Pseudorabies virus infections in explants of porcine nasal mucosa
The spread of infection and the morphogenesis of three pseudorabies virus strains were studied in explants of porcine nasal mucosa. Virulent nia-3 virus was compared with a deletion mutant 2 · 4N3A, and with a non-virulent Bartha virus. All three virus strains infected nasal epithelial cells. nia-3...
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| Veröffentlicht in: | Research in veterinary science 1991, Vol.50 (1), p.45-53 |
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| creator | Pol, J.M.A. Quint, W.G.V. Kok, G.L. Broekhuysen-Davies, J.M. |
| description | The spread of infection and the morphogenesis of three pseudorabies virus strains were studied in explants of porcine nasal mucosa. Virulent
nia-3 virus was compared with a deletion mutant 2 · 4N3A, and with a non-virulent Bartha virus. All three virus strains infected nasal epithelial cells.
nia-3 virus particles were enveloped mainly at the inner nuclear membrane; the virus rapidly invaded the stroma, causing widespread necrosis. In contrast, 2 · 4N3A virus particles were enveloped mainly at the endoplasmic reticulum and the infection spread more slowly. Bartha virus particles were enveloped mainly at the endoplasmic reticulum; the infection spread slowly and remained restricted to the epithelial cells. In Situ
dna hybridisation showed an accumulation of Bartha virus DNA in the nucleus 24 hours after inoculation. In nasal mucosa viral virulence seemed directly related to the speed of replication and release of virus from infected cells. |
| doi_str_mv | 10.1016/0034-5288(91)90052-P |
| format | Article |
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nia-3 virus was compared with a deletion mutant 2 · 4N3A, and with a non-virulent Bartha virus. All three virus strains infected nasal epithelial cells.
nia-3 virus particles were enveloped mainly at the inner nuclear membrane; the virus rapidly invaded the stroma, causing widespread necrosis. In contrast, 2 · 4N3A virus particles were enveloped mainly at the endoplasmic reticulum and the infection spread more slowly. Bartha virus particles were enveloped mainly at the endoplasmic reticulum; the infection spread slowly and remained restricted to the epithelial cells. In Situ
dna hybridisation showed an accumulation of Bartha virus DNA in the nucleus 24 hours after inoculation. In nasal mucosa viral virulence seemed directly related to the speed of replication and release of virus from infected cells.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/0034-5288(91)90052-P</identifier><identifier>PMID: 1646473</identifier><language>eng</language><publisher>England: Elsevier India Pvt Ltd</publisher><subject>Animals ; Antigens, Viral - analysis ; Culture Techniques ; DNA, Viral - analysis ; Herpesvirus 1, Suid - growth & development ; Herpesvirus 1, Suid - pathogenicity ; Herpesvirus 1, Suid - ultrastructure ; Microscopy, Electron ; Nasal Mucosa - microbiology ; Nasal Mucosa - ultrastructure ; Nucleic Acid Hybridization ; Swine ; Virulence</subject><ispartof>Research in veterinary science, 1991, Vol.50 (1), p.45-53</ispartof><rights>1991</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-f760735d5fdf0259936ee58910f813717278e167a140e7a9b49d0a91f4d414103</citedby><cites>FETCH-LOGICAL-c388t-f760735d5fdf0259936ee58910f813717278e167a140e7a9b49d0a91f4d414103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0034-5288(91)90052-P$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1646473$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pol, J.M.A.</creatorcontrib><creatorcontrib>Quint, W.G.V.</creatorcontrib><creatorcontrib>Kok, G.L.</creatorcontrib><creatorcontrib>Broekhuysen-Davies, J.M.</creatorcontrib><title>Pseudorabies virus infections in explants of porcine nasal mucosa</title><title>Research in veterinary science</title><addtitle>Res Vet Sci</addtitle><description>The spread of infection and the morphogenesis of three pseudorabies virus strains were studied in explants of porcine nasal mucosa. Virulent
nia-3 virus was compared with a deletion mutant 2 · 4N3A, and with a non-virulent Bartha virus. All three virus strains infected nasal epithelial cells.
