Isolation, characterization, and chromosomal mapping of mouse P450 17α-hydroxylase/C17-20 lyase
Cytochrome P450 17 alpha-hydroxylase/C17-20 lyase (P45017 alpha) catalyzes the conversion of C-21 steroids to C-19 steroids in gonads. A full-length mouse cDNA encoding P450 17 alpha was isolated from a mouse Leydig cell library and characterized by restriction mapping and sequencing. The predicted...
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| Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 1991-05, Vol.10 (1), p.270-275 |
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| creator | YOUNGBLOOD, G. L SARTORIUS, C TAYLOR, B. A PAYNE, A. H |
| description | Cytochrome P450 17 alpha-hydroxylase/C17-20 lyase (P45017 alpha) catalyzes the conversion of C-21 steroids to C-19 steroids in gonads. A full-length mouse cDNA encoding P450 17 alpha was isolated from a mouse Leydig cell library and characterized by restriction mapping and sequencing. The predicted amino acid sequence has 83% homology to rat, 66% homology to human, and 62% homology to bovine P45017 alpha amino acid sequences. The protein is 507 amino acids in length, which is 1 amino acid shorter than the human protein and 2 amino acids shorter than the bovine protein. The structural gene encoding P450 17 alpha (Cyp17) was localized utilizing an interspecific testcross to mouse chromosome 19, distal to Got-1. Another cytochrome P450, P4502c (Cyp2c), also is located at the distal end of chromosome 19. CYP17, CYP2c, and GOT1 have been mapped to human chromosome 10, with CYP2C and GOT1 mapped to the distal region, q24.3 and q25.3, respectively. The data in the present study indicate conserved syntenic loci on mouse chromosome 19 and human chromosome 10 and predict that the structural gene encoding P45017 alpha will be found distal to GOT1 on human chromosome 10. |
| doi_str_mv | 10.1016/0888-7543(91)90511-C |
| format | Article |
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L ; SARTORIUS, C ; TAYLOR, B. A ; PAYNE, A. H</creator><creatorcontrib>YOUNGBLOOD, G. L ; SARTORIUS, C ; TAYLOR, B. A ; PAYNE, A. H</creatorcontrib><description>Cytochrome P450 17 alpha-hydroxylase/C17-20 lyase (P45017 alpha) catalyzes the conversion of C-21 steroids to C-19 steroids in gonads. A full-length mouse cDNA encoding P450 17 alpha was isolated from a mouse Leydig cell library and characterized by restriction mapping and sequencing. The predicted amino acid sequence has 83% homology to rat, 66% homology to human, and 62% homology to bovine P45017 alpha amino acid sequences. The protein is 507 amino acids in length, which is 1 amino acid shorter than the human protein and 2 amino acids shorter than the bovine protein. The structural gene encoding P450 17 alpha (Cyp17) was localized utilizing an interspecific testcross to mouse chromosome 19, distal to Got-1. Another cytochrome P450, P4502c (Cyp2c), also is located at the distal end of chromosome 19. CYP17, CYP2c, and GOT1 have been mapped to human chromosome 10, with CYP2C and GOT1 mapped to the distal region, q24.3 and q25.3, respectively. The data in the present study indicate conserved syntenic loci on mouse chromosome 19 and human chromosome 10 and predict that the structural gene encoding P45017 alpha will be found distal to GOT1 on human chromosome 10.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1016/0888-7543(91)90511-C</identifier><identifier>PMID: 1840559</identifier><language>eng</language><publisher>San Diego, CA: Elsevier</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Blotting, Southern ; Crosses, Genetic ; DNA ; Fundamental and applied biological sciences. Psychology ; Genes. Genome ; Genetic Linkage ; Humans ; Leydig Cells ; Male ; Mice ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Restriction Mapping ; Sequence Homology, Nucleic Acid ; Steroid 17-alpha-Hydroxylase - genetics</subject><ispartof>Genomics (San Diego, Calif.), 1991-05, Vol.10 (1), p.270-275</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-76d3804e5381d02b14605fdfc131fe300426c920946e957bf5afc0f813e110f3</citedby><cites>FETCH-LOGICAL-c378t-76d3804e5381d02b14605fdfc131fe300426c920946e957bf5afc0f813e110f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19623306$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1840559$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>YOUNGBLOOD, G. L</creatorcontrib><creatorcontrib>SARTORIUS, C</creatorcontrib><creatorcontrib>TAYLOR, B. A</creatorcontrib><creatorcontrib>PAYNE, A. H</creatorcontrib><title>Isolation, characterization, and chromosomal mapping of mouse P450 17α-hydroxylase/C17-20 lyase</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>Cytochrome P450 17 alpha-hydroxylase/C17-20 lyase (P45017 alpha) catalyzes the conversion of C-21 steroids to C-19 steroids in gonads. A full-length mouse cDNA encoding P450 17 alpha was isolated from a mouse Leydig cell library and characterized by restriction mapping and sequencing. The predicted amino acid sequence has 83% homology to rat, 66% homology to human, and 62% homology to bovine P45017 