Probing the functional role and localization of Escherichia coli ribosomal protein L16 with a monoclonal antibody

A monoclonal antibody specific for Escherichia coli ribosomal protein L16 was prepared to test its effects on ribosome function and to locate L16 by immunoelectron microscopy. The antibody recognized L16 in 50 S subunits, but not in 70 S ribosomes. It inhibited association of ribosomal subunits at 1...

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Veröffentlicht in:	The Journal of biological chemistry 1991-06, Vol.266 (17), p.11116-11121
Hauptverfasser:	NAG, B, GLITZ, D. G, TEWARI, D. S, TRAUT, R. R
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Monoclonal Biological and medical sciences Cell structures and functions Escherichia coli - metabolism Escherichia coli - ultrastructure Fundamental and applied biological sciences. Psychology Immunoglobulin Fab Fragments Microscopy, Electron Models, Structural Molecular and cellular biology Peptide Biosynthesis Peptides Poly U - metabolism Ribosomal Proteins - analysis Ribosomal Proteins - metabolism Ribosomal Proteins - ultrastructure Ribosome Ribosomes - metabolism Ribosomes - ultrastructure
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