Polyamines eliminate an extreme size bias against transformation of large yeast artificial chromosome DNA

The recent development of vectors and methods for cloning large linear DNA as yeast artificial chromosomes (YACs) has enormous potential in facilitating genome analysis, particularly because of the large cloning capacity of the YAC cloning system. However, the construction of comprehensive libraries...

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Veröffentlicht in:	Genomics (San Diego, Calif.) Calif.), 1991-05, Vol.10 (1), p.10-16
Hauptverfasser:	Connelly, Carla, McCormick, Mary Kay, Shero, James, Hieter, Philip
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Chromosomes, Fungal DNA - drug effects Fundamental and applied biological sciences. Psychology Gene Library Genetic engineering Genetic technics Genome, Human Humans Methods. Procedures. Technologies Molecular cloning Polyamines - pharmacology Transformation, Genetic
Online-Zugang:	Volltext
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