A novel secreted cyclophilin-like protein (SCYLP)
A novel cyclosporin A binding glycoprotein of 21 kDa was isolated from human milk by several steps of cation exchange chromatography. The corresponding gene was cloned from human T cells, expressed in Escherichia coli and the recombinant protein purified. The protein shares 58% amino acid identity w...
Gespeichert in:
| Veröffentlicht in: | The Journal of biological chemistry 1991-06, Vol.266 (17), p.10735-10738 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 10738 |
|---|---|
| container_issue | 17 |
| container_start_page | 10735 |
| container_title | The Journal of biological chemistry |
| container_volume | 266 |
| creator | SPIK, G HAENDLER, B KELLER, R HIESTAND, P. C MOVVA, N. R DELMAS, O MARILLER, C CHAMOUX, M MAES, P TARTAR, A MONTREUIL, J STEDMAN, K KOCHER, H. P |
| description | A novel cyclosporin A binding glycoprotein of 21 kDa was isolated from human milk by several steps of cation exchange chromatography.
The corresponding gene was cloned from human T cells, expressed in Escherichia coli and the recombinant protein purified.
The protein shares 58% amino acid identity with the cytosolic cyclophilin and is initially synthesized with a hydrophobic
leader sequence. The cyclophilin-like protein has also peptidyl-prolyl cis/trans-isomerase activity, although less efficient,
that is inhibited by cyclosporin A. The existence of a secreted form of cyclophilin-like protein in addition to the previously
known cytosolic cyclophilin implies that these proteins act on different in vivo targets. |
| doi_str_mv | 10.1016/S0021-9258(18)99078-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_80587613</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>80587613</sourcerecordid><originalsourceid>FETCH-LOGICAL-c409t-40e7049e6db232984a8c7343fadab957d0f80050d6504835028bbf97bc2f3c1e3</originalsourceid><addsrcrecordid>eNpFkE1Lw0AQhhdRaq3-hEIOIu0hOrubTXaPpfgFBYUq6GnZbCZmNU1qNlX6700_qHOZwzwz7_AQMqRwTYHGN3MARkPFhBxROVYKEhmyI9KnIHnIBX07Jv0DckrOvP-EriJFe6THIAKhWJ_QSVDVP1gGHm2DLWaBXduyXhaudFVYui8Mlk3doquC0Xz6Pnsen5OT3JQeL_Z9QF7vbl-mD-Hs6f5xOpmFNgLVhhFg0oVhnKWMMyUjI23CI56bzKRKJBnkEkBAFguIJBfAZJrmKkkty7mlyAfkane3y_9eoW_1wnmLZWkqrFdeSxAyiSnvQLEDbVN732Cul41bmGatKeiNKr1VpTceNJV6q0qzbm-4D1ilC8wOW3s33fxyPzfemjJvTGWdP2CRijvV8T9WuI_i1zWoU1fbAheaxbGmSfdCwgX_A9ZNelw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>80587613</pqid></control><display><type>article</type><title>A novel secreted cyclophilin-like protein (SCYLP)</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>SPIK, G ; HAENDLER, B ; KELLER, R ; HIESTAND, P. C ; MOVVA, N. R ; DELMAS, O ; MARILLER, C ; CHAMOUX, M ; MAES, P ; TARTAR, A ; MONTREUIL, J ; STEDMAN, K ; KOCHER, H. P</creator><creatorcontrib>SPIK, G ; HAENDLER, B ; KELLER, R ; HIESTAND, P. C ; MOVVA, N. R ; DELMAS, O ; MARILLER, C ; CHAMOUX, M ; MAES, P ; TARTAR, A ; MONTREUIL, J ; STEDMAN, K ; KOCHER, H. P</creatorcontrib><description>A novel cyclosporin A binding glycoprotein of 21 kDa was isolated from human milk by several steps of cation exchange chromatography.
The corresponding gene was cloned from human T cells, expressed in Escherichia coli and the recombinant protein purified.