nia-3 virus particles were enveloped mainly at the inner nuclear membrane; the virus rapidly invaded the stroma, causing widespread necrosis. In contrast, 2 · 4N3A virus particles were enveloped mainly at the endoplasmic reticulum and the infection spread more slowly. Bartha virus particles were enveloped mainly at the endoplasmic reticulum; the infection spread slowly and remained restricted to the epithelial cells. In Situ
dna hybridisation showed an accumulation of Bartha virus DNA in the nucleus 24 hours after inoculation. In nasal mucosa viral virulence seemed directly related to the speed of replication and release of virus from infected cells.</description><subject>Animals</subject><subject>Antigens, Viral - analysis</subject><subject>Culture Techniques</subject><subject>DNA, Viral - analysis</subject><subject>Herpesvirus 1, Suid - growth & development</subject><subject>Herpesvirus 1, Suid - pathogenicity</subject><subject>Herpesvirus 1, Suid - ultrastructure</subject><subject>Microscopy, Electron</subject><subject>Nasal Mucosa - microbiology</subject><subject>Nasal Mucosa - ultrastructure</subject><subject>Nucleic Acid Hybridization</subject><subject>Swine</subject><subject>Virulence</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLAzEQx4MotT6-gcKeRA-rM5tsHhdBxBcI9qDnkO5OINJuatIV_fZubdGbnmbg_5jhx9gRwjkCygsALsq60vrU4JkBqKtyssXGWPOqrKTEbTb-seyyvZxfAUAgqhEboRRSKD5mV5NMfRuTmwbKxXtIfS5C56lZhtit1oI-FjPXLXMRfbGIqQkdFZ3LblbM-yZmd8B2vJtlOtzMffZye_N8fV8-Pt09XF89lg3Xell6JUHxuq1966GqjeGSqNYGwWvkClWlNKFUDgWQcmYqTAvOoBetQIHA99nJuneR4ltPeWnnITc0G56j2GerQSLoWvxrRAmoeMUHo1gbmxRzTuTtIoW5S58Wwa4Q2xU_u-JnDdpvxHYyxI43_f10Tu1vaM100C_XOg003gMlm5tAXUNtSANX28bw94EvhGGJPw</recordid><startdate>1991</startdate><enddate>1991</enddate><creator>Pol, J.M.A.</creator><creator>Quint, W.G.V.</creator><creator>Kok, G.L.</creator><creator>Broekhuysen-Davies, J.M.</creator><general>Elsevier India Pvt Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>1991</creationdate><title>Pseudorabies virus infections in explants of porcine nasal mucosa</title><author>Pol, J.M.A. ; Quint, W.G.V. ; Kok, G.L. ; Broekhuysen-Davies, J.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-f760735d5fdf0259936ee58910f813717278e167a140e7a9b49d0a91f4d414103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Antigens, Viral - analysis</topic><topic>Culture Techniques</topic><topic>DNA, Viral - analysis</topic><topic>Herpesvirus 1, Suid - growth & development</topic><topic>Herpesvirus 1, Suid - pathogenicity</topic><topic>Herpesvirus 1, Suid - ultrastructure</topic><topic>Microscopy, Electron</topic><topic>Nasal Mucosa - microbiology</topic><topic>Nasal Mucosa - ultrastructure</topic><topic>Nucleic Acid Hybridization</topic><topic>Swine</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pol, J.M.A.</creatorcontrib><creatorcontrib>Quint, W.G.V.</creatorcontrib><creatorcontrib>Kok, G.L.</creatorcontrib><creatorcontrib>Broekhuysen-Davies, J.M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Research in veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pol, J.M.A.</au><au>Quint, W.G.V.</au><au>Kok, G.L.</au><au>Broekhuysen-Davies, J.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pseudorabies virus infections in explants of porcine nasal mucosa</atitle><jtitle>Research in veterinary science</jtitle><addtitle>Res Vet Sci</addtitle><date>1991</date><risdate>1991</risdate><volume>50</volume><issue>1</issue><spage>45</spage><epage>53</epage><pages>45-53</pages><issn>0034-5288</issn><eissn>1532-2661</eissn><abstract>The spread of infection and the morphogenesis of three pseudorabies virus strains were studied in explants of porcine nasal mucosa. Virulent
nia-3 virus was compared with a deletion mutant 2 · 4N3A, and with a non-virulent Bartha virus. All three virus strains infected nasal epithelial cells.
nia-3 virus particles were enveloped mainly at the inner nuclear membrane; the virus rapidly invaded the stroma, causing widespread necrosis. In contrast, 2 · 4N3A virus particles were enveloped mainly at the endoplasmic reticulum and the infection spread more slowly. Bartha virus particles were enveloped mainly at the endoplasmic reticulum; the infection spread slowly and remained restricted to the epithelial cells. In Situ
dna hybridisation showed an accumulation of Bartha virus DNA in the nucleus 24 hours after inoculation. In nasal mucosa viral virulence seemed directly related to the speed of replication and release of virus from infected cells.</abstract><cop>England</cop><pub>Elsevier India Pvt Ltd</pub><pmid>1646473</pmid><doi>10.1016/0034-5288(91)90052-P</doi><tpages>9</tpages></addata></record> |
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| ispartof | Research in veterinary science, 1991, Vol.50 (1), p.45-53 |
| issn | 0034-5288 1532-2661 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Antigens, Viral - analysis Culture Techniques DNA, Viral - analysis Herpesvirus 1, Suid - growth & development Herpesvirus 1, Suid - pathogenicity Herpesvirus 1, Suid - ultrastructure Microscopy, Electron Nasal Mucosa - microbiology Nasal Mucosa - ultrastructure Nucleic Acid Hybridization Swine Virulence |
| title | Pseudorabies virus infections in explants of porcine nasal mucosa |
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