could not be expressed in some other species of Streptomyces. However, self-cloning of the cel gene into S. coelicolor DSM3030 and HP1 resulted in a 2.5-fold increase in lysozyme production.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cloning, Molecular</subject><subject>DNA, Bacterial - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genomic Library</subject><subject>Methods. Procedures. Technologies</subject><subject>Molecular cloning</subject><subject>Molecular Sequence Data</subject><subject>Muramidase - biosynthesis</subject><subject>Muramidase - genetics</subject><subject>Restriction Mapping</subject><subject>Streptomyces - enzymology</subject><subject>Streptomyces - genetics</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkL1OwzAYRS0EKqWwsCN5gS3gn9hORqgoVCpioHvkOJ9LkBMHOx3SZ2PjxQgiEtMdztEdDkKXlNxSQtTdw4oQmhGhxBGa05SzhEiaHqM5oUokSuTZKTqL8WO0WCblDM0ol4pxMkfFujUBdIQKDzW4CnuLNXZD9IehAaxtDwFHcDYxzrd1u_sV-nfAO2gB1y1-6wN0vW8GAxEbD6423vmAX76_nINwjk6sdhEupl2g7epxu3xONq9P6-X9JukYl31iOc-54qkRjFluoFKspIpKRvPSMKpASWGIBJOmSnKdCw7KSC2ytDTKSr5AN3-3XfCfe4h90dTRgHO6Bb-PRTYGoWRMs0BXk7gvG6iKLtSNDkMxBRn59cR1NNrZoFtTx38tVyzLVc5_AIaHbqg</recordid><startdate>1991</startdate><enddate>1991</enddate><creator>BRAÊU, B</creator><creator>HILGENFELD, R</creator><creator>SCHLINGMANN, M</creator><creator>MARQUARDT, R</creator><creator>BIRR, E</creator><creator>WOHLLEBEN, W</creator><creator>AUFDERHEIDE, K</creator><creator>PUÊHLER, A</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>1991</creationdate><title>Increased yield of a lysozyme after self-cloning of the gene in Streptomyces coelicolor Müller</title><author>BRAÊU, B ; HILGENFELD, R ; SCHLINGMANN, M ; MARQUARDT, R ; BIRR, E ; WOHLLEBEN, W ; AUFDERHEIDE, K ; PUÊHLER, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p236t-f3393734c522f3ced72b1716219bc217e765c06ec44763a953e7c6a584bc7f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cloning, Molecular</topic><topic>DNA, Bacterial - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genomic Library</topic><topic>Methods. Procedures. Technologies</topic><topic>Molecular cloning</topic><topic>Molecular Sequence Data</topic><topic>Muramidase - biosynthesis</topic><topic>Muramidase - genetics</topic><topic>Restriction Mapping</topic><topic>Streptomyces - enzymology</topic><topic>Streptomyces - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BRAÊU, B</creatorcontrib><creatorcontrib>HILGENFELD, R</creatorcontrib><creatorcontrib>SCHLINGMANN, M</creatorcontrib><creatorcontrib>MARQUARDT, R</creatorcontrib><creatorcontrib>BIRR, E</creatorcontrib><creatorcontrib>WOHLLEBEN, W</creatorcontrib><creatorcontrib>AUFDERHEIDE, K</creatorcontrib><creatorcontrib>PUÊHLER, A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BRAÊU, B</au><au>HILGENFELD, R</au><au>SCHLINGMANN, M</au><au>MARQUARDT, R</au><au>BIRR, E</au><au>WOHLLEBEN, W</au><au>AUFDERHEIDE, K</au><au>PUÊHLER, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased yield of a lysozyme after self-cloning of the gene in Streptomyces coelicolor Müller</atitle><jtitle>Applied microbiology and biotechnology</jtitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>1991</date><risdate>1991</risdate><volume>34</volume><issue>4</issue><spage>481</spage><epage>487</epage><pages>481-487</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><coden>AMBIDG</coden><abstract>Streptomyces coelicolor "Müller" DSM3030 excretes a lysozyme comprising both beta-1,4-N-acetyl- and beta-1,4-N,6-O-diacetyl muramidase activities. The lysozyme is named Cellosyl. Gene libraries have been established using genomic DNA from the wild-type strain, S. coelicolor DSM3030, and from an overproducing mutant, S. coelicolor HP1, which exhibits about a twofold increase in lysozyme production. The lysozyme-encoding genes (cel) from both strains were detected by oligodeoxynucleotide hybridization. The nucleotide sequence of the cel genes isolated from both strains was shown to be identical. The different levels of lysozyme production could not be correlated with any mutations at the cel gene locus. The cel gene isolated from the wild-type strain could not be expressed in some other species of Streptomyces. However, self-cloning of the cel gene into S. coelicolor DSM3030 and HP1 resulted in a 2.5-fold increase in lysozyme production.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>1367230</pmid><doi>10.1007/BF00180575</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0175-7598 |
| ispartof | Applied microbiology and biotechnology, 1991, Vol.34 (4), p.481-487 |
| issn | 0175-7598 1432-0614 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80611014 |
| source | MEDLINE; SpringerLink |
| subjects | Amino Acid Sequence Base Sequence Biological and medical sciences Biotechnology Cloning, Molecular DNA, Bacterial - chemistry Fundamental and applied biological sciences. Psychology Genes, Bacterial Genetic engineering Genetic technics Genomic Library Methods. Procedures. Technologies Molecular cloning Molecular Sequence Data Muramidase - biosynthesis Muramidase - genetics Restriction Mapping Streptomyces - enzymology Streptomyces - genetics |
| title | Increased yield of a lysozyme after self-cloning of the gene in Streptomyces coelicolor Müller |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T21%3A22%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Increased%20yield%20of%20a%20lysozyme%20after%20self-cloning%20of%20the%20gene%20in%20Streptomyces%20coelicolor%20M%C3%BCller&rft.jtitle=Applied%20microbiology%20and%20biotechnology&rft.au=BRA%C3%8AU,%20B&rft.date=1991&rft.volume=34&rft.issue=4&rft.spage=481&rft.epage=487&rft.pages=481-487&rft.issn=0175-7598&rft.eissn=1432-0614&rft.coden=AMBIDG&rft_id=info:doi/10.1007/BF00180575&rft_dat=%3Cproquest_pubme%3E80611014%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=80611014&rft_id=info:pmid/1367230&rfr_iscdi=true |