The protein shares 58% amino acid identity with the cytosolic cyclophilin and is initially synthesized with a hydrophobic
leader sequence. The cyclophilin-like protein has also peptidyl-prolyl cis/trans-isomerase activity, although less efficient,
that is inhibited by cyclosporin A. The existence of a secreted form of cyclophilin-like protein in addition to the previously
known cytosolic cyclophilin implies that these proteins act on different in vivo targets.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(18)99078-2</identifier><identifier>PMID: 2040592</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>Amino Acid Isomerases - genetics ; Amino Acid Isomerases - isolation & purification ; Amino Acid Isomerases - metabolism ; Amino Acid Sequence ; Analytical, structural and metabolic biochemistry ; Base Sequence ; Binding and carrier proteins ; Biological and medical sciences ; Carrier Proteins - genetics ; Carrier Proteins - isolation & purification ; Carrier Proteins - metabolism ; Chromatography, Gel ; Cyclosporins - metabolism ; Escherichia coli - genetics ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; Kinetics ; Milk, Human - chemistry ; Molecular Sequence Data ; Molecular Weight ; Oligonucleotide Probes ; Peptidylprolyl Isomerase ; Proteins ; Recombinant Proteins - isolation & purification ; Recombinant Proteins - metabolism ; Sequence Homology, Nucleic Acid</subject><ispartof>The Journal of biological chemistry, 1991-06, Vol.266 (17), p.10735-10738</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-40e7049e6db232984a8c7343fadab957d0f80050d6504835028bbf97bc2f3c1e3</citedby><cites>FETCH-LOGICAL-c409t-40e7049e6db232984a8c7343fadab957d0f80050d6504835028bbf97bc2f3c1e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4961086$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2040592$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SPIK, G</creatorcontrib><creatorcontrib>HAENDLER, B</creatorcontrib><creatorcontrib>KELLER, R</creatorcontrib><creatorcontrib>HIESTAND, P. C</creatorcontrib><creatorcontrib>MOVVA, N. R</creatorcontrib><creatorcontrib>DELMAS, O</creatorcontrib><creatorcontrib>MARILLER, C</creatorcontrib><creatorcontrib>CHAMOUX, M</creatorcontrib><creatorcontrib>MAES, P</creatorcontrib><creatorcontrib>TARTAR, A</creatorcontrib><creatorcontrib>MONTREUIL, J</creatorcontrib><creatorcontrib>STEDMAN, K</creatorcontrib><creatorcontrib>KOCHER, H. P</creatorcontrib><title>A novel secreted cyclophilin-like protein (SCYLP)</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>A novel cyclosporin A binding glycoprotein of 21 kDa was isolated from human milk by several steps of cation exchange chromatography.
The corresponding gene was cloned from human T cells, expressed in Escherichia coli and the recombinant protein purified.
The protein shares 58% amino acid identity with the cytosolic cyclophilin and is initially synthesized with a hydrophobic
leader sequence. The cyclophilin-like protein has also peptidyl-prolyl cis/trans-isomerase activity, although less efficient,
that is inhibited by cyclosporin A. The existence of a secreted form of cyclophilin-like protein in addition to the previously
known cytosolic cyclophilin implies that these proteins act on different in vivo targets.</description><subject>Amino Acid Isomerases - genetics</subject><subject>Amino Acid Isomerases - isolation & purification</subject><subject>Amino Acid Isomerases - metabolism</subject><subject>Amino Acid Sequence</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Base Sequence</subject><subject>Binding and carrier proteins</subject><subject>Biological and medical sciences</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - isolation & purification</subject><subject>Carrier Proteins - metabolism</subject><subject>Chromatography, Gel</subject><subject>Cyclosporins - metabolism</subject><subject>Escherichia coli - genetics</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Milk, Human - chemistry</subject><subject>Molecular Sequence Data</subject><subject>Molecular Weight</subject><subject>Oligonucleotide Probes</subject><subject>Peptidylprolyl Isomerase</subject><subject>Proteins</subject><subject>Recombinant Proteins - isolation & purification</subject><subject>Recombinant Proteins - metabolism</subject><subject>Sequence Homology, Nucleic Acid</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1Lw0AQhhdRaq3-hEIOIu0hOrubTXaPpfgFBYUq6GnZbCZmNU1qNlX6700_qHOZwzwz7_AQMqRwTYHGN3MARkPFhBxROVYKEhmyI9KnIHnIBX07Jv0DckrOvP-EriJFe6THIAKhWJ_QSVDVP1gGHm2DLWaBXduyXhaudFVYui8Mlk3doquC0Xz6Pnsen5OT3JQeL_Z9QF7vbl-mD-Hs6f5xOpmFNgLVhhFg0oVhnKWMMyUjI23CI56bzKRKJBnkEkBAFguIJBfAZJrmKkkty7mlyAfkane3y_9eoW_1wnmLZWkqrFdeSxAyiSnvQLEDbVN732Cul41bmGatKeiNKr1VpTceNJV6q0qzbm-4D1ilC8wOW3s33fxyPzfemjJvTGWdP2CRijvV8T9WuI_i1zWoU1fbAheaxbGmSfdCwgX_A9ZNelw</recordid><startdate>19910615</startdate><enddate>19910615</enddate><creator>SPIK, G</creator><creator>HAENDLER, B</creator><creator>KELLER, R</creator><creator>HIESTAND, P. C</creator><creator>MOVVA, N. R</creator><creator>DELMAS, O</creator><creator>MARILLER, C</creator><creator>CHAMOUX, M</creator><creator>MAES, P</creator><creator>TARTAR, A</creator><creator>MONTREUIL, J</creator><creator>STEDMAN, K</creator><creator>KOCHER, H. P</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19910615</creationdate><title>A