alpha amino acid sequences. The protein is 507 amino acids in length, which is 1 amino acid shorter than the human protein and 2 amino acids shorter than the bovine protein. The structural gene encoding P450 17 alpha (Cyp17) was localized utilizing an interspecific testcross to mouse chromosome 19, distal to Got-1. Another cytochrome P450, P4502c (Cyp2c), also is located at the distal end of chromosome 19. CYP17, CYP2c, and GOT1 have been mapped to human chromosome 10, with CYP2C and GOT1 mapped to the distal region, q24.3 and q25.3, respectively. The data in the present study indicate conserved syntenic loci on mouse chromosome 19 and human chromosome 10 and predict that the structural gene encoding P45017 alpha will be found distal to GOT1 on human chromosome 10.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>Crosses, Genetic</subject><subject>DNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes. Genome</subject><subject>Genetic Linkage</subject><subject>Humans</subject><subject>Leydig Cells</subject><subject>Male</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Restriction Mapping</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Steroid 17-alpha-Hydroxylase - genetics</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkNtKxDAQhoMouh7eQKE3ioLVmebQ5FKKhwVBL7yP2TTRStusyS64vpUv4jPZdRe9Gmb-A8NHyCHCBQKKS5BS5iVn9FThmQKOmFcbZIQgVS4FE5tk9GfZIbspvQGAorLYJtsoGXCuRuR5nEJrZk3ozzP7aqKxMxebz_XF9PVwjaELKXSmzToznTb9SxZ81oV5ctkj45Bh-f2Vvy7qGD4WrUnussIyLyBrF8OyT7a8aZM7WM898nRz_VTd5fcPt-Pq6j63tJSzvBQ1lcAcpxJrKCbIBHBfe4sUvaMArBBWFaCYcIqXE8-Nt-AlUocInu6Rk1XtNIb3uUsz3TXJurY1vRs-1RKGvgJxMLKV0caQUnReT2PTmbjQCHrJVS-h6SU0rVD_ctXVEDta988nnav_QyuQg3681k2ypvXR9LZJ_zYlCkpB0B8VsH8s</recordid><startdate>19910501</startdate><enddate>19910501</enddate><creator>YOUNGBLOOD, G. 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H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-76d3804e5381d02b14605fdfc131fe300426c920946e957bf5afc0f813e110f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>Crosses, Genetic</topic><topic>DNA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes. Genome</topic><topic>Genetic Linkage</topic><topic>Humans</topic><topic>Leydig Cells</topic><topic>Male</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Restriction Mapping</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Steroid 17-alpha-Hydroxylase - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>YOUNGBLOOD, G. L</creatorcontrib><creatorcontrib>SARTORIUS, C</creatorcontrib><creatorcontrib>TAYLOR, B. A</creatorcontrib><creatorcontrib>PAYNE, A. H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Genomics (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>YOUNGBLOOD, G. L</au><au>SARTORIUS, C</au><au>TAYLOR, B. A</au><au>PAYNE, A. H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation, characterization, and chromosomal mapping of mouse P450 17α-hydroxylase/C17-20 lyase</atitle><jtitle>Genomics (San Diego, Calif.)</jtitle><addtitle>Genomics</addtitle><date>1991-05-01</date><risdate>1991</risdate><volume>10</volume><issue>1</issue><spage>270</spage><epage>275</epage><pages>270-275</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>Cytochrome P450 17 alpha-hydroxylase/C17-20 lyase (P45017 alpha) catalyzes the conversion of C-21 steroids to C-19 steroids in gonads. A full-length mouse cDNA encoding P450 17 alpha was isolated from a mouse Leydig cell library and characterized by restriction mapping and sequencing. The predicted amino acid sequence has 83% homology to rat, 66% homology to human, and 62% homology to bovine P45017 alpha amino acid sequences. The protein is 507 amino acids in length, which is 1 amino acid shorter than the human protein and 2 amino acids shorter than the bovine protein. The structural gene encoding P450 17 alpha (Cyp17) was localized utilizing an interspecific testcross to mouse chromosome 19, distal to Got-1. Another cytochrome P450, P4502c (Cyp2c), also is located at the distal end of chromosome 19. CYP17, CYP2c, and GOT1 have been mapped to human chromosome 10, with CYP2C and GOT1 mapped to the distal region, q24.3 and q25.3, respectively. The data in the present study indicate conserved syntenic loci on mouse chromosome 19 and human chromosome 10 and predict that the structural gene encoding P45017 alpha will be found distal to GOT1 on human chromosome 10.</abstract><cop>San Diego, CA</cop><pub>Elsevier</pub><pmid>1840559</pmid><doi>10.1016/0888-7543(91)90511-C</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Amino Acid Sequence Animals Base Sequence Biological and medical sciences Blotting, Southern Crosses, Genetic DNA Fundamental and applied biological sciences. Psychology Genes. Genome Genetic Linkage Humans Leydig Cells Male Mice Molecular and cellular biology Molecular genetics Molecular Sequence Data Restriction Mapping Sequence Homology, Nucleic Acid Steroid 17-alpha-Hydroxylase - genetics |
| title | Isolation, characterization, and chromosomal mapping of mouse P450 17α-hydroxylase/C17-20 lyase |
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