Amino acid sequence similarities of the mitochondrial short chain delta 3, delta 2-enoyl-CoA isomerase and peroxisomal multifunctional delta 3, delta 2-enoyl-CoA isomerase, 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase enzyme in rat liver. The proposed occurrence of isomerization and hydration in the same catalytic domain of the multifunctional enzyme

We report the isolation and characterization of a cDNA encoding the mitochondrial short chain delta 3, delta 2-enoyl-CoA isomerase from rat liver. Tryptic fragments of the purified protein were generated, purified, and sequenced. A rat liver cDNA library, constructed in the plasmid vector pUEX1 was...

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Veröffentlicht in:The Journal of biological chemistry 1991-06, Vol.266 (17), p.10750-10753
Hauptverfasser: Palosaari, P M, Vihinen, M, Mäntsälä, P I, Alexson, S E, Pihlajaniemi, T, Hiltunen, J K
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Sprache:eng
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