Yeast nucleosomal particles: structural and transcriptional properties
Yeast nucleosomal core particles have been characterized by thermal denaturation, circular dichroism, and digestion with DNase I and with trypsin. Practically all nucleosomal DNA melts in one transition centered at 70 degree C, and the circular dichroism spectrum is displaced to lower wavelengths as...
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| Veröffentlicht in: | Biochemistry (Easton) 1991-06, Vol.30 (23), p.5805-5810 |
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| creator | Pineiro, Manuel Puerta, Carmen Palacian, Enrique |
| description | Yeast nucleosomal core particles have been characterized by thermal denaturation, circular dichroism, and digestion with DNase I and with trypsin. Practically all nucleosomal DNA melts in one transition centered at 70 degree C, and the circular dichroism spectrum is displaced to lower wavelengths as compared to that corresponding to chicken nucleosomal cores. The susceptibility of yeast nucleosomal particles to dissociation by salt is significantly higher than that of chicken nucleosomal cores, a substantial dissociation being observed at 0.5 M NaCl. Treatment of yeast nucleosomal particles with the amino group reagent dimethylmaleic anhydride is accompanied by selective release of histones H2A and H2B. The results indicate not large but significant structural differences between yeast and chicken nucleosomal cores. However, the in vitro transcription properties of complete and H2A.H2B-deficient nucleosomal cores are similar in the two kinds of particles; the histone octamer blocks RNA synthesis, this block being eliminated in part by the partial loss of histones H2A and H2B |
| doi_str_mv | 10.1021/bi00237a025 |
| format | Article |
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Practically all nucleosomal DNA melts in one transition centered at 70 degree C, and the circular dichroism spectrum is displaced to lower wavelengths as compared to that corresponding to chicken nucleosomal cores. The susceptibility of yeast nucleosomal particles to dissociation by salt is significantly higher than that of chicken nucleosomal cores, a substantial dissociation being observed at 0.5 M NaCl. Treatment of yeast nucleosomal particles with the amino group reagent dimethylmaleic anhydride is accompanied by selective release of histones H2A and H2B. The results indicate not large but significant structural differences between yeast and chicken nucleosomal cores. 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Psychology ; HIDROLASAS ; Histones - chemistry ; Histones - genetics ; Hot Temperature ; HYDROLASE ; Maleic Anhydrides - pharmacology ; Microbiology ; Morphology, structure, chemical composition ; Mycology ; Nucleosomes - chemistry ; Protein Denaturation ; Saccharomyces ; SACCHAROMYCES CEREVISIAE ; Saccharomyces cerevisiae - drug effects ; Saccharomyces cerevisiae - genetics ; Sodium Chloride - pharmacology ; Transcription, Genetic</subject><ispartof>Biochemistry (Easton), 1991-06, Vol.30 (23), p.5805-5810</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a433t-11dbe1b30b5852ba71cd8ed200e6736293fa6c71eb508cb1a8a2a56aee199c93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bi00237a025$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bi00237a025$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5601744$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2043619$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pineiro, Manuel</creatorcontrib><creatorcontrib>Puerta, Carmen</creatorcontrib><creatorcontrib>Palacian, Enrique</creatorcontrib><title>Yeast nucleosomal particles: structural and transcriptional properties</title><title>Biochemistry (Easton)</title><addtitle>Biochemistry</addtitle><description>Yeast nucleosomal core particles have been characterized by thermal denaturation, circular dichroism, and digestion with DNase I and with trypsin. Practically all nucleosomal DNA melts in one transition centered at 70 degree C, and the circular dichroism spectrum is displaced to lower wavelengths as compared to that corresponding to chicken nucleosomal cores. The susceptibility of yeast nucleosomal particles to dissociation by salt is significantly higher than that of chicken nucleosomal cores, a substantial dissociation being observed at 0.5 M NaCl. Treatment of yeast nucleosomal particles with the amino group reagent dimethylmaleic anhydride is accompanied by selective release of histones H2A and H2B. The results indicate not large but significant structural differences between yeast and chicken nucleosomal cores. However, the in vitro transcription properties of complete and H2A.H2B-deficient nucleosomal cores are similar in the two kinds of particles; the histone octamer blocks RNA synthesis, this block being eliminated in part by the partial loss of histones H2A and H2B</description><subject>ADN</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chickens</subject><subject>CHROMATINE</subject><subject>Circular Dichroism</subject><subject>CROMATINA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HIDROLASAS</subject><subject>Histones - chemistry</subject><subject>Histones - genetics</subject><subject>Hot Temperature</subject><subject>HYDROLASE</subject><subject>Maleic Anhydrides - pharmacology</subject><subject>Microbiology</subject><subject>Morphology, structure, chemical composition</subject><subject>Mycology</subject><subject>Nucleosomes - chemistry</subject><subject>Protein Denaturation</subject><subject>Saccharomyces</subject><subject>SACCHAROMYCES CEREVISIAE</subject><subject>Saccharomyces cerevisiae - drug effects</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Sodium Chloride - pharmacology</subject><subject>Transcription, Genetic</subject><issn>0006-2960</issn><issn>1520-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS1U1G4XTr0hVdoDKgcUGNuxE3ODhfK1AqRdKnGyJo6D0maTxeNI8N_jVVYrDkg9WeP308ybN4xdcHjBQfCXVQsgZIEg1AM240pAlhujTtgMAHQmjIYzdk50m8ocivyUnQrIpeZmxq5_eKS46EfX-YGGLXaLHYbYppJeLSiG0cUxpF_s60UM2JML7S62Q78nw7DzCfb0iD1ssCP_-PDO2eb63Wb5IVt9ff9x-XqVYS5lzDivK88rCZUqlaiw4K4ufS0AvC6kFkY2qF3BfaWgdBXHEgUqjd5zY5yRc3Y1tU2Tf42eot225HzXYe-HkWwJGoRJu90Hcg1GQJo5Z88n0IWBKPjG7kK7xfDHcrD7dO0_6Sb68tB2rLa-PrKHOJP-9KAjOeyalJdr6YgpDbzI9-6yCWsp-t9HGcOdTZYKZTff1vbtzSf55c3nlV0n_snENzhY_BlSy-9rw5UW-X6DZ5OIjuztMIZ0Gfqv-782C6W8</recordid><startdate>19910611</startdate><enddate>19910611</enddate><creator>Pineiro, Manuel</creator><creator>Puerta, Carmen</creator><creator>Palacian, Enrique</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19910611</creationdate><title>Yeast nucleosomal particles: structural and transcriptional properties</title><author>Pineiro, Manuel ; Puerta, Carmen ; Palacian, Enrique</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a433t-11dbe1b30b5852ba71cd8ed200e6736293fa6c71eb508cb1a8a2a56aee199c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>ADN</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chickens</topic><topic>CHROMATINE</topic><topic>Circular Dichroism</topic><topic>CROMATINA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HIDROLASAS</topic><topic>Histones - chemistry</topic><topic>Histones - genetics</topic><topic>Hot Temperature</topic><topic>HYDROLASE</topic><topic>Maleic Anhydrides - pharmacology</topic><topic>Microbiology</topic><topic>Morphology, structure, chemical composition</topic><topic>Mycology</topic><topic>Nucleosomes - chemistry</topic><topic>Protein Denaturation</topic><topic>Saccharomyces</topic><topic>SACCHAROMYCES CEREVISIAE</topic><topic>Saccharomyces cerevisiae - drug effects</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Sodium Chloride - pharmacology</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pineiro, Manuel</creatorcontrib><creatorcontrib>Puerta, Carmen</creatorcontrib><creatorcontrib>Palacian, Enrique</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemistry (Easton)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pineiro, Manuel</au><au>Puerta, Carmen</au><au>Palacian, Enrique</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Yeast nucleosomal particles: structural and transcriptional properties</atitle><jtitle>Biochemistry (Easton)</jtitle><addtitle>Biochemistry</addtitle><date>1991-06-11</date><risdate>1991</risdate><volume>30</volume><issue>23</issue><spage>5805</spage><epage>5810</epage><pages>5805-5810</pages><issn>0006-2960</issn><eissn>1520-4995</eissn><abstract>Yeast nucleosomal core particles have been characterized by thermal denaturation, circular dichroism, and digestion with DNase I and with trypsin. Practically all nucleosomal DNA melts in one transition centered at 70 degree C, and the circular dichroism spectrum is displaced to lower wavelengths as compared to that corresponding to chicken nucleosomal cores. The susceptibility of yeast nucleosomal particles to dissociation by salt is significantly higher than that of chicken nucleosomal cores, a substantial dissociation being observed at 0.5 M NaCl. Treatment of yeast nucleosomal particles with the amino group reagent dimethylmaleic anhydride is accompanied by selective release of histones H2A and H2B. The results indicate not large but significant structural differences between yeast and chicken nucleosomal cores. 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| ispartof | Biochemistry (Easton), 1991-06, Vol.30 (23), p.5805-5810 |
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| language | eng |
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| subjects | ADN Animals Biological and medical sciences Chickens CHROMATINE Circular Dichroism CROMATINA Fundamental and applied biological sciences. Psychology HIDROLASAS Histones - chemistry Histones - genetics Hot Temperature HYDROLASE Maleic Anhydrides - pharmacology Microbiology Morphology, structure, chemical composition Mycology Nucleosomes - chemistry Protein Denaturation Saccharomyces SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - genetics Sodium Chloride - pharmacology Transcription, Genetic |
| title | Yeast nucleosomal particles: structural and transcriptional properties |
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