novel secreted cyclophilin-like protein (SCYLP)</title><author>SPIK, G ; HAENDLER, B ; KELLER, R ; HIESTAND, P. C ; MOVVA, N. R ; DELMAS, O ; MARILLER, C ; CHAMOUX, M ; MAES, P ; TARTAR, A ; MONTREUIL, J ; STEDMAN, K ; KOCHER, H. P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-40e7049e6db232984a8c7343fadab957d0f80050d6504835028bbf97bc2f3c1e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Amino Acid Isomerases - genetics</topic><topic>Amino Acid Isomerases - isolation & purification</topic><topic>Amino Acid Isomerases - metabolism</topic><topic>Amino Acid Sequence</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Base Sequence</topic><topic>Binding and carrier proteins</topic><topic>Biological and medical sciences</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - isolation & purification</topic><topic>Carrier Proteins - metabolism</topic><topic>Chromatography, Gel</topic><topic>Cyclosporins - metabolism</topic><topic>Escherichia coli - genetics</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Milk, Human - chemistry</topic><topic>Molecular Sequence Data</topic><topic>Molecular Weight</topic><topic>Oligonucleotide Probes</topic><topic>Peptidylprolyl Isomerase</topic><topic>Proteins</topic><topic>Recombinant Proteins - isolation & purification</topic><topic>Recombinant Proteins - metabolism</topic><topic>Sequence Homology, Nucleic Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SPIK, G</creatorcontrib><creatorcontrib>HAENDLER, B</creatorcontrib><creatorcontrib>KELLER, R</creatorcontrib><creatorcontrib>HIESTAND, P. C</creatorcontrib><creatorcontrib>MOVVA, N. R</creatorcontrib><creatorcontrib>DELMAS, O</creatorcontrib><creatorcontrib>MARILLER, C</creatorcontrib><creatorcontrib>CHAMOUX, M</creatorcontrib><creatorcontrib>MAES, P</creatorcontrib><creatorcontrib>TARTAR, A</creatorcontrib><creatorcontrib>MONTREUIL, J</creatorcontrib><creatorcontrib>STEDMAN, K</creatorcontrib><creatorcontrib>KOCHER, H. P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SPIK, G</au><au>HAENDLER, B</au><au>KELLER, R</au><au>HIESTAND, P. C</au><au>MOVVA, N. R</au><au>DELMAS, O</au><au>MARILLER, C</au><au>CHAMOUX, M</au><au>MAES, P</au><au>TARTAR, A</au><au>MONTREUIL, J</au><au>STEDMAN, K</au><au>KOCHER, H. P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel secreted cyclophilin-like protein (SCYLP)</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1991-06-15</date><risdate>1991</risdate><volume>266</volume><issue>17</issue><spage>10735</spage><epage>10738</epage><pages>10735-10738</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>A novel cyclosporin A binding glycoprotein of 21 kDa was isolated from human milk by several steps of cation exchange chromatography.
The corresponding gene was cloned from human T cells, expressed in Escherichia coli and the recombinant protein purified.
The protein shares 58% amino acid identity with the cytosolic cyclophilin and is initially synthesized with a hydrophobic
leader sequence. The cyclophilin-like protein has also peptidyl-prolyl cis/trans-isomerase activity, although less efficient,
that is inhibited by cyclosporin A. The existence of a secreted form of cyclophilin-like protein in addition to the previously
known cytosolic cyclophilin implies that these proteins act on different in vivo targets.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>2040592</pmid><doi>10.1016/S0021-9258(18)99078-2</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1991-06, Vol.266 (17), p.10735-10738 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80587613 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino Acid Isomerases - genetics Amino Acid Isomerases - isolation & purification Amino Acid Isomerases - metabolism Amino Acid Sequence Analytical, structural and metabolic biochemistry Base Sequence Binding and carrier proteins Biological and medical sciences Carrier Proteins - genetics Carrier Proteins - isolation & purification Carrier Proteins - metabolism Chromatography, Gel Cyclosporins - metabolism Escherichia coli - genetics Female Fundamental and applied biological sciences. Psychology Humans Kinetics Milk, Human - chemistry Molecular Sequence Data Molecular Weight Oligonucleotide Probes Peptidylprolyl Isomerase Proteins Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Sequence Homology, Nucleic Acid |
| title | A novel secreted cyclophilin-like protein (SCYLP) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T03%3A24%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20novel%20secreted%20cyclophilin-like%20protein%20(SCYLP)&rft.jtitle=The%20Journal%20of%20biological%20chemistry&rft.au=SPIK,%20G&rft.date=1991-06-15&rft.volume=266&rft.issue=17&rft.spage=10735&rft.epage=10738&rft.pages=10735-10738&rft.issn=0021-9258&rft.eissn=1083-351X&rft.coden=JBCHA3&rft_id=info:doi/10.1016/S0021-9258(18)99078-2&rft_dat=%3Cproquest_cross%3E80587613%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=80587613&rft_id=info:pmid/2040592&rfr_iscdi